BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: TAIR10_cdna_20110103_representative_gene_model_updated 33,602 sequences; 51,074,197 total letters Query= Ahg947835 Length=2073 Score E Sequences producing significant alignments: (Bits) Value AT4G30870.1 | Symbols: ATMUS81, MUS81 | Restriction endonucleas... 1103 0.0 AT5G39770.1 | Symbols: | Restriction endonuclease, type II-lik... 638 0.0 AT5G41940.1 | Symbols: | Ypt/Rab-GAP domain of gyp1p superfami... 178 2e-43 > AT4G30870.1 | Symbols: ATMUS81, MUS81 | Restriction endonuclease, type II-like superfamily protein | chr4:15028427-15032634 FORWARD LENGTH=2312 Length=2312 Score = 1103 bits (597), Expect = 0.0 Identities = 851/969 (88%), Gaps = 35/969 (4%) Strand=Plus/Plus Query 842 GTCAAGTTGATCTTGAGGGATCTTTC-T-CTAAGAAATTCCGTTCTTGCAATGTTGGATC 899 |||||||||||||||||||||| || | ||||||| ||||| |||||||||| |||||| Sbjct 1163 GTCAAGTTGATCTTGAGGGATC--TCGTGCTAAGAAGTTCCGCTCTTGCAATGATGGATC 1220 Query 900 CACCTTAAATCCATGTTCTTCCGGTTCGAGCCATGCCATGAAAGCCTGCTCATCTTCCTT 959 |||||||||||||||||| || ||||| || ||||| ||||||||||||||||||| || Sbjct 1221 CACCTTAAATCCATGTTCATCAGGTTCAAGTCATGCTGTGAAAGCCTGCTCATCTTCTTT 1280 Query 960 GGCGTCCGATGGAACCGAAGGAGTGACAACTATTACACGCCTTCCACCTCTT-AGATTCG 1018 ||| || ||||||||| ||||| | |||| |||| ||||||| || |||||| | ||| | Sbjct 1281 GGCATCAGATGGAACCAAAGGAATAACAAATATTCCACGCCTCCCTCCTCTTCA-ATTTG 1339 Query 1019 GAGAAGCGTTTGAGGAAGCATATGATGTGATCTTGTTACTGGATGATCGAGAACAGTTTG 1078 ||||| ||||||||||||||||||||||||||||| ||||||||||||||||| |||||| Sbjct 1340 GAGAAACGTTTGAGGAAGCATATGATGTGATCTTGATACTGGATGATCGAGAAAAGTTTG 1399 Query 1079 CCACCAAGGGATCACGGTCCA----TA--GTTGATAACATACGCTCTGTATTCAAGATCA 1132 ||||||| |||||||| |||| || ||||| |||||| |||||| |||||| |||| Sbjct 1400 CCACCAAAGGATCACGATCCAGGAATATAGTTGAAAACATATGCTCTGAATTCAACATCA 1459 Query 1133 AGATTGAGGTTAGAAGATTACCAGTTGGGGATTGTATCTGGATTGCTCGGCATAAGTACC 1192 |||||||||||||| |||||||||| ||||||||||| |||||||||||||||||||||| Sbjct 1460 AGATTGAGGTTAGACGATTACCAGTCGGGGATTGTATATGGATTGCTCGGCATAAGTACC 1519 Query 1193 AACAAGA-TGAATATGTTCTGGACTTTATTGTTGAGCGGAAGAACGTTGATGATATGCGC 1251 | || |||||||||||| |||||||||| |||| ||||||||||||||||||||||| Sbjct 1520 TTGA-GACTGAATATGTTCTAGACTTTATTGCTGAGAGGAAGAACGTTGATGATATGCGC 1578 Query 1252 TCGTCAATTATG-GATAATCGCTACA-AGGACCAAAAACTTAGACTTCAGAGATCAGGAA 1309 || ||||||| | ||||||||||||| || ||||||||||||||||||||||||| ||| Sbjct 1579 TCATCAATTA-GAGATAATCGCTACAGAG-ACCAAAAACTTAGACTTCAGAGATCGGGAT 1636 Query 1310 TCAAGAAGCTGATATACATTCTTGAAGGGGACCCAAACCAGTCT-AGTACAGCAGAAAGT 1368 |||||||||| |||||||||||||||||||| |||||||| ||| | | |||||||||| Sbjct 1637 TCAAGAAGCTCATATACATTCTTGAAGGGGATCCAAACCATTCTGA-TGCAGCAGAAAGC 1695 Query 1369 ATTAAAACGGCTTGTTTCACAACGGAGATTTTGGAAGGATTTGATGTGCTGAGGACAAAC 1428 |||||||| ||||||||||| || |||||| |||| ||||||||||||||||||||| || Sbjct 1696 ATTAAAACAGCTTGTTTCACGACAGAGATTCTGGAGGGATTTGATGTGCTGAGGACACAC 1755 Query 1429 GGGCTGGGTGAGACGTTAAGAAAATACGGTTATCTCACTAAATCAATATACCAATACTAC 1488 ||||||||||||||| ||||||||||||| || ||||| ||||||||||| ||||||||| Sbjct 1756 GGGCTGGGTGAGACGCTAAGAAAATACGGCTACCTCACGAAATCAATATATCAATACTAC 1815 Query 1489 AAGTCACGGGTGAATGAT---GACCAGAGCAAAGTTGC-G--T---GTCCTTCTTTCGAT 1539 |||| ||||||||||||| ||||||||||||| || | | |||||||||| ||| Sbjct 1816 AAGTTACGGGTGAATGATAATGACCAGAGCAAAGGCGCAGCCTCATGTCCTTCTTTTGAT 1875 Query 1540 -GATTTTGTCAAAAGGTGTCAAGACCTTGGTAAAATGACAATCAGCGACGTATTTGCCAT 1598 | |||||||||||||||||||||||||| |||||||||||||||||| ||||||||||| Sbjct 1876 AG-TTTTGTCAAAAGGTGTCAAGACCTTGATAAAATGACAATCAGCGATGTATTTGCCAT 1934 Query 1599 TCAGCTTATGCAGATACCGCAGGTAACAGAGGAAATTGCCATAGCTGTTCTTGATAAGTA 1658 |||||| |||||| | ||||||||||| ||||||||||||||||| |||||||||| ||| Sbjct 1935 TCAGCTAATGCAGGTCCCGCAGGTAACGGAGGAAATTGCCATAGCGGTTCTTGATATGTA 1994 Query 1659 CAAAACACTTCGGTCTCTTGCTTCTGCCTATTCTAAACTAGATGG-GAATGTCTCGGCGC 1717 | ||| ||| |||||||||||||||||||||| | ||||| | || ||||||||||| Sbjct 1995 CCCAACGCTTTTGTCTCTTGCTTCTGCCTATTCTCATCTAGAAGCAGA-TGTCTCGGCGC 2053 Query 1718 AAGAAGAAATGCTGCGGAATCA-AAGCAACAATGTAATATGTGCATCAGCTAGTAAGAAT 1776 ||||||| ||| || | ||| | ||||||||||||||||||||||||||||||||||||| Sbjct 2054 AAGAAGAGATGTTGAGAAAT-AGAAGCAACAATGTAATATGTGCATCAGCTAGTAAGAAT 2112 Query 1777 ATATACAAG 1785 |||| |||| Sbjct 2113 ATATTCAAG 2121 Score = 795 bits (430), Expect = 0.0 Identities = 690/814 (85%), Gaps = 23/814 (3%) Strand=Plus/Plus Query 1 AACTCTTTGGAGTGTGTAGTGATGGTTGACCAGAGAAGGGTACTGTGTTCACAGAACCAA 60 |||| ||||||||| |||||||||| |||| ||||| |||||||||| | |||||| | Sbjct 139 AACTATTTGGAGTGGGTAGTGATGGATGACGAGAGACGGGTACTGTGCCCTGAGAACCGA 198 Query 61 GGTTTAGCGGAC-TATGTGTTGCAGAAGAAGCAAGAACACGAAGATAAGCCTAAGGGTTT 119 |||||||||| | |||||||||||||||||||||||| ||| ||| ||||| || || || Sbjct 199 GGTTTAGCGG-CTTATGTGTTGCAGAAGAAGCAAGAATACGCAGAAAAGCCCAAAGGCTT 257 Query 120 GTCAGAAAATCTGGAGAGGACATTTGTTAAAGCCTATAGAAATGTATGTGACGCCAAGGA 179 ||| |||||||||||||||||||||||||| | ||||| | ||| || ||||||||||| Sbjct 258 GTCGGAAAATCTGGAGAGGACATTTGTTAAGGGGTATAGGAGTGTCTGCGACGCCAAGGA 317 Query 180 TTCAATTGACACCCTCAAAGACTTGTCGAAGATCAAGGGCTTTGGCAAGTGGATGGTTAA 239 | | ||| |||| ||||||||||||||| ||||||||||||||||||||||||||||||| Sbjct 318 TCCCATTAACACACTCAAAGACTTGTCGCAGATCAAGGGCTTTGGCAAGTGGATGGTTAA 377 Query 240 GCTCATGCAAGAATATTTTG-T--TAC---TG---G-TC----C-TGAAGCTGTACCTGG 284 ||||||| ||| |||||||| | | | || | || | |||| | ||||| | Sbjct 378 GCTCATGAAAGGATATTTTGATACTGCTGTTGAAAGTTCTGAGCAAGAAGATTTACCT-G 436 Query 285 A-AATCGTGCTGGGAAAAAGGCCAAGGGAACAGAACCCTACAGGCCGCGAAGAAATTCTG 343 | ||||||||||||||||||||||| |||| | ||| |||| |||| ||||||||| | Sbjct 437 ATAATCGTGCTGGGAAAAAGGCCAATGGAAAAAAACGGTACATTCCGCAAAGAAATTCAG 496 Query 344 CGGCTTATGCATTACTAATAACATTGCACAG-AGAGACTGCAAATGGGAAAGCATTCATG 402 || ||||||||||||||||| |||||||| || |||| |||||||||||| ||||||| Sbjct 497 TGGGATATGCATTACTAATAACGTTGCACAGAAG-GACTACAAATGGGAAAGAATTCATG 555 Query 403 CGTAAACAAGAGCTTATGGACGCTGCTGACGCTAGTGGGCTCTCACACGTTCCTATTGCA 462 ||||||||||||||||| || ||||||||||| | |||||| ||||||| |||| ||| Sbjct 556 CGTAAACAAGAGCTTATTGATGCTGCTGACGCGAATGGGCTATCACACGCTCCTGTTGGG 615 Query 463 CCAGAGAAAGGTAAAGGAAAAGTAGGAGTTGGAAACTCTAAGAGGGAGTGGTATAGCGGG 522 ||||||||||| ||||| |||| |||| ||||| |||||||||||||||||||||| || Sbjct 616 CCAGAGAAAGGGAAAGGGAAAGCAGGACTTGGACACTCTAAGAGGGAGTGGTATAGTGGA 675 Query 523 TGGAGCTGCATGAAGACACTTATAGAGAAGGGATTGGTGGTGAAATCTAGCAACCCGGCA 582 ||||||||||||| || |||||| |||||||||| |||||||||||||||||||| ||| Sbjct 676 TGGAGCTGCATGACCACTCTTATACAGAAGGGATTAGTGGTGAAATCTAGCAACCCAGCA 735 Query 583 AAGTACATGCTGACAGTTGAAGGTCGG-AATGTAGCTGATGACTGTATCGTGCGATCTGG 641 ||||||||||||||||||||||||||| || || || |||| |||||| |||||||||| Sbjct 736 AAGTACATGCTGACAGTTGAAGGTCGGGAA-GTTGCAAATGAGTGTATCCTGCGATCTGG 794 Query 642 ATTACTTAATTCGGTTGACATCTTATCTGATGATGAAATGGATCCTGTACAGCAAGCAAA 701 ||||| | |||| ||||| |||||||||| |||||||||||||||| || ||||||||| Sbjct 795 ATTACCTGATTCAGTTGATATCTTATCTGTTGATGAAATGGATCCTACACCGCAAGCAAA 854 Query 702 TATACCACCTAATCAAAATTCAACCTCCTCTTTTACCATGAGAGAAGAACAGCCGTATGT 761 | | |||||||||||||| | |||| || ||||||||||||||||||| || ||||| Sbjct 855 GAAAACACCTAATCAAAATCCCACCTGTTCGTTTACCATGAGAGAAGAACTTCCATATGT 914 Query 762 CGATGCAAGATCCAGGGGACAATCATCCATTCCA 795 |||| |||||| |||| ||||||| |||||||| Sbjct 915 CGATCCAAGATGTAGGGCACAATCAGCCATTCCA 948 > AT5G39770.1 | Symbols: | Restriction endonuclease, type II-like superfamily protein | chr5:15919241-15927549 REVERSE LENGTH=3819 Length=3819 Score = 638 bits (345), Expect = 0.0 Identities = 411/442 (93%), Gaps = 8/442 (2%) Strand=Plus/Plus Query 1181 GGCATAAGTACCAACAAGATGAATATGTTCTGGACTTTATTGTTGAGCGGAAGAACGTTG 1240 ||||||||||||||||||||||||||||||| ||||||||||||||| |||||||||||| Sbjct 3125 GGCATAAGTACCAACAAGATGAATATGTTCTCGACTTTATTGTTGAGAGGAAGAACGTTG 3184 Query 1241 ATGATATGCGCTCGTCAATTATGGATAATCGCTACAAGGACCAAAAACTTAGACTTCAGA 1300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3185 ATGATATGCGCTCGTCAATTATGGATAATCGCTACAAGGACCAAAAACTTAGACTTCAGA 3244 Query 1301 GATCAGGAATCAAGAAGCTGATATACATTCTTGAAGGGGACCCAAACCAGTCTA-GTACA 1359 |||| |||||||| |||||||||||||||||||||||||| ||||||||||||| | || Sbjct 3245 GATCGGGAATCAAAAAGCTGATATACATTCTTGAAGGGGATCCAAACCAGTCTAAG-ACT 3303 Query 1360 GCAGAAAGTATTAAAACGGCTTGTTTCACAACGGAGATTTTGGAAGGATTTGATGTGCTG 1419 |||||||| |||||||||||||||||||| |||||||| |||||||| ||||||||| || Sbjct 3304 GCAGAAAGCATTAAAACGGCTTGTTTCACTACGGAGATATTGGAAGGGTTTGATGTGGTG 3363 Query 1420 AGGACAAACGGGCTGGGTGAGACGTTAAGAAAATACGGTTATCTCACTAAATCAATATAC 1479 || |||||||| |||||||||||| |||||||||||||||||||||||||||| |||||| Sbjct 3364 AGAACAAACGGACTGGGTGAGACGCTAAGAAAATACGGTTATCTCACTAAATCGATATAC 3423 Query 1480 CAATACTACAAGTCACGGGTGAATGATGACCAGAGCAAAGTTGCG---T-GT--CCTTCT 1533 |||||||||||||| | |||||||||||||||||| ||||||||| | || |||||| Sbjct 3424 CAATACTACAAGTCGCTGGTGAATGATGACCAGAGGAAAGTTGCGGACTCGTGTCCTTCT 3483 Query 1534 TTCGATGATTTTGTCAAAAGGTGTCAAGACCTTGGTAAAATGACAATCAGCGACGTATTT 1593 | ||||||||||||| ||||||||| ||||||||||||||||||||||||||| |||||| Sbjct 3484 TACGATGATTTTGTCCAAAGGTGTCCAGACCTTGGTAAAATGACAATCAGCGATGTATTT 3543 Query 1594 GCCATTCAGCTTATGCAGATAC 1615 |||||||||||||||||| ||| Sbjct 3544 GCCATTCAGCTTATGCAGGTAC 3565 Score = 628 bits (340), Expect = 5e-179 Identities = 478/541 (88%), Gaps = 23/541 (4%) Strand=Plus/Plus Query 104 ATAAGCCTAAGGGTTTGTCAGAAAATCTGGAGAGGACATTTGTTAAAGCCTATAGAAATG 163 ||||||| ||||||||||| ||| ||| ||||| || ||||||||||||||| ||||||| Sbjct 2279 ATAAGCCCAAGGGTTTGTCCGAATATCAGGAGATGAAATTTGTTAAAGCCTACAGAAATG 2338 Query 164 TATGTGACGCCAAGGATTCAATTGACACCCTCAAAGACTTGTCGAAGATCAAGGGCTTTG 223 | || |||||||||||||||||||| ||||||||||||||||||||||||||||| |||| Sbjct 2339 TCTGCGACGCCAAGGATTCAATTGATACCCTCAAAGACTTGTCGAAGATCAAGGGATTTG 2398 Query 224 GCAAGTGGATGGTTAAGCTCATGCAAGAATATTTTGTTACTGGT-C--C----T------ 270 |||||| ||||||||||||||||||| |||||||||||||||| | | | Sbjct 2399 GCAAGTATATGGTTAAGCTCATGCAAGGATATTTTGTTACTGGTGCTGCAAGTTCCGAGC 2458 Query 271 --GAAGCTGTACCTGGAAATCGTGCTGGGAAAAAGGCCAAGGGAACAGAACCCTACAGGC 328 |||||| ||||||||||||||||||||||||||||||||||| ||||||| |||| || Sbjct 2459 AAGAAGCTTTACCTGGAAATCGTGCTGGGAAAAAGGCCAAGGGATCAGAACCGTACAAGC 2518 Query 329 CGCGAAGAAATTCTGCGGCTTATGCATTACTAATAACATTGCACA-G---AGAGACTGCA 384 ||| ||||||| ||||||||||||| |||| |||||||||||| | | |||||||| Sbjct 2519 CGCAAAGAAATACTGCGGCTTATGCGTTAC---TAACATTGCACAGGTTTA-AGACTGCA 2574 Query 385 AATGGGAAAGCATTCATGCGTAAACAAGAGCTTATGGACGCTGCTGACGCTAGTGGGCTC 444 ||||||||| ||| |||||||||||| |||||||||||||||||||||||||||||||| Sbjct 2575 AATGGGAAAAAATTGATGCGTAAACAAAAGCTTATGGACGCTGCTGACGCTAGTGGGCTC 2634 Query 445 TCACACGTTCCTATTGCACCAGAGAAAGGTAAAGGAAAAGTAGGAGTTGGAAACTCTAAG 504 ||||| ||| ||||||| ||||||||||| |||||||||| |||| |||||| ||||||| Sbjct 2635 TCACATGTTTCTATTGCGCCAGAGAAAGGAAAAGGAAAAGCAGGACTTGGAACCTCTAAG 2694 Query 505 AGGGAGTGGTATAGCGGGTGGAGCTGCATGAAGACACTTATAGAGAAGGGATTGGTGGTG 564 ||||||||||||||||| |||||||||||| || ||||||||||||||||||||||||| Sbjct 2695 AGGGAGTGGTATAGCGGATGGAGCTGCATGTCGAAACTTATAGAGAAGGGATTGGTGGTG 2754 Query 565 AAATCTAGCAACCCGGCAAAGTACATGCTGACAGTTGAAGGTCGGAATGTAGCTGATGAC 624 |||| ||||||||| |||||||||||||||||||||||||| ||||||||||| |||||| Sbjct 2755 AAATTTAGCAACCCAGCAAAGTACATGCTGACAGTTGAAGGCCGGAATGTAGCAGATGAC 2814 Query 625 T 625 | Sbjct 2815 T 2815 Score = 353 bits (191), Expect = 3e-96 Identities = 287/329 (87%), Gaps = 24/329 (7%) Strand=Plus/Plus Query 763 GATGCAAGATCCAGGGGACAATCATCCATTCCAGCTGGACCTTCTTCTAGAGCACAAGCA 822 ||| ||||||||||||||||||||||||| ||||| |||||||||||||||||||||| | Sbjct 2818 GATCCAAGATCCAGGGGACAATCATCCATCCCAGCAGGACCTTCTTCTAGAGCACAAGAA 2877 Query 823 TTCTTAGCTACAAATACAGGTCAAGTTGATCTTGAGGGATCTTTC-T-CTAAGAAATTCC 880 |||||||||||| |||||||||||||||||||||||||||| || | ||||||| |||| Sbjct 2878 TTCTTAGCTACAGATACAGGTCAAGTTGATCTTGAGGGATC--TCGTGCTAAGAAGTTCC 2935 Query 881 GTTCTTGCAATGTTGGATCCACCTTAAATCCATGTTCTTCCGGT-TCGAGCCATGCCATG 939 |||||| ||| | |||||||||||| || | ||||||||| || Sbjct 2936 GTTCTT-CAA-----G------------TCCATGTTCTTCTAGTAT-AAGCCATGCCTTG 2976 Query 940 AAAGCCTGCTCATCTTCCTTGGCGTCCGATGGAACCGAAGGAGTGACAACTATTACACGC 999 ||||||||||||||||| ||||| || ||||||||||||||||| ||||||||| ||||| Sbjct 2977 AAAGCCTGCTCATCTTCTTTGGCATCTGATGGAACCGAAGGAGTAACAACTATTCCACGC 3036 Query 1000 CTTCCACCTCTTAGATTCGGAGAAGCGTTTGAGGAAGCATATGATGTGATCTTGTTACTG 1059 ||||||||||||||||||||||||||||||||||||| |||||||||||||||| ||||| Sbjct 3037 CTTCCACCTCTTAGATTCGGAGAAGCGTTTGAGGAAGTATATGATGTGATCTTGATACTG 3096 Query 1060 GATGATCGAGAACAGTTTGCCACCAAGGG 1088 ||||||||||||||||||||||| ||||| Sbjct 3097 GATGATCGAGAACAGTTTGCCACTAAGGG 3125 Score = 250 bits (135), Expect = 4e-65 Identities = 147/152 (97%), Gaps = 3/152 (2%) Strand=Plus/Plus Query 1634 TTGCCATAGCTGTTCTTGATAAGTACAAAACACTTCGGTCTCTTGCTTCTGCCTATTCTA 1693 |||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| Sbjct 3611 TTGCCATAGCTGTTCTTGATAAGTACAAAACACTTCGGTCTCTTGCTTCTGCGTATTCTA 3670 Query 1694 AACTAGATGGGAATGTCTCGGCGCAAGAAGAAATGCTGCGGAATCAAAGCAACAATGTAA 1753 |||||||| ||||||||||||||||||||||||||| ||||||||||||||||||||| Sbjct 3671 AACTAGAT---AATGTCTCGGCGCAAGAAGAAATGCTGAGGAATCAAAGCAACAATGTAA 3727 Query 1754 TATGTGCATCAGCTAGTAAGAATATATACAAG 1785 |||||||||||||||||||||||||||||||| Sbjct 3728 TATGTGCATCAGCTAGTAAGAATATATACAAG 3759 > AT5G41940.1 | Symbols: | Ypt/Rab-GAP domain of gyp1p superfamily protein | chr5:16781947-16785576 FORWARD LENGTH=1867 Length=1867 Score = 178 bits (96), Expect = 2e-43 Identities = 149/173 (86%), Gaps = 9/173 (5%) Strand=Plus/Plus Query 1785 GACGGAATATGAAAACATGCGGAGACAGTGTCGCGAGATTCATGAACGCAATGAAAATGG 1844 |||||||||||||||| |||||||||||||||||||||||||||||||| ||||||||| Sbjct 551 GACGGAATATGAAAACCTGCGGAGACAGTGTCGCGAGATTCATGAACGCTATGAAAATGT 610 Query 1845 CTGTGACTCAAAGCAGA--GC--AG--C-G-A-CACTGAAGACATTCAGGTTCTTGATTC 1895 ||| ||||||||| ||| || || | | | ||||||||||| |||||||||||| || Sbjct 611 CTGCGACTCAAAGGAGACAGCTCAGAGCAGCAACACTGAAGACAGTCAGGTTCTTGAATC 670 Query 1896 CCATAATATTGAGGAGGTCGATAGTTCTACAAGATCTGTCACCGTAGAAGAAT 1948 |||| |||||||||| ||||| ||||| |||||| | |||||||||| |||| Sbjct 671 CCATGATATTGAGGAAGTCGAGAGTTCCACAAGAACCATCACCGTAGATGAAT 723 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 102760515615 Database: TAIR10_cdna_20110103_representative_gene_model_updated Posted date: Sep 25, 2014 6:13 PM Number of letters in database: 51,074,197 Number of sequences in database: 33,602 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5