BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: halleri_transcript_ahg.fa 32,672 sequences; 38,939,717 total letters Query= AT1G53640.1 Length=873 Score E Sequences producing significant alignments: (Bits) Value Ahg314680 jgi|Araly1|314680|fgenesh1_pm.C_scaffold_1003460 529 2e-149 Ahg914555 jgi|Araly1|914555|Al_scaffold_0001_4809 167 1e-40 > Ahg314680 jgi|Araly1|314680|fgenesh1_pm.C_scaffold_1003460 Length=669 Score = 529 bits (286), Expect = 2e-149 Identities = 469/554 (85%), Gaps = 25/554 (5%) Strand=Plus/Plus Query 1 ATGAAATTCGATGATAAGTACAAACTTATTGTTGTAGGCATCAGCGGCGTTTTAGCGGGA 60 ||||||||||||||||| ||||||||||||||| ||| |||| ||||||||||||| ||| Sbjct 1 ATGAAATTCGATGATAACTACAAACTTATTGTTATAGTCATCGGCGGCGTTTTAGCTGGA 60 Query 61 GTTGTTGTCATAATGTTTATCTGTTGTTACAAGAACAAGAAGAAGAAGTTTCAGCTACCG 120 |||| |||| | |||||| || |||||||||||||||| |||||| ||||||||||| Sbjct 61 GTTGCTGTCGTTATGTTT-TC--CTGTTACAAGAACAAGAGGAAGAACCTTCAGCTACCG 117 Query 121 GAGCCGCCACAGACACCGGAGTGGCAGCGACAAATTGTTTCTGCTCCGACACTTCAGACA 180 |||| | |||||||||||||||||||||||||||||||||||||||| |||||||||||| Sbjct 118 GAGCTGACACAGACACCGGAGTGGCAGCGACAAATTGTTTCTGCTCCTACACTTCAGACA 177 Query 181 AGAAACATTGATGTCGGAAATGATCGCTTGTGGGGTAAAAGCGAAACCGTTGTGAAGAAA 240 ||||||||||||||||||||||||||||||||||||| |||||| ||||||||||| ||| Sbjct 178 AGAAACATTGATGTCGGAAATGATCGCTTGTGGGGTAGAAGCGACACCGTTGTGAATAAA 237 Query 241 TCTGTCATTCCACTGAGGCCAAACGCAGT-AGACAATACCTTGCGCTATCAGGGGCCTAA 299 ||| | |||||||| | ||||||||| | | || ||| |||||| || ||| ||||||| Sbjct 238 TCTATTATTCCACTTAAGCCAAACGCTTTCA-ACGATAGCTTGCGATACCAGAGGCCTAA 296 Query 300 AAATTTGGAGAGGCCTCGGAGACAGAGAGATGATGTGACGCCTGCAGCTTCTACTTCAGC 359 ||||||||||||||||||| ||||||||||||||| ||| ||| |||||||||||||| Sbjct 297 AAATTTGGAGAGGCCTCGGGGACAGAGAGATGATGGGAC---TGCGGCTTCTACTTCAGC 353 Query 360 ATTTGCTGGTGATTGTGGTGGCAGTAGCGGTGGTGACAACGGTTGTGATGGTGGTCACAG 419 ||||| ||||| ||||| || || ||||||||||||||||||||||| || | |||| || Sbjct 354 ATTTGGTGGTGGTTGTGTTGCCATTAGCGGTGGTGACAACGGTTGTGGTGCTAGTCATAG 413 Query 420 TGGCGGTGCTGTGACTGAGTTTTGCTA-TACGATCTCGATGGTTCGT-ATGTTTTGTGGA 477 ||| |||| || || ||| || | | ||| | | |||| | | || | | | Sbjct 414 TGGTGGTG--GTCAC--AGTGGTGGTGGTG-GATGTGG-TGGTGGGGGAGGTG--GAG-A 464 Query 478 ATCCTCAGCCGGAAGAATAAAAAGCCTTCGTCGTCTCCGTCTTCACCGTCGCGAGATATT 537 || |||||||||| ||||||||||| |||||| |||||||||||||||||||||| Sbjct 465 AT-----GCCGGAAGAAGAAAAAGCCTTCATCGTCTTCGTCTTCACCGTCGCGAGATATC 519 Query 538 AAAAAGG-TAAGAC 550 ||||||| |||||| Sbjct 520 AAAAAGGGTAAGAC 533 > Ahg914555 jgi|Araly1|914555|Al_scaffold_0001_4809 Length=255 Score = 167 bits (90), Expect = 1e-40 Identities = 123/139 (88%), Gaps = 2/139 (1%) Strand=Plus/Plus Query 675 CAAGGAGGAGGAGGCGAAGTCATCTCCTCCAAAAGATAACGTCAAAAAAGGTGAAACGAC 734 ||||||||||||| ||| |||||| ||||| | |||| | | ||||||||| || ||| | Sbjct 30 CAAGGAGGAGGAGCCGATGTCATCACCTCCGAGAGATGATGCCAAAAAAGGAGAGACGGC 89 Query 735 GGGaaaaaaaGATGGAGGTCTTGTTGTT-GTGTCGTCTTCGGAAAAAAGTAAGACCAAAG 793 ||||| ||||||||||||||||||| || |||||||||||| ||||||||||||||||| Sbjct 90 GGGAACAAAAGATGGAGGTCTTGTT-TTCATGTCGTCTTCGGGAAAAAGTAAGACCAAAG 148 Query 794 GCTGTTGTTGCGGCGGAgg 812 ||||||||||||||||||| Sbjct 149 GCTGTTGTTGCGGCGGAGG 167 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 32328233616 Database: halleri_transcript_ahg.fa Posted date: Sep 25, 2014 2:02 AM Number of letters in database: 38,939,717 Number of sequences in database: 32,672 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5