BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT1G53815.1
Length=714
Score E
Sequences producing significant alignments: (Bits) Value
Ahg337517 jgi|Araly1|337517|fgenesh1_pg.C_scaffold_1004082 320 8e-87
Ahg315251 jgi|Araly1|315251|fgenesh1_pm.C_scaffold_2000284 265 4e-70
Ahg337498 jgi|Araly1|337498|fgenesh1_pg.C_scaffold_1004063 80.5 2e-14
> Ahg337517 jgi|Araly1|337517|fgenesh1_pg.C_scaffold_1004082
Length=918
Score = 320 bits (173), Expect = 8e-87
Identities = 483/624 (77%), Gaps = 56/624 (9%)
Strand=Plus/Plus
Query 50 GATCCAATAAACCG-TCTTCAACACCTGGA-A--AAGACATAGAATACTTTGGTCTGA-T 104
||||||||||| || ||||||||| ||||| | || |||| | ||||||| || || |
Sbjct 50 GATCCAATAAA-CGATCTTCAACATCTGGAGAGGAA-ACAT-G-TTACTTTGATCCGATT 105
Query 105 CCCTGTTGATCTCTTCATCAGTAATATCTTATCGAGATTGCCGCTGAAGTCTAAAGCTAA 164
||| ||||||||| ||||| |||||||| ||||||||||| ||| ||| || || |
Sbjct 106 CCC-GTTGATCTCATCATC---AATATCTTGTCGAGATTGCCTCTGGAGTGTATTGCGAG 161
Query 165 GTGTCGCTGCGTATCGAAGCTCTGGTCGTCCATAATTCGCCGTCCAAATTACAACCAGTT 224
|||||| || ||||| || || |||||||||||| ||||||||||||||||||||| |||
Sbjct 162 GTGTCGTTGTGTATCCAAACTGTGGTCGTCCATAGTTCGCCGTCCAAATTACAACCTGTT 221
Query 225 GTTCCCGATCAAGTCCCCAGCT---CCGCGGATCCTTTTCACCATCGAA-TATGCAGGAA 280
||||||| ||||||| || ||| |||||| |||||||| | || || | | || |||
Sbjct 222 GTTCCCGTTCAAGTCTCCGGCTACACCGCGGCTCCTTTTCGCGTTCAAAGT-TTCA-GAA 279
Query 281 -TCTTGTTCTTTTACTCTTCACCTCAGCCTCACAATCCGGATG-A-GAACTCGTCTCTTG 337
||||| || ||||||||||||||||||||||||| | || | ||||| ||||||||
Sbjct 280 GAGTTGTTATTCAACTCTTCACCTCAGCCTCACAATCC-G-TGTAGGAACTTGTCTCTTG 337
Query 338 TAGCCACCCT-TCATCAGAACACTGGTAACACCAAGTTCTATGAGATATGTGGTCCTGTC 396
|||||| ||| || ||||| ||| || | | || |||| | || | ||| | ||||||
Sbjct 338 TAGCCA-CCTCTCTTCAGAGGACTTCTAGCGCAAATTTCTTTAAGTTTTGTCGCCCTGTC 396
Query 397 TGCGGACGGGTACTGT-G---TC--GTC-AGTAT-A--ATTCAGTTGG-GGTGATTTATA 445
|||| ||| |||| | | || | || | |||||| ||| ||| |||||||
Sbjct 397 CACGGATTGGT-CTGTCGCCAACATATCGAAAATAACTATTCAG-TGGCGGTAATTTATA 454
Query 446 ATCCCATCACAGGAGAGTC-TTCAAAC-TTGCCGAAACTAAGTTTAAAGGGGATTAA-TA 502
||||||||||||||||||| ||| || || || ||||| || | ||||||| || |
Sbjct 455 ATCCCATCACAGGAGAGTCTTTC--ACTTTACCCAAACTGAGAATGGAGGGGATGAATTC 512
Query 503 AGTCAGAGTG-GTTATGTGATAAAGCAAGATATTCTTTTGGGTATGATCCAATCGAGAAA 561
|| |||| | | ||| || |||| |||||||||||||||||||||||| ||||| |||
Sbjct 513 AG--AGAG-GAGAAATG-GA-AAAGTAAGATATTCTTTTGGGTATGATCCGATCGAAAAA 567
Query 562 CAGCTCAAAGTGTTGTGTATCACCTGGTTGCGTA-CTGGCTCAGA-GCA-CTTGTCCAAT 618
|| |||| || ||| ||||||| |||||||||| | |||||| | | | | ||| | |
Sbjct 568 CAATTCAAGGTATTGCGTATCACATGGTTGCGTAGC-GGCTCACACG-AGCG-GTCTAGT 624
Query 619 GAGTATCAAGTTCTCACATTAGGG 642
|||||||||||||| || ||||||
Sbjct 625 GAGTATCAAGTTCTAACGTTAGGG 648
> Ahg315251 jgi|Araly1|315251|fgenesh1_pm.C_scaffold_2000284
Length=759
Score = 265 bits (143), Expect = 4e-70
Identities = 237/281 (84%), Gaps = 11/281 (4%)
Strand=Plus/Plus
Query 80 AAGACATAGAATACTTTGGT-CTGATCCCTGTTGATCTCTTCATC-AGTAATATCTTATC 137
|||| | |||||| |||| | | ||||| |||||||||||||| | |||| |||||| ||
Sbjct 23 AAGAAACAGAATATTTTGATGC-GATCCATGTTGATCTCTTCA-CTAGTACTATCTTGTC 80
Query 138 GAGATTGCCGCTGAAGTCTAAAGCTAAGTGTCGCTGCGTATCGAAGCTCTGGTCGTCCAT 197
|| ||||||| | |||||| |||| ||||||| |||||||| |||||||||||||||||
Sbjct 81 GAAATTGCCGGTAAAGTCTTTAGCTCAGTGTCGTTGCGTATCCAAGCTCTGGTCGTCCAT 140
Query 198 AATTCGCCGTCCAAATTACAACCA-GTTGTTCCCGATCAAGTCCCCAGCTCC---GCGGA 253
||||||||||||| |||||||| | ||||||||| |||||||| || | ||| |||||
Sbjct 141 AATTCGCCGTCCATATTACAAC-ATGTTGTTCCCCATCAAGTCTCCGGATCCACCGCGGA 199
Query 254 TCCTTTTCACCATCG-AATATGCAGGAATCTTGTTCTTTTACTCTTCACCTCAGCCTCAC 312
|||||| | ||||| || |||||||| |||||||| ||||||||||||||||||
Sbjct 200 TCCTTTGGAGCATCGGAA-ATGCAGGAGGGTTGTTCTTCTACTCTTCACCTCAGCCTTGT 258
Query 313 AATCCGGATGAGAACTCGTCTCTTGTAGCCACCCTTCATCA 353
|| |||||||||||| |||||||||||||||| ||||||||
Sbjct 259 AACCCGGATGAGAACACGTCTCTTGTAGCCACACTTCATCA 299
Score = 78.7 bits (42), Expect = 5e-14
Identities = 112/144 (78%), Gaps = 11/144 (8%)
Strand=Plus/Plus
Query 432 TGG-GGTGATTTATAATCCCATCACAGGAGAGTCTTCAAACTTGCCGAAACTAAGT-TTA 489
||| ||| |||| ||||||||| || ||||||| || | |||||| ||||| | | ||
Sbjct 389 TGGAGGTAATTTCTAATCCCATAACGGGAGAGTTTTTAGCCTTGCCCAAACTGA-TAATA 447
Query 490 AA-GGGGATTAATAAGTCAGAGTGGTTATGTGAT-AAAGCAAGATATTCTTTTGGGTATG 547
|| | || |||| || ||||||||||| || | | | || || || |||||||||||
Sbjct 448 AATGAGG-TTAAGAA---AGAGTGGTTATTTG-TCCATGAAACATTTTTTTTTGGGTATG 502
Query 548 ATCCAATCGAGAAACAGCTCAAAG 571
||||||||||||||||| || |||
Sbjct 503 ATCCAATCGAGAAACAG-TCCAAG 525
> Ahg337498 jgi|Araly1|337498|fgenesh1_pg.C_scaffold_1004063
Length=1488
Score = 80.5 bits (43), Expect = 2e-14
Identities = 49/52 (94%), Gaps = 0/52 (0%)
Strand=Plus/Plus
Query 295 TCTTCACCTCAGCCTCACAATCCGGATGAGAACTCGTCTCTTGTAGCCACCC 346
||||||||||| ||||| ||||| ||||||||||||||||||||||||||||
Sbjct 274 TCTTCACCTCAACCTCAAAATCCAGATGAGAACTCGTCTCTTGTAGCCACCC 325
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 26266689813
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5