BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT1G55460.1
Length=1547
Score E
Sequences producing significant alignments: (Bits) Value
Ahg474601 jgi|Araly1|474601|fgenesh2_kg.1__4555__AT1G55460.1 1908 0.0
Ahg497310 jgi|Araly1|497310|fgenesh2_kg.185__1__AT1G55460.1 1066 0.0
Ahg924054 jgi|Araly1|924054|scaffold_105335.1 817 0.0
Ahg914671 jgi|Araly1|914671|Al_scaffold_0001_4925 577 1e-163
> Ahg474601 jgi|Araly1|474601|fgenesh2_kg.1__4555__AT1G55460.1
Length=1242
Score = 1908 bits (1033), Expect = 0.0
Identities = 1174/1243 (94%), Gaps = 5/1243 (0%)
Strand=Plus/Plus
Query 87 TCGATGGGTAAGAACGATTTTCTAACCCCAAAGGCGATTGCGAATCGAATTAAAGCCAAA 146
||||||||||||||||||||||||||||| ||||| |||||||||||||| |||||||||
Sbjct 1 TCGATGGGTAAGAACGATTTTCTAACCCCGAAGGCAATTGCGAATCGAATCAAAGCCAAA 60
Query 147 GGACTTCAAAAGCTTCGATGGTATTGTCAGATGTGTCAGAAACAATGCCGAGACGAGAAT 206
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 61 GGACTTCAAAAGCTTCGATGGTATTGTCAGATGTGTCAGAAGCAATGCCGAGACGAGAAT 120
Query 207 GGATTCAAGTGTCATTGTATGAGCGAATCTCACCAGAGGCAGATGCAAGTTTTTGGGCAG 266
|||||||||||||| ||||||||||||||||||||||||||||||||||| ||||| |||
Sbjct 121 GGATTCAAGTGTCACTGTATGAGCGAATCTCACCAGAGGCAGATGCAAGTCTTTGGTCAG 180
Query 267 AATCCGACTCGTGTTGTCGATGGTTACTCTGAAGAATTCGAGCAGACGTTTCTCGATCTG 326
||||||||||||||||| ||||||||||| ||||| ||||||||||||||||||||||||
Sbjct 181 AATCCGACTCGTGTTGTTGATGGTTACTCCGAAGAGTTCGAGCAGACGTTTCTCGATCTG 240
Query 327 ATGAGGCGGAGCCACCGTTTCTCTCGTATCGCTGCCACTGTTGTCTACAATGAGTATATT 386
||| ||||||||||||| |||||||||||||| ||||||||||| ||||||||||||||
Sbjct 241 ATGCGGCGGAGCCACCGCTTCTCTCGTATCGCCGCCACTGTTGTTTACAATGAGTATATC 300
Query 387 AACGATAGACATCATGTTCACATGAATTCGACGGAGTGGGCGACGTTGACGGAGTTTATC 446
|||||||| ||||||||||| |||||||| |||||||||||||| ||||| |||||||||
Sbjct 301 AACGATAGGCATCATGTTCATATGAATTCCACGGAGTGGGCGACTTTGACTGAGTTTATC 360
Query 447 AAGCATCTTGGGAAAACTGGTAAGTGTAAGGTTGAAGAAACTCCTAAAGGTTGGTTTATT 506
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 361 AAGCATCTTGGGAAAACTGGTAAGTGTAAGGTTGAAGAAACTCCTAAAGGTTGGTTTATT 420
Query 507 ACTTATATAGATAGAGATTCTGAGACTTTGTTTAAGGAGAGGTTGAAGAATAAGAGAGTT 566
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 421 ACTTATATAGATAGAGATTCTGAGACTTTGTTTAAGGAGAGGTTGAAAAATAAGAGAGTT 480
Query 567 AAGAGTGATTTAGCTgaggaggagaagcaagagagggagattcaga-gacagattgagag 625
|||||||||||||||||||||||||||||||||||||||||||||| | |||||||||||
Sbjct 481 AAGAGTGATTTAGCTGAGGAGGAGAAGCAAGAGAGGGAGATTCAGAAG-CAGATTGAGAG 539
Query 626 agctgctgagaAATtgaatggtggtggtggtgaaggtgaaaccagtggtaatgatgaagt 685
|||||||||||| | ||||| || ||||||||||||||||| ||||||||| || | ||
Sbjct 540 AGCTGCTGAGAAGTCGAATGCTGTTGGTGGTGAAGGTGAAAGCAGTGGTAAAGAAGCTGT 599
Query 686 tgtggatgatggtgatgatga-GAG--GAAGAAAGATGAAGACTTGAGGTTGAAGAGTGG 742
| |||||||| ||||||||| | | ||||||||||||||| |||||||| ||||||||
Sbjct 600 GGAGGATGATGATGATGATGATGTGAAGAAGAAAGATGAAGAATTGAGGTTTAAGAGTGG 659
Query 743 GGTCAAGGTTGGATTTGCTCTTGGTGGAGGTGTTAAACAGGTTGCTACGGGTAAAGAGAG 802
||| || |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 660 GGTTAAAGTTGGATTTGCTCTTGGTGGAGGTGTTAAACAGGTTGCTACGGGTAAAGAGAG 719
Query 803 AGGGGAAAGCTCGAAACTTTTGTTTGGGGATGAAGAGAATGACAAGGTTgagagagggga 862
||||||||||||||||||| |||||||||||||||||||||| ||||||| |||||||||
Sbjct 720 AGGGGAAAGCTCGAAACTTGTGTTTGGGGATGAAGAGAATGAGAAGGTTGGGAGAGGGGA 779
Query 863 gaagaggaagagaagtggggactccgggaggagcgagaaggagaggagatcagctttgga 922
|||||||||||||| ||||| |||||||||||||||||||| || ||||||||||||||
Sbjct 780 TAAGAGGAAGAGAAGCGGGGAATCCGGGAGGAGCGAGAAGGACAGAAGATCAGCTTTGGA 839
Query 923 tgagttgatgaaggaggaagagaagaagaaggagaggatgaaTCGGAAAGATTACTGGTT 982
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 840 TGAGTTGATGAAGGAGGAAGAGAAGAAGAAGGAGAGGATGAATCGGAAAGACTACTGGTT 899
Query 983 GTTCGAAGGGATCATTGTGAAGGTGATGAGTAAGGCTTTGGCAGAGAAAGGGTATTATAA 1042
|||||| ||||| ||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct 900 GTTCGAGGGGATAATTGTGAAGGTAATGAGTAAGGCTTTGGCAGAGAAAGGGTATTATAA 959
Query 1043 GCAAAAAGGTGTTGTGAAGAAAGTGATTGATAATTATGTTGGGGAGATCAAAATGCTTGA 1102
|||||| |||||||||||||||||||||||||| ||||| ||||||||| ||||||||||
Sbjct 960 GCAAAAGGGTGTTGTGAAGAAAGTGATTGATAAGTATGTGGGGGAGATCGAAATGCTTGA 1019
Query 1103 TTCTAAACATGTGTTGAGAGTTGATCAAAAGGAGCTAGAGACTGTGCTTCCACAAATTGG 1162
|||||| |||||| |||||||||||||| |||||| ||||||||||||||||| || ||
Sbjct 1020 TTCTAAGCATGTGCTGAGAGTTGATCAAGTGGAGCTCGAGACTGTGCTTCCACAGATAGG 1079
Query 1163 AGGGATGGTGAAGATAGTGAATGGGGCATATCGTGGTTCGAATGCAAGGTTGTTGGGTGT 1222
|||||| |||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 1080 AGGGATAGTGAAGATAGTGAATGGGGCATATCGTGGTTCGAATGCAAAGTTGTTGGGTGT 1139
Query 1223 GGATACAGAGAAGTTCTGTGCTAAAGTGCAGATCGAGAAAGGTGTCTATGATGGAAGAGT 1282
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 1140 GGATACAGAGAAGTTCTGTGCTAAAGTGCAAATCGAGAAAGGTGTCTATGATGGAAGAGT 1199
Query 1283 CATCAAGTCCATTGAGTACGAGGACATATGCAAACTTGCTTAG 1325
|||||||||||||||||||||||||||||||||||||||||||
Sbjct 1200 CATCAAGTCCATTGAGTACGAGGACATATGCAAACTTGCTTAG 1242
> Ahg497310 jgi|Araly1|497310|fgenesh2_kg.185__1__AT1G55460.1
Length=1290
Score = 1066 bits (577), Expect = 0.0
Identities = 694/750 (93%), Gaps = 9/750 (1%)
Strand=Plus/Plus
Query 589 agaagcaag-agagggagattcagagacagattgagagagctgctgagaAATtgaatggt 647
|||| |||| ||||||||||||||| |||||||||||||||||||||||||||||||| |
Sbjct 547 AGAA-CAAGAAGAGGGAGATTCAGAAACAGATTGAGAGAGCTGCTGAGAAATTGAATGCT 605
Query 648 ggtggtggtgaaggtgaaaccagtggtaatgatgaagttgtggatg-atggtgatgatga 706
|||||||||||||||||| |||||||| | ||||||||||| | || ||||||| |
Sbjct 606 CGTGGTGGTGAAGGTGAAAGTAGTGGTAA--A-GAAGTTGTGGAGGAAT-ATGATGATAA 661
Query 707 GAGGAAGAAAGATGAAGACTTGAGGTTGAAGAGTGGGGTCAAGGTTGGATTTGCTCTTGG 766
|| ||||||||| ||||| |||||||||||||||||||| ||||||||||||||||||||
Sbjct 662 GAAGAAGAAAGAGGAAGAATTGAGGTTGAAGAGTGGGGTTAAGGTTGGATTTGCTCTTGG 721
Query 767 TGGAGGTGTTAAACAGGTTGCTACGGGTAAAGAGAGAGGGGAAAGCTCGAAACTTTTGTT 826
||||||||||||||||||| |||||||||||||||||| |||||||||||||||| | ||
Sbjct 722 TGGAGGTGTTAAACAGGTTACTACGGGTAAAGAGAGAGTGGAAAGCTCGAAACTTGTCTT 781
Query 827 TGGGGATGAAGAGAATGACAAGGTTgagagaggggagaagaggaagagaagtggggactc 886
||||| |||||||||||| |||||| ||||||| ||||||||||||||||| ||||||||
Sbjct 782 TGGGGTTGAAGAGAATGAGAAGGTTCAGAGAGGTGAGAAGAGGAAGAGAAGCGGGGACTC 841
Query 887 cgggaggagcgagaaggagaggagatcagctttggatgagttgatgaaggaggaagagaa 946
|||||||||| ||| ||| | ||||||||||||||||||||||||||||||||||||||
Sbjct 842 CGGGAGGAGCAAGACGGATAAAAGATCAGCTTTGGATGAGTTGATGAAGGAGGAAGAGAA 901
Query 947 gaagaaggagaggatgaaTCGGAAAGATTACTGGTTGTTCGAAGGGATCATTGTGAAGGT 1006
||||||||||||||||||||||||||||||||||||||| ||||| ||||||||||||||
Sbjct 902 GAAGAAGGAGAGGATGAATCGGAAAGATTACTGGTTGTTTGAAGGAATCATTGTGAAGGT 961
Query 1007 GATGAGTAAGGCTTTGGCAGAGAAAGGGTATTATAAGCAAAAAGGTGTTGTGAAGAAAGT 1066
||||||||||||||||||| |||||||||||||||||||||| |||||||||||||||||
Sbjct 962 GATGAGTAAGGCTTTGGCACAGAAAGGGTATTATAAGCAAAAGGGTGTTGTGAAGAAAGT 1021
Query 1067 GATTGATAATTATGTTGGGGAGATCAAAATGCTTGATTCTAAACATGTGTTGAGAGTTGA 1126
| ||||||| ||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 1022 GGTTGATAAGTATGTTGGGGAGATCGAAATGCTTGATTCTAAACATGTGTTGAGAGTTGA 1081
Query 1127 TCAAAAGGAGCTAGAGACTGTGCTTCCACAAATTGGAGGGATGGTGAAGATAGTGAATGG 1186
||| ||||||||||||||||||||||||||| |||||||| |||||||||||||||||
Sbjct 1082 TCAGGTGGAGCTAGAGACTGTGCTTCCACAAATAGGAGGGATAGTGAAGATAGTGAATGG 1141
Query 1187 GGCATATCGTGGTTCGAATGCAAGGTTGTTGGGTGTGGATACAGAGAAGTTCTGTGCTAA 1246
|||||||||| | ||||||||||||||||||||| |||||||||| |||||||| |||||
Sbjct 1142 GGCATATCGTCGCTCGAATGCAAGGTTGTTGGGTTTGGATACAGAAAAGTTCTGCGCTAA 1201
Query 1247 AGTGCAGATCGAGAAAGGTGTCTATGATGGAAGAGTCATCA-AGTCCATTGAGTACGAGG 1305
|||||| ||||||||| | |||||||||||||||||||||| | ||||| ||||||||||
Sbjct 1202 AGTGCAAATCGAGAAAAGCGTCTATGATGGAAGAGTCATCAGA-TCCATCGAGTACGAGG 1260
Query 1306 ACATATGCAAACTTGCTTAGTTATATCTTT 1335
||||||||||||||||||||||||||||||
Sbjct 1261 ACATATGCAAACTTGCTTAGTTATATCTTT 1290
Score = 869 bits (470), Expect = 0.0
Identities = 529/558 (95%), Gaps = 1/558 (0%)
Strand=Plus/Plus
Query 8 TCGATCAGTGGTTGTTACCTGAGAAACCCTAGATTTCAATTCCCAACACCTTAATTTTGT 67
||||| ||||||||||||| |||||||||||||||||||||| ||| ||||| |||||||
Sbjct 2 TCGATTAGTGGTTGTTACCGGAGAAACCCTAGATTTCAATTCGCAAGACCTTGATTTTGT 61
Query 68 TCGACAAACCCAAGAAGATTCGATGGGTAAGAACGATTTTCTAACCCCAAAGGCGATTGC 127
|| ||||||||| ||||| ||||||||||||||||||||||||||||| |||||||||||
Sbjct 62 TCTACAAACCCACGAAGAATCGATGGGTAAGAACGATTTTCTAACCCCGAAGGCGATTGC 121
Query 128 GAATCGAATTAAAGCCAAAGGACTTCAAAAGCTTCGATGGTATTGTCAGATGTGTCAGAA 187
||||| ||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 122 GAATCAAATCAAAGCCAAAGGACTTCAAAAGCTTCGATGGTATTGTCAGATGTGTCAGAA 181
Query 188 ACAATGCCGAGACGAGAATGGATTCAAGTGTCATTGTATGAGCGAATCTCACCAGAGGCA 247
||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 182 GCAATGCCGAGATGAGAATGGATTCAAGTGTCATTGTATGAGCGAATCTCACCAGAGGCA 241
Query 248 GATGCAAGTTTTTGGGCAGAATCCGACTCGTGTTGTCGATGGTTACTCTGAAGAATTCGA 307
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct 242 GATGCAAGTTTTTGGGCAGAATCCAACTCGTGTTGTCGATGGTTACTCTGAAGAATTCGA 301
Query 308 GCAGACGTTTCTCGATCTGATGAGGCGGAGCCACCGTTTCTCTCGTATCGCTGCCACTGT 367
|||||||||||||||||||||| ||||||||||||| |||||||||||||| ||||||||
Sbjct 302 GCAGACGTTTCTCGATCTGATGCGGCGGAGCCACCGCTTCTCTCGTATCGCCGCCACTGT 361
Query 368 TGTCTACAATGAGTATATTAACGATAGACATCATGTTCACATGAATTCGACGGAGTGGGC 427
||| |||||||||||||| |||||||| ||||||||||| |||||||| |||||||||||
Sbjct 362 TGTTTACAATGAGTATATCAACGATAGGCATCATGTTCATATGAATTCCACGGAGTGGGC 421
Query 428 GACGTTGACGGAGTTTATCAAGCATCTTGGGAAAACTGGTAAGTGTAAGGTTGAAGAAAC 487
||||||||||||||| |||||| | ||||||||||| |||||||||||||||||||||||
Sbjct 422 GACGTTGACGGAGTTCATCAAGTACCTTGGGAAAACAGGTAAGTGTAAGGTTGAAGAAAC 481
Query 488 TCCTAAAGGTTGGTTTATTACTTATATAGATAGAGATTCTGAGACTTTGTTTAAGGAGAG 547
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 482 TCCTAAAGGTTGGTTTATTACTTATATAGATAGAGATTCGGAGACTTTGTTTAAGGAGAG 541
Query 548 GTTGAAGAATAAGA-GAG 564
||||||||| |||| |||
Sbjct 542 GTTGAAGAACAAGAAGAG 559
> Ahg924054 jgi|Araly1|924054|scaffold_105335.1
Length=580
Score = 817 bits (442), Expect = 0.0
Identities = 534/575 (93%), Gaps = 19/575 (3%)
Strand=Plus/Plus
Query 185 GAAACAATGCCGAGACGAGAATGGATTCAAGTGTCATTGTATGAGCGAATCTCACCAGAG 244
|||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 1 GAAACAATGCCGAGACGAGAATGGATTCAAGTGT--TTGTATGAGCGAATCTCACCAGAG 58
Query 245 GCAGATGCAAGTTTTTGGGCAGAATCCGACTCGTGTTGTCGATGGTTACTCTGAAGAATT 304
|||||||||||||||||| |||||||||||||||||||| ||||||||||||||||| ||
Sbjct 59 GCAGATGCAAGTTTTTGGCCAGAATCCGACTCGTGTTGTTGATGGTTACTCTGAAGAGTT 118
Query 305 CGAGCAGACGTTTCTCGATCTGATGAGGCGGAGCCACCGTTTCTCTCGTATCGCTGCCAC 364
||||||||||||||||||||||||| ||||||||||||| |||||||||||||| |||||
Sbjct 119 CGAGCAGACGTTTCTCGATCTGATGCGGCGGAGCCACCGCTTCTCTCGTATCGCCGCCAC 178
Query 365 TGTTGTCTACAATGAGTATATTAACGATAGACATCATGTTCACATGAATTCGACGGAGTG 424
|||||| |||||||||||||||||||||||||||||||| || |||||||| ||||||||
Sbjct 179 TGTTGTTTACAATGAGTATATTAACGATAGACATCATGTCCATATGAATTCCACGGAGTG 238
Query 425 GGCGACGTTGACGGAGTTTATCAAGCATCTTGGGAAAACTGGTAAGTGTAAGGTTGAAGA 484
|||||||||||||||||| || ||||||||||||||||||||||||||||||||||| ||
Sbjct 239 GGCGACGTTGACGGAGTTCATAAAGCATCTTGGGAAAACTGGTAAGTGTAAGGTTGAGGA 298
Query 485 AACTCCTAAAGGTTGGTTTATTACTTATATAGATAGAGATTCTGAGACTTTGTTTAAGGA 544
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 299 AACTCCGAAAGGTTGGTTTATTACTTATATAGATAGAGATTCTGAGACTTTGTTTAAGGA 358
Query 545 GAGGTTGAAGAATAAGAGAGTTAAGAGTGATTTAGCTgaggaggagaagcaagagaggga 604
|||||||||||| ||||||||||||||||||||||||||||||||||| |||||||| ||
Sbjct 359 GAGGTTGAAGAACAAGAGAGTTAAGAGTGATTTAGCTGAGGAGGAGAAACAAGAGAGAGA 418
Query 605 gattcaga-gacagattgagagagctgctgagaAATtgaatggtggtggtggtgaaggtg 663
|||||||| | ||||||||||||||||||||||| | ||||| |||||||||||||||||
Sbjct 419 GATTCAGAAG-CAGATTGAGAGAGCTGCTGAGAAGTCGAATGCTGGTGGTGGTGAAGGTG 477
Query 664 aaaccagtggtaatgatgaagttgtggatgatggtgatgatgaGAGGAAGAAAGATGAAG 723
|||| |||||||| | ||||||| |||||| | || ||| || ||||||||||
Sbjct 478 AAACAAGTGGTAA--A-GAAGTTG---ATGATG---A-GA--AGA--AA-AAAGATGAAG 522
Query 724 ACTTGAGGTTGAAGAGTGGGGTCAAGGTTGGATTT 758
|||||||||||||||||||||| ||||||||||||
Sbjct 523 ACTTGAGGTTGAAGAGTGGGGTTAAGGTTGGATTT 557
> Ahg914671 jgi|Araly1|914671|Al_scaffold_0001_4925
Length=366
Score = 577 bits (312), Expect = 1e-163
Identities = 348/366 (95%), Gaps = 0/366 (0%)
Strand=Plus/Plus
Query 960 atgaaTCGGAAAGATTACTGGTTGTTCGAAGGGATCATTGTGAAGGTGATGAGTAAGGCT 1019
|||||||||||||| |||||||||||||| ||||| ||||||||||| ||||||||||||
Sbjct 1 ATGAATCGGAAAGACTACTGGTTGTTCGAGGGGATAATTGTGAAGGTAATGAGTAAGGCT 60
Query 1020 TTGGCAGAGAAAGGGTATTATAAGCAAAAAGGTGTTGTGAAGAAAGTGATTGATAATTAT 1079
||||||||||||||||||||||||||||| |||||||||||||||||||||||||| |||
Sbjct 61 TTGGCAGAGAAAGGGTATTATAAGCAAAAGGGTGTTGTGAAGAAAGTGATTGATAAGTAT 120
Query 1080 GTTGGGGAGATCAAAATGCTTGATTCTAAACATGTGTTGAGAGTTGATCAAAAGGAGCTA 1139
|| ||||||||| |||||||||||||||| |||||| |||||||||||||| ||||||
Sbjct 121 GTGGGGGAGATCGAAATGCTTGATTCTAAGCATGTGCTGAGAGTTGATCAAGTGGAGCTC 180
Query 1140 GAGACTGTGCTTCCACAAATTGGAGGGATGGTGAAGATAGTGAATGGGGCATATCGTGGT 1199
||||||||||||||||| || |||||||| ||||||||||||||||||||||||||||||
Sbjct 181 GAGACTGTGCTTCCACAGATAGGAGGGATAGTGAAGATAGTGAATGGGGCATATCGTGGT 240
Query 1200 TCGAATGCAAGGTTGTTGGGTGTGGATACAGAGAAGTTCTGTGCTAAAGTGCAGATCGAG 1259
|||||||||| |||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 241 TCGAATGCAAAGTTGTTGGGTGTGGATACAGAGAAGTTCTGTGCTAAAGTGCAAATCGAG 300
Query 1260 AAAGGTGTCTATGATGGAAGAGTCATCAAGTCCATTGAGTACGAGGACATATGCAAACTT 1319
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 301 AAAGGTGTCTATGATGGAAGAGTCATCAAGTCCATTGAGTACGAGGACATATGCAAACTT 360
Query 1320 GCTTAG 1325
||||||
Sbjct 361 GCTTAG 366
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 57935262645
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5