BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT1G57990.1
Length=1416
Score E
Sequences producing significant alignments: (Bits) Value
Ahg475452 jgi|Araly1|475452|fgenesh2_kg.2__582__AT1G57990.1 1940 0.0
Ahg475454 jgi|Araly1|475454|fgenesh2_kg.2__584__AT1G57943.2 1208 0.0
Ahg925250 jgi|Araly1|925250|scaffold_200811.1 979 0.0
Ahg925251 jgi|Araly1|925251|scaffold_200812.1 200 2e-50
> Ahg475452 jgi|Araly1|475452|fgenesh2_kg.2__582__AT1G57990.1
Length=1295
Score = 1940 bits (1050), Expect = 0.0
Identities = 1199/1271 (94%), Gaps = 10/1271 (1%)
Strand=Plus/Plus
Query 1 ATTTTCAAATCTTTAAAAGTTGAGACCAGAAGAAGAAGaaaaaaaaTCATGGAGATGACC 60
||||||||||||| ||||||||||||| |||||||| |||| |||||||||||||
Sbjct 1 ATTTTCAAATCTTCAAAAGTTGAGACC---AGAAGAAG---AAAACTCATGGAGATGACA 54
Query 61 GAAGCTTCCAAACAGACAACAGCAGAAGGATCAGCAAATCCAGAACCAGACCAAATCTTG 120
|||||||| |||||||||||| |||||| |||||||||||||||||||||||||||||||
Sbjct 55 GAAGCTTCAAAACAGACAACAACAGAAGAATCAGCAAATCCAGAACCAGACCAAATCTTG 114
Query 121 AGTCCGAGAAGATCGTTGGAGCTAAAACAAAAG-AAATGGTGGATCTCTGTTTCTTTATG 179
||||| |||||||| ||||||||||| | |||| ||||||||||||||||||||||||||
Sbjct 115 AGTCCAAGAAGATCATTGGAGCTAAATC-AAAGAAAATGGTGGATCTCTGTTTCTTTATG 173
Query 180 TATTTTCTTAGTCTTGCTCGGAGATTCTCTAGTCATGCTTCTCTTGAACTTCTTCTATGT 239
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 174 TCTTTTCTTAGTCTTGCTCGGAGATTCTCTAGTCATGCTTCTCTTGAACTTCTTCTATGA 233
Query 240 TCAAGACAATCGAGAAGATAGTGACCAAGATCTACAATACCGAGGAACATGGTTGCAAGC 299
|||| ||||||||||||| ||||| |||||||||||||||| ||||||||||||||||||
Sbjct 234 TCAAAACAATCGAGAAGACAGTGATCAAGATCTACAATACCAAGGAACATGGTTGCAAGC 293
Query 300 TCTGGTCCAAAACGCTGCGTTTCCACTACTCATTCCTCTGTTTTTCATTTTCCCTTCACC 359
||||||||||||||||||||||||||| ||||| ||||||||||||||||||||||||||
Sbjct 294 TCTGGTCCAAAACGCTGCGTTTCCACTCCTCATCCCTCTGTTTTTCATTTTCCCTTCACC 353
Query 360 AAAACAAAACCAAGAAACCACCAATACTCGTTTCCTCTCTTTTCGTCTCATCTTACTTTA 419
|||||||||||||||||||| ||| |||||||||||||||||||||||| ||||||||||
Sbjct 354 AAAACAAAACCAAGAAACCAACAACACTCGTTTCCTCTCTTTTCGTCTCCTCTTACTTTA 413
Query 420 CATCTCTCTTGGTGTTCTTGTTGCTGCTCACAGCAAATTGTTTGCACTTGGGAAATTATA 479
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 414 CATCTCTCTTGGTGTTCTTGTTGCTGCTCACAGCAAATTGTTTGCACTCGGGAAATTATA 473
Query 480 CGCAAACTTTGGCGTCTTCACGCTGATTTCCGCGACTCAGTTGATATTTACCGCTATTTT 539
||||||| ||||||||||||||| ||||||||||| |||||||||||||||||||||||
Sbjct 474 TGCAAACTATGGCGTCTTCACGCTCATTTCCGCGACCCAGTTGATATTTACCGCTATTTT 533
Query 540 CGCAGCCATTATTAACCGTTTTAAGTTCACCAGATGGATTATCTTATCGATAATCGGCAG 599
| ||||||| ||||||||||| ||||||||||||||||||||||||||||||||| ||
Sbjct 534 CACAGCCATCATTAACCGTTTCAAGTTCACCAGATGGATTATCTTATCGATAATCCTCAC 593
Query 600 CATTTTGATTTATGTTTTCGGTAGTCCTGAATTTGGAGGAGAGCCTGATGAAAACGAAGA 659
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 594 CATTTTGATTTATGTTTTTGGTAGTCCTGAATTTGGAGGAGAGCCTGATGAAAACGAAGA 653
Query 660 ATTCTACAGCATCCAAGCTTGGTTAACTTTCGCTGCTTCAGTTGCTTTCGCATTATCTCT 719
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 654 ATTCTACAACATCCAAGCTTGGTTAACTTTCGCTGCTTCAGTTGCTTTCGCATTATCTCT 713
Query 720 CTGTTTATTCCAACTTTGTTTCGAGAAAGTGTTGGTAAAGACAAAGAGATATGGTAACAA 779
||||||| ||||||| ||||||||||||||||||| |||||||||||||||||||| ||
Sbjct 714 CTGTTTAGTCCAACTCGGTTTCGAGAAAGTGTTGGTGAAGACAAAGAGATATGGTAATAA 773
Query 780 GAAAGTGTTTAGAATGGTCATAGAGATGCAAATTTGTGTCTCTTTTGTCGCAACGGTTGT 839
||||||||||||||||||| ||||||||||||| ||||||||||||||||| ||||||||
Sbjct 774 GAAAGTGTTTAGAATGGTCTTAGAGATGCAAATCTGTGTCTCTTTTGTCGCCACGGTTGT 833
Query 840 TTGTCTCGTGGGTTTGTTTGCGAGTGGCGAGAATAAGGAACTGCAAGGCGATAGCCACAG 899
||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 834 TTGTCTCGTGGGTATGTTTGCGAGTGGCGAGAATAAGGAACTGCAAGGCGATAGCCACAA 893
Query 900 GTTTAAGAAAGGAGAAACGTATTACGTTTTGAGTTTGATCGGGTTGGCATTGTCGTGGCA 959
|||||||||||||||| |||||||||| ||||||||||||||||||||||||| |||||
Sbjct 894 CTTTAAGAAAGGAGAAATGTATTACGTTATGAGTTTGATCGGGTTGGCATTGTCCTGGCA 953
Query 960 GGTTTGGGCGGTCGGGCTGATGGGTTTGGTGCTTTATGTTTCGGGTGTGTTTGGCGATGT 1019
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 954 GGTTTGGGCGGTCGGGCTGATAGGTTTGGTGCTTTATGTTTCGGGTGTGTTTGGCGATGT 1013
Query 1020 TGTTCATATGTGTACTTCACCACTTGTGGCTTTGTTTGTTGTGTTGGCATTTGATTTCAT 1079
||||||||||||||||| |||||||||||||| ||||||||||||||||||||||||||
Sbjct 1014 GGTTCATATGTGTACTTCCCCACTTGTGGCTTTATTTGTTGTGTTGGCATTTGATTTCAT 1073
Query 1080 GGATGATGAGTTTAGTTGGCCTAGAATTGGTACTTTGATAGCAACAG-TTGTGGCTTTAG 1138
|||||||||||||||||||||||||||||||||||||||||||||| || |||||||||
Sbjct 1074 GGATGATGAGTTTAGTTGGCCTAGAATTGGTACTTTGATAGCAACACCTT-TGGCTTTAG 1132
Query 1139 GATCTTACTTCTACACTCTGCATAAGAGAAACAAGAAGAAGATGGTGGAGCTTTACCAAA 1198
||||||||||||| || ||||||||||||||||| |||||||||||||| ||||||||||
Sbjct 1133 GATCTTACTTCTATACACTGCATAAGAGAAACAAAAAGAAGATGGTGGAACTTTACCAAA 1192
Query 1199 CAGAGAACAATATTGACGTTTAGTCTCTCATGTCAAATGTTTGTTTCTGTTTCAAAATAT 1258
|||||||||||||||| ||||||| ||||||| ||||||| ||||| |||||||||||||
Sbjct 1193 CAGAGAACAATATTGAAGTTTAGTATCTCATGACAAATGTATGTTTGTGTTTCAAAATAT 1252
Query 1259 GTTGTTGTTCT 1269
||| |||||||
Sbjct 1253 GTTCTTGTTCT 1263
> Ahg475454 jgi|Araly1|475454|fgenesh2_kg.2__584__AT1G57943.2
Length=1180
Score = 1208 bits (654), Expect = 0.0
Identities = 1027/1201 (86%), Gaps = 49/1201 (4%)
Strand=Plus/Plus
Query 49 ATGGAGATGACCGAAGCTTCCAAACAGACAACAGCAGA-AGGA-TCAGCAAATCCAGAAC 106
||||||||| || ||||||||||||||||| || ||| | | |||| ||||||||||
Sbjct 1 ATGGAGATGTCCAAAGCTTCCAAACAGACAGCA--AGACA-TATTCAG--AATCCAGAAC 55
Query 107 CAG-ACCAAATCTTGAGTCCGAGAAGATCGTTGGAGCTAAAACAAAAG-AAATGGTGGAT 164
| | |||||| ||||||||| |||||||| ||| ||||||||| |||| |||||||||||
Sbjct 56 C-GTACCAAAGCTTGAGTCCAAGAAGATCATTGAAGCTAAAAC-AAAGAAAATGGTGGAT 113
Query 165 CTCTGTTTCTTTATGTATTTTCTTAGTCTTGCTCGGAGATTCTCTAGTCATGCTTCTCTT 224
|||||||||||||||||||||||||||||||||||| |||||||| ||||||||||||||
Sbjct 114 CTCTGTTTCTTTATGTATTTTCTTAGTCTTGCTCGGTGATTCTCTCGTCATGCTTCTCTT 173
Query 225 GAACTTCTTCTATGTTC-AA--GA-C-A-A---TCGA-G-AAGATAGTGACCAAGATCTA 273
|||||||||||| |||| || || | | | || | | || || ||||||||||||
Sbjct 174 GAACTTCTTCTACGTTCAAATGGAGCAAGACTTTC-ATGAAATTTA-TGACCAAGATCTG 231
Query 274 CAATACCGAGGAACATGGTTGCAAGCTCTGGTCCAAAACGCTGCGTTTCC-ACTACTCAT 332
|||||| |||||||||| |||||||||| ||||||||||||||||||| | | |||||
Sbjct 232 CAATACAAAGGAACATGGACGCAAGCTCTGATCCAAAACGCTGCGTTTCCTA-TCCTCAT 290
Query 333 TCCTCTGTTTTTCATTTTCCCTTCACCAAAACAAAACCAAGAAACCACCAATACTCGTTT 392
|||||| || |||||||||||| |||||||||||||| ||||||| ||||||| | ||
Sbjct 291 TCCTCTCTTCTTCATTTTCCCTAAACCAAAACAAAACCTTGAAACCAACAATACTAGATT 350
Query 393 CCTCTCTTTTCGTCTCATCTTACTTTACATCTCTCTTGGTGTTCTTGTTGCTGCTCACAG 452
|||||| || |||||| |||| |||||| | |||||||||||||||||||||||||||||
Sbjct 351 CCTCTCCTTGCGTCTCTTCTTCCTTTACTTATCTCTTGGTGTTCTTGTTGCTGCTCACAG 410
Query 453 CAAATTGTTTGCACTTGGGAAATTA-TACGCAAACTTTGGCGTCTTCACGCTGATTTCCG 511
||||||||||||||| || |||||| | | |||||| |||| |||||| | |||||||||
Sbjct 411 CAAATTGTTTGCACTCGGCAAATTAGT-CTCAAACTATGGCATCTTCATGTTGATTTCCG 469
Query 512 CGACTCAGTTGATATTTACCGCTATTTTCGCAGCCATTATTAACCGTTTTAAGTTCACCA 571
| || || |||||||||||||||||||| |||| || |||||||| || ||||| || |
Sbjct 470 CAACCCAATTGATATTTACCGCTATTTTAACAGCTATCATTAACCGCTTCAAGTTTACTA 529
Query 572 GATGGATTATCTTATCGATAAT-CGGCAGC-ATTTTGATTTATGTTTTCGGTAGT-CCTG 628
||||||| ||| |||||||| | | || | ||| ||||||| ||| || ||| | ||||
Sbjct 530 GATGGATCATCATATCGATATTACT-CA-CTATTGTGATTTACGTTCTCAGTA-TACCTG 586
Query 629 AATTTGGAGGAGAGCCTGATGAAA-ACGAAGAAT-TC--TACAGCATCCAAGCTTGGTTA 684
| ||||||||| ||||| |||| | ||||||| | |||| ||||||||||||||||
Sbjct 587 ACTTTGGAGGACAGCCTCATGAGGGA-GAAGAATATGGATACAACATCCAAGCTTGGTTA 645
Query 685 ACTTTCGCTGCTTC-AGTTGCTTTCGCATTATCTCTCTGTTTATTCCAACTTTGTTTCGA 743
||||| ||||| | | |||||||| ||||||||||||| ||| ||||||| | |||||
Sbjct 646 GCTTTCTCTGCTACGA-TTGCTTTCTCATTATCTCTCTGCTTAATCCAACTCGGGTTCGA 704
Query 744 GAAAGTGTTGGTAAAGACAAAGAGATATGGTAACAAGAAAGTGTTTAGAATGGTCATAGA 803
|||| | |||| |||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct 705 GAAACTCCTGGTGAAGACAAAGAGATATGGTAACAAGAAAGTGTTTAGAATGGTCTTAGA 764
Query 804 GATGCAAATTTGTGTCTCTTTTGTCGCAACGGTTGTTTGTCTCGTGGGTTTGTTTGCGAG 863
||||||||| ||||||||||||||||| || || ||||||| |||||||||||||| ||
Sbjct 765 GATGCAAATATGTGTCTCTTTTGTCGCCTCGATTATTTGTCTTGTGGGTTTGTTTGCAAG 824
Query 864 TGGCGAGAATAAGGAACTGCAAGGCGATAGCCACAGGTTTAAGAAAGGAGAAACGTATTA 923
||| ||| ||||||||||| |||| |||||| | |||||||||||||||||| |||||||
Sbjct 825 TGGTGAGTATAAGGAACTGAAAGGTGATAGCAAGAGGTTTAAGAAAGGAGAAGCGTATTA 884
Query 924 CGTTTTGAGTTTGATCGGGTTGGCATTGTCGTGGCAGGTTTGGGCGGTCGGGCTGATGGG 983
|||| |||||||| |||||||||| ||||||||||||||||||||||||||||| || ||
Sbjct 885 CGTTCTGAGTTTGGTCGGGTTGGCTTTGTCGTGGCAGGTTTGGGCGGTCGGGCTTATAGG 944
Query 984 TTTGGTGCTTTATGTTTCGGGTGTGTTTGGCGATGTTGTTCATATGTGTACTTCACCACT 1043
|||||||||||||||||||||| | ||||| ||| ||||||||||||| ||||||||||
Sbjct 945 TTTGGTGCTTTATGTTTCGGGTCTTTTTGGTGATATTGTTCATATGTGCGCTTCACCACT 1004
Query 1044 TGTGGCTTTGTTTGTTGTGTTGGCATTTGATTTCATGGATGATGAGTTTAGTTGGCCTAG 1103
|||||||||||||||||| |||||||||||||| |||||||||| |||||||||| ||||
Sbjct 1005 TGTGGCTTTGTTTGTTGTCTTGGCATTTGATTTTATGGATGATGTGTTTAGTTGGACTAG 1064
Query 1104 AATTGGTACTTTGAT-AGCAACAG-TTGTGGCTTTAGGATCTTACTTCTACACTCTGCAT 1161
||||||| |||| || || |||| || ||||||| |||||||||||||||||||| ||
Sbjct 1065 AATTGGTGCTTT-ATTAGGAACAACTT-TGGCTTTGGGATCTTACTTCTACACTCTACAC 1122
Query 1162 AAGAGAAACAAGAAGAAGATGGTG-GAGCTTTACCAAACAGAGAACAATATTGACGTTTA 1220
||||||||||||||||||||| | || ||| | ||| | ||||||| |||| |||||
Sbjct 1123 AAGAGAAACAAGAAGAAGATG-TCCGAACTTAATTAAA--GTGAACAATGTTGAAGTTTA 1179
Query 1221 G 1221
|
Sbjct 1180 G 1180
> Ahg925250 jgi|Araly1|925250|scaffold_200811.1
Length=918
Score = 979 bits (530), Expect = 0.0
Identities = 695/774 (90%), Gaps = 14/774 (2%)
Strand=Plus/Plus
Query 475 TTATACGCAAACTTTGGCGTCTTCACGCTGATTTCCGCGACTCAGTTGATATTTACCGCT 534
|||||| |||||| ||||||||||||||||||||||||||| |||||||| ||||||| |
Sbjct 147 TTATACTCAAACTATGGCGTCTTCACGCTGATTTCCGCGACCCAGTTGATCTTTACCG-T 205
Query 535 -ATTTTCGCAGCCATTATTAACCGTTTTAAGTTCACCAGATGGATTATCTTATCGATAAT 593
||||| |||||||||| |||||||| ||||| ||||||||||||||| ||||||||||
Sbjct 206 GGTTTTCTCAGCCATTATCAACCGTTTCAAGTTTACCAGATGGATTATCATATCGATAAT 265
Query 594 CGGCAGCATTTTGATTTATGTTTTCGGTAGTCCTGAATTTGGAGGAGAGCCTGATGAAAA 653
| || ||||||||| ||||||||||||||||||||||| | ||||||||||||||||||
Sbjct 266 CCTCACCATTTTGATATATGTTTTCGGTAGTCCTGAATTCGCAGGAGAGCCTGATGAAAA 325
Query 654 CGAAGAATTCTACAG-CATCCAAGCTTGGTTAACTTTCGCTGCTTCAGTTGCTTTCGCAT 712
||||||||||||| | ||||||||||||||||||||| |||||||||||||| ||| | |
Sbjct 326 CGAAGAATTCTAC-GACATCCAAGCTTGGTTAACTTTTGCTGCTTCAGTTGCCTTCCCTT 384
Query 713 TATCTCTCTGTTTATTCCAACTTTGTTTCGAGAAAGTGTTGGTAAAGACAAAGAGATATG 772
|||||| ||||||| ||||||| | ||||||||| | ||||| ||||||||||||||||
Sbjct 385 TATCTCCCTGTTTAAGCCAACTTGGGTTCGAGAAACTATTGGTGAAGACAAAGAGATATG 444
Query 773 GTAACAAGAAAGTGTTTAGAATGGTCATAGAGATGCAAATTTGTGTCTCTTTTGTCGCAA 832
|||||||||||||||||||||||||| | ||| | |||||||||||||||||||| ||
Sbjct 445 GTAACAAGAAAGTGTTTAGAATGGTCTTGGAGCTACAAATTTGTGTCTCTTTTGTTGCCT 504
Query 833 CGGTTGTTTGTCTCGTGGGTTTGTTTGCGAGTGGCGAGAATAAGGAACTGCAAGGCGATA 892
||||||||||||| |||||||||||||||||||||||| || |||||||| |||||||||
Sbjct 505 CGGTTGTTTGTCTTGTGGGTTTGTTTGCGAGTGGCGAGTATGAGGAACTGAAAGGCGATA 564
Query 893 GCCACAGGTTTAAGAAAGGAGAAACGTATTACGTT-TTGAGTTT-GATCGGGTTGGCATT 950
|| | |||||||||||||| ||||| ||||||||| || ||||| | || ||||||| ||
Sbjct 565 GCAAGAGGTTTAAGAAAGGGGAAACATATTACGTTCTT-AGTTTGGTTC-GGTTGGCTTT 622
Query 951 GTCGTGGCAGGTTTGGGCGGTCGGGCTGATGGGTTTGGTGCTTTATGTTTCGGGTGTGTT 1010
|||||||||||||||| ||||||||||||| ||||||||||| |||||||| ||||||||
Sbjct 623 GTCGTGGCAGGTTTGGTCGGTCGGGCTGATAGGTTTGGTGCTCTATGTTTCCGGTGTGTT 682
Query 1011 TGGCGATGTTGTTCATATGTGTACTTCACCACTTGTGGCTTTGTTTGTTGTGTTGGCATT 1070
||| ||||||||||||||||||||||||||||||||||||||||||||||| ||||| ||
Sbjct 683 TGGTGATGTTGTTCATATGTGTACTTCACCACTTGTGGCTTTGTTTGTTGTCTTGGCGTT 742
Query 1071 TGATTTCATGGATGATGAGTTTAGTTGGCCTAGAATTGGTACTTTGATAGCAACAGTTGT 1130
|||||| ||||||||||||||||||||||| || |||||||||||||||||||||||| |
Sbjct 743 TGATTTTATGGATGATGAGTTTAGTTGGCCAAGGATTGGTACTTTGATAGCAACAGTTTT 802
Query 1131 GGCTTTAGGATCTTACTTCTACACTCTGCATAAGAGAAACAAGAAGAAGATGGTGGAGCT 1190
||||||||||||||||||||| || ||||| |||||||||||||||||||||||||| ||
Sbjct 803 GGCTTTAGGATCTTACTTCTATACGCTGCACAAGAGAAACAAGAAGAAGATGGTGGAACT 862
Query 1191 TTACCAAACAGAGAAC-A-A-TATTGACGTTTAGTCTCTCAT---GTCAAATGT 1238
| |||||| ||||||| | | |||||| ||||||||| | || |||||||||
Sbjct 863 TAACCAAAGAGAGAACTATAATATTGAAGTTTAGTCTATTATTATGTCAAATGT 916
> Ahg925251 jgi|Araly1|925251|scaffold_200812.1
Length=236
Score = 200 bits (108), Expect = 2e-50
Identities = 188/223 (84%), Gaps = 19/223 (9%)
Strand=Plus/Plus
Query 42 aaaaaTCATGGAGATGA-CCGAAGCTTCCAAACAGACAAC-A-GCA-GAAG-----GATC 92
|||| | |||||||||| || ||||||| ||||||||||| | || |||| || |
Sbjct 15 AAAACTTATGGAGATGAGCC-AAGCTTCTAAACAGACAACAATACATGAAGAATCAGAAC 73
Query 93 A-G--CA-AATCCAGAACCAGACCAAATCTTGAGTCCGAGAAGATCGTTGGAGCTAAAAC 148
| | || ||||||||||||||||||||||| ||||| ||||||||||||||||| || |
Sbjct 74 ATGTTCAGAATCCAGAACCAGACCAAATCTTCAGTCCAAGAAGATCGTTGGAGCTCAATC 133
Query 149 AAAAGAAATGGTGGATCTCTGTTTCTTTATGTATTTTCTTAGTCTTGCTCGGAGATTCTC 208
||||||||||||||||||||||| | ||||| ||||||||||| |||| ||||||||||
Sbjct 134 AAAAGAAATGGTGGATCTCTGTTGCGCTATGTCTTTTCTTAGTCCTGCTTGGAGATTCTC 193
Query 209 TAGTCATGC--T--T-CTCTTGAACTTCTTCTATGTTCAAGAC 246
| ||||||| | | |||||||||||||||||||||||||||
Sbjct 194 TCGTCATGCGATGCTTCTCTTGAACTTCTTCTATGTTCAAGAC 236
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 52945440550
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5