BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT1G66700.1
Length=1299
Score E
Sequences producing significant alignments: (Bits) Value
Ahg475766 jgi|Araly1|475766|fgenesh2_kg.2__896__AT1G66700.1 1886 0.0
Ahg338902 jgi|Araly1|338902|fgenesh1_pg.C_scaffold_2001001 841 0.0
Ahg925862 jgi|Araly1|925862|scaffold_201423.1 562 2e-159
Ahg908199 jgi|Araly1|908199|Al_scaffold_0002_1280 388 4e-107
> Ahg475766 jgi|Araly1|475766|fgenesh2_kg.2__896__AT1G66700.1
Length=1277
Score = 1886 bits (1021), Expect = 0.0
Identities = 1197/1282 (93%), Gaps = 11/1282 (1%)
Strand=Plus/Plus
Query 1 ACTACTCTCATCATAAACCAAAGTATCAAACAATAAAAGATTTTTGAGAAAAA-AGAGAG 59
|||||||||||||||||||||||||| |||||| |||||| ||||||||||| ||||||
Sbjct 1 ACTACTCTCATCATAAACCAAAGTATTAAACAAGAAAAGAAGTTTGAGAAAAAGAGAGAG 60
Query 60 AGAGATATCATGACTACTACTCCAGATTGGATCATGATTGGAGGAGACGGTCCTGAGAGT 119
||||| ||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 61 AGAGAGATCATGACTACTACTCCAGATTGGATCATGATTGGAGGAGATGGTCCTGAGAGT 120
Query 120 TATAACCAACAATCCTCGTATCAGAGAGCATTGTTGGAAGC-GACAAAGGACAAGATGAC 178
|| |||||||| ||||||||||||||||| ||||||||||| | ||||||||||||||||
Sbjct 121 TACAACCAACAGTCCTCGTATCAGAGAGCGTTGTTGGAAGCTG-CAAAGGACAAGATGAC 179
Query 179 CAAGGCGATCTCAGCCAATCTCGACCTAGACTTGATTTCGAATCGCTTCATTGTAGCGGA 238
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 180 CGAGGCGATCTCAGCCAATCTCGACCTAGACTTGATTTCGAATCGCTTCATTGTAGCGGA 239
Query 239 TTTCGGTTGTGCAAGTGGACCTAACACTTTTGTGGCAGTTCAAAACATAATAGATGCGGT 298
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 240 TTTCGGTTGTGCGAGTGGACCTAACACTTTTGTGGCAGTTCAAAACATAATAGATGCGGT 299
Query 299 AGAAGAAAAGTACCGTAGAGAAACCGGACAAAACCCGGCA-GATAACATCGAGTTCCAAG 357
|||||||||||||||||||||||||||||||||||| | | |||||||||||||||||||
Sbjct 300 AGAAGAAAAGTACCGTAGAGAAACCGGACAAAACCCCG-ATGATAACATCGAGTTCCAAG 358
Query 358 TCCTCTTCAATGATTTCAGCCTCAATGATTTCAACACTCTCTTCCAGACACTTCCACCCG 417
|||| ||||| || |||||||| || ||||||||||||||||||||||||||||| || |
Sbjct 359 TCCTATTCAACGACTTCAGCCTGAACGATTTCAACACTCTCTTCCAGACACTTCCTCCAG 418
Query 418 GAAGAAGATACTTCAGCGCTGGAGTTCCTGGTTCCTTCTTCGAACGTGTTCTTCCTAAGG 477
| |||| |||||| |||||||| ||||||||||||||||| |||||||||||||| ||||
Sbjct 419 GGAGAAAATACTTTAGCGCTGGGGTTCCTGGTTCCTTCTTTGAACGTGTTCTTCCGAAGG 478
Query 478 AGAGTTTCCACATCGGAGTCATGAGTTACGCGTTCCATTTCACCTCCAAAATCCCCAAAG 537
|||||||||||||||||||||||||||| || ||||||||||||||||||||||||||||
Sbjct 479 AGAGTTTCCACATCGGAGTCATGAGTTATGCATTCCATTTCACCTCCAAAATCCCCAAAG 538
Query 538 GGATTATGGACCGCGACTCTCCCTTGTGGAACAAAGACATGCAGTGCACGGGGTTCAACC 597
| || |||||||||||||||||||||||||||||||||||||| ||||| ||||||||||
Sbjct 539 GAATCATGGACCGCGACTCTCCCTTGTGGAACAAAGACATGCAATGCACCGGGTTCAACC 598
Query 598 CCGCTGTCAAGAAAGCGTATCTTGACCAGTACTCTATCGACACCAAAATTCTTTTAGATG 657
| ||||||||||||||||| ||||| ||||||||||| ||||||||||||||||| ||||
Sbjct 599 CAGCTGTCAAGAAAGCGTACCTTGATCAGTACTCTATTGACACCAAAATTCTTTTGGATG 658
Query 658 CTAGAGCTGAAGAGCTCGTGCCCGGGGGGTTGATGTTGCTTTTAGGATCGTGTCTAAGAG 717
||||||||||||||||||| |||||||| |||||||||||||||||||| ||||||||||
Sbjct 659 CTAGAGCTGAAGAGCTCGTACCCGGGGGATTGATGTTGCTTTTAGGATCTTGTCTAAGAG 718
Query 718 ACGGAGTTAAGATGTCCGAGACCCCTAAAGGAACTGTAATGGATTTTATTGGAGAATCTC 777
||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| |
Sbjct 719 ACGGAGTTAAGATGTCCGAGACCCCTAAAGGAACTGTCATGGATTTTATTGGAGAATCAC 778
Query 778 TTAGCGATCTTGCTAAACAGGGTGTCACCGAGCAAGAGAAGGTGGACACTTTCAGAACCT 837
||| ||||||||| |||||||||||||||||||||| ||||||||||| |||| |||||
Sbjct 779 TTATTGATCTTGCTCAACAGGGTGTCACCGAGCAAGAAAAGGTGGACACATTCAAAACCT 838
Query 838 CAATTTACTTTGCAGAACAAGGTGAGATAAGGCAAATCATTGAGGAGAATGGGAAGTTCA 897
|||||||||||||||| ||||| |||||||||||||||||||| ||||||||||||||||
Sbjct 839 CAATTTACTTTGCAGAGCAAGGCGAGATAAGGCAAATCATTGAAGAGAATGGGAAGTTCA 898
Query 898 CAATCGAGGCTTTT-GAAGATATCATTCACGCTAAAAACGAGTTTCCTTTTGACCCCAAG 956
||||||| |||||| || |||||||||||| |||| || |||||||| | |||||||||
Sbjct 899 CAATCGA-GCTTTTCGAGGATATCATTCACTCTAAGAATGAGTTTCCGCTAGACCCCAAG 957
Query 957 ACATTGGCCATCTCTTTCAAGGCCTTCTATGGTGCTTTCATTTCCGCACATTTTGGAGTC 1016
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 958 ACATTGGCCATCTCTTTCAAGGCCTTCTATGGTGCTTTCATTTCCGCACATTTTGGAGTC 1017
Query 1017 GAAGTCATGAGGAAAGCCTTTGAGCTTGTTGAGGTCAAGGCACGCGAACAGATTTCTCGC 1076
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 1018 GAAGTCATGAGGAAAGCCTTTGAGCTTGTCGAGGTCAAGGCACGCGAACAGATTTCTCGC 1077
Query 1077 CTCCATAACT-CCAAACCCGGAATGCAGTACCTCATCGTGCTTCGCAAGAACTAAACCGT 1135
|| ||||| | ||||| ||||||||||||||||||||||||||||||||||||| ||||
Sbjct 1078 CTACATAA-TGCCAAAGACGGAATGCAGTACCTCATCGTGCTTCGCAAGAACTAATCCGT 1136
Query 1136 GATCCAAATATATTGTTATATGTGATTTCCTCCATTGTTGcttctcttctttcgtctatc 1195
|||| |||||||||| || ||||||||||||||||||||||||||||||||||||| |||
Sbjct 1137 GATCGAAATATATTGATA-ATGTGATTTCCTCCATTGTTGCTTCTCTTCTTTCGTCGATC 1195
Query 1196 ttatggtgctttgtcgtctctt-cttcttgttgttttgtcttAACGTCTTTGTATTCTCC 1254
| ||||||| ||||||||| || ||||||| |||||||||||| ||||||||| |||||
Sbjct 1196 TCATGGTGCATTGTCGTCTTTTTCTTCTTGCTGTTTTGTCTTAGTGTCTTTGTACTCTCC 1255
Query 1255 CCTATCCTACAAATGAATAAAA 1276
|||||||| ||||| |||||||
Sbjct 1256 CCTATCCTTCAAATTAATAAAA 1277
> Ahg338902 jgi|Araly1|338902|fgenesh1_pg.C_scaffold_2001001
Length=1059
Score = 841 bits (455), Expect = 0.0
Identities = 879/1080 (81%), Gaps = 43/1080 (4%)
Strand=Plus/Plus
Query 69 ATGACTACTACTCCAGATTGGATCATGATTGGAGGAGACGGTCCTGAGAGTTATAACCAA 128
||| || |||||||||| ||| ||||| |||||||||| |||||||||||||| || ||
Sbjct 1 ATGTCTCCTACTCCAGAATGGGTCATGGTTGGAGGAGAAGGTCCTGAGAGTTACAAGCAG 60
Query 129 CAATCCTCGTATCAGAGAGCA-TTGTTGGAAGCGA-CAAAGGA-CAAGATGACCAAGGCG 185
|| || ||||||||||||| | |||||| |||| | ||||||| || || | | || |
Sbjct 61 CATTCTTCGTATCAGAGAG-ATTTGTTGAAAGC-AGCAAAGGATGAA-ATAAACGCGGTG 117
Query 186 ATCTCAGCCAATCTCGA-CCTAGACTTGATTTCGAATCGCTTCATTGTAGCGGATTTCGG 244
||| |||| || ||| | ||| || |||||||||| ||| |||| ||| |||||||||||
Sbjct 118 ATCGCAGCGAACCTC-AGCCTCGATTTGATTTCGAGTCGGTTCAGTGTTGCGGATTTCGG 176
Query 245 TTGTGCAAGTGGACCTAACACTTTTGTGGCAGTTCAAAACATAATAGATGCGGTAGAAGA 304
|||||| |||||||||||||||||| ||||||| ||||||||||||||||| || |||||
Sbjct 177 TTGTGCGAGTGGACCTAACACTTTTATGGCAGTCCAAAACATAATAGATGCCGTGGAAGA 236
Query 305 AAAGTACCGTAGAGAAACCGGACAAAACCCGGCAGATAACATCGAGTTCCAAGTCCTCTT 364
||||| | |||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 237 GAAGTATCTTAGAGAAACCGGACAAAACCCGGCGGATAACATCGAGTTCCAAGTCCTCTT 296
Query 365 CAATGATTTCAGCC-TCAATGATTTCAACACTCTCTTCCAGACACTTCCACCCG-GAAGA 422
||| || || ||| | || ||||||||||||||||||||| |||||| | | | ||
Sbjct 297 CAACGACTTAAGCAAT-AACGATTTCAACACTCTCTTCCAGGGACTTCCTTC-GAGCAGG 354
Query 423 AGATACT-TCAGCGCTGGAGTTCCTGGTTCCTTCTTCGAACGTGTTCTTCCTAAGGAGAG 481
||||||| | || ||||| || || |||||||||||||| ||||||||||||||| ||||
Sbjct 355 AGATACTAT-AGTGCTGGCGTCCCCGGTTCCTTCTTCGACCGTGTTCTTCCTAAGCAGAG 413
Query 482 TTTCCACATCGGAGTCATGAGTTACGCGTTCCATTTCACCTCCAAAATCCCCAAAGGGAT 541
| ||||||| ||||| |||| |||||| || || ||||||||||||||||||||||||||
Sbjct 414 TATCCACATAGGAGTTATGAATTACGCTTTTCAGTTCACCTCCAAAATCCCCAAAGGGAT 473
Query 542 -TATGGACCGCGACTCTCCCTTGTGGAACAAAGACATGCAGTGCACGGGGTTCAACCCCG 600
| | ||||| |||||||| ||||||||| |||||| || ||||| || |||||| |
Sbjct 474 CTCCG-ACCGCAACTCTCCCATGTGGAACAGAGACATACATTGCACTGGCTTCAACAACA 532
Query 601 CTGTCAAGAAAGCGTATCTTGACCAGTACTCTATCGACACCAA-AATTCTTTTAGATGCT 659
||||||||||||||| |||| ||| |||| ||||| |||| ||| | || ||||||
Sbjct 533 AGGTCAAGAAAGCGTATTTTGATCAGGACTCGCTCGACTCCAAGAATA-TATTGGATGCT 591
Query 660 AGAGCTGAAGAGCTCGTGCCCGGGGGGTTGATGTTGCTTTTAGGATCGTGTCTAAGAGAC 719
|||||||||||||| | |||||||| || | ||||||||||||||||||||||||||
Sbjct 592 CGAGCTGAAGAGCTCTTACCCGGGGGACTGTTATTGCTTTTAGGATCGTGTCTAAGAGAA 651
Query 720 GG-AGTTAAGATGTCCGAGACCCCTAAAGGAACT-GTAATGGATTTTATTGGAGAATCTC 777
|| | | |||||||| || ||| || ||||| | || ||||||| || |||| |||
Sbjct 652 GGTA-TCAAGATGTCGGAAACCTATAGAGGAA-TAGTGCTGGATTTAATCGGAGCTTCT- 708
Query 778 TTAGC-GATCTTGCTAA-ACAGGGTGTCACCGAGCAAGAGAAGGTGGACACTTTCAGA-A 834
||| ||||||||| | | ||||||||| ||||||||| ||||||||| |||||| | |
Sbjct 709 TTAAATGATCTTGCTCAGA-AGGGTGTCATCGAGCAAGACAAGGTGGACTCTTTCA-ATA 766
Query 835 CCTCAATTTACTTTGCAGAACAAGGTGAGATAAGGCAAATCATTGAGGAGAATGGGAAGT 894
| || | ||| ||| || ||| ||| | |||||||||||||| ||||| |||||||
Sbjct 767 TCACACTCTACACTGCCGAGGAAGACGAGTTGAGGCAAATCATTGAAGAGAACGGGAAGT 826
Query 895 TCACAATCGAGGCTTTTGAAGATATCATTCACGCTAAAAACGAGTT-TC-CTTTTGACCC 952
||||||| || ||||| || ||||||||||| | | ||| || | || ||| |||||
Sbjct 827 TCACAATTGAAGCTTTCGAGGATATCATTCA-G-TCAAA-CGGGGAGTCGCTT--GACCC 881
Query 953 CAAGA-CATTGGCCATCTCTTTCAAGGCCTTC-TATGGTGCTTTCATTTCCGCACATTTT 1010
||||| | ||||| |||| || ||| | | | | ||| | | |||| ||| ||||||||
Sbjct 882 CAAGATC-TTGGCTGTCTCCTTGAAGTC-TGCCTTTGGAGGTATCATATCCTCACATTTT 939
Query 1011 GGAGTCGAAGTCATGAGGAAAGCCTTTGAGCTTGTTGAGGTCAAGGCACGCGAACAGATT 1070
|||| ||||| ||||||||||||||||||||| || |||| |||||||| | || | |||
Sbjct 940 GGAGCCGAAGCCATGAGGAAAGCCTTTGAGCTCGTCGAGGCCAAGGCACACCAAGA-ATT 998
Query 1071 T-CTCGCCTCCATAACT-CCAAACCCGGAATGCAGTACCTCATCGTGCTTCGCAAGAACT 1128
| |||||||| | || | ||||||||| |||||| ||||| ||||| |||||||||||||
Sbjct 999 TTCTCGCCTCAAGAA-TGCCAAACCCGCAATGCAATACCTTATCGTACTTCGCAAGAACT 1057
> Ahg925862 jgi|Araly1|925862|scaffold_201423.1
Length=758
Score = 562 bits (304), Expect = 2e-159
Identities = 513/613 (84%), Gaps = 17/613 (3%)
Strand=Plus/Plus
Query 46 GAGAAAAAAGAGAGAGAGATATCATGACTACTACTCCAGATTGGATCATGATTGGAGGAG 105
|||| |||||| ||||||| || ||| || |||||||||| ||| ||||| |||||||||
Sbjct 1 GAGAGAAAAGAAAGAGAGA-ATAATGTCTCCTACTCCAGAATGGGTCATGGTTGGAGGAG 59
Query 106 ACGGTCCTGAGAGTTATAACCAACAATCCTCGTATCAGAGAGCA-TTGTTGGAAGCGA-C 163
| |||||||||||||| || || || || |||||||| |||| | ||| || |||| | |
Sbjct 60 AAGGTCCTGAGAGTTACAAGCAGCATTCTTCGTATCAAAGAG-ATTTGCTGAAAGC-AGC 117
Query 164 AAAGGACAAGATGACCAAGGCGATCTCAGCCAATCTCGA-CCTAGACTTGATTTCGAATC 222
|||||| || || | | || ||||||||| || ||| | ||| || |||||||||||||
Sbjct 118 AAAGGATAAAATAAACGCGGTGATCTCAGCAAACCTC-AGCCTCGATTTGATTTCGAATC 176
Query 223 GCTTCATTGTAGCGGATTTCGGTTGTGCAAGTGGACCTAACACTTTTGTGGCAGTTCAAA 282
| || | ||| ||||||||||||||||| |||||||||||||||||||||||||| ||||
Sbjct 177 GGTTTAGTGTTGCGGATTTCGGTTGTGCGAGTGGACCTAACACTTTTGTGGCAGTCCAAA 236
Query 283 ACATAATAGATGCGGTAGAAGAAAAGTACCGTAGAGAAACCGGACAAAACCCGGCAGATA 342
||||||||||||| || ||||| ||||| | |||||||||||||||||||||| ||||||
Sbjct 237 ACATAATAGATGCCGTGGAAGAGAAGTATCTTAGAGAAACCGGACAAAACCCGTCAGATA 296
Query 343 ACATCGAGTTCCAAGTCCTCTTCAATGATTTCAGCC-TCAATGATTTCAACACTCTCTTC 401
||||||||||||||||||||||||| || || ||| | || ||||||||||||||||||
Sbjct 297 ACATCGAGTTCCAAGTCCTCTTCAACGACTTAAGCAAT-AACGATTTCAACACTCTCTTC 355
Query 402 C-AGACACTTCCACCCGGA-AGAAGATACTTCAGCGCTGGAGTTCCTGGTTCCTTCTTCG 459
| || ||||||| | ||| || ||||||| ||| ||||| ||||| | |||||||||||
Sbjct 356 CGAG-CACTTCCTTC-GGACAGGAGATACTACAGTGCTGGCGTTCCCGATTCCTTCTTCG 413
Query 460 AACGTGTTCTTCCTAAGGAGAGTTTCCACATCGGAGTCATGAGTTACGCGTTCCATTTCA 519
| |||||||||||||| ||||| ||||||| |||||||||| |||||| || || ||||
Sbjct 414 ACCGTGTTCTTCCTAAACAGAGTATCCACATAGGAGTCATGAATTACGCTTTTCAGTTCA 473
Query 520 CCTCCAAAATCCCCAAAGGGAT-TATGGACCGCGACTCTCCCTTGTGGAACAAAGACATG 578
|||||||||||||||||||||| | | ||||| |||||||| ||||||||| ||||||
Sbjct 474 CCTCCAAAATCCCCAAAGGGATCTCCG-ACCGCAACTCTCCCCTGTGGAACAGAGACATA 532
Query 579 CAGTGCACGGGGTTCAACCCCGCTGTCAAGAAAGCGTATCTTGACCAGTACTCTATCGAC 638
|| ||||| || |||||| | |||||||| || ||| |||| |||||||| |||||
Sbjct 533 CATTGCACTGGCTTCAACAACAAGGTCAAGAAGGCATATTTTGATCAGTACTCGCTCGAC 592
Query 639 ACCAA-AAT-TCT 649
|||| ||| |||
Sbjct 593 TCCAAGAATATCT 605
> Ahg908199 jgi|Araly1|908199|Al_scaffold_0002_1280
Length=534
Score = 388 bits (210), Expect = 4e-107
Identities = 301/345 (87%), Gaps = 6/345 (2%)
Strand=Plus/Plus
Query 98 TGGAGGAGACGGTCCTGAGAGTTATAACCAACAATCCTCGTATCAGAGAGCATTGTTGGA 157
||| ||||| |||||||||||||||||||||||||| |||||||||||||||||| | ||
Sbjct 15 TGGTGGAGAAGGTCCTGAGAGTTATAACCAACAATCTTCGTATCAGAGAGCATTGCTAGA 74
Query 158 AGCGACAAAGGACAAGATGACCAAGGCGATCTCAGCCAATCTCGACCTAGACTTGATTTC 217
|| | ||||||| ||||||||| ||||||||||||| || ||| |||| || ||||||||
Sbjct 75 AGTGGCAAAGGAAAAGATGACCGAGGCGATCTCAGCGAACCTCAACCTCGATTTGATTTC 134
Query 218 GAATC-GCTTCATTGTAGCGGATTTCGGTTGTGCAAGTGGACCTAACACTTTTGTGGCAG 276
|| | | |||| | |||| || ||||||||||| ||||||||||| ||||||||| |||
Sbjct 135 CAACCAG-TTCAGTATAGCAGACTTCGGTTGTGCGAGTGGACCTAATACTTTTGTGTCAG 193
Query 277 TTCAAAACATAATAGATGCGGTAGAAGAAAAGTACCGTAGAGAAACCGGACAAAACCCGG 336
| ||||||||||| ||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 194 TCCAAAACATAATTGATGCGGTAGAAGAAAAATACCGTAGAGAAACCGGACAAAACCCGG 253
Query 337 CAGATAACATCGAGTTCCAAGTCCTCTTCAATGA-TTTCAGCCTCAATGATTTCAACACT 395
|| | |||||| |||||||||| ||||||||||| ||| ||| |||| ||||||||||||
Sbjct 254 CAAACAACATCAAGTTCCAAGTTCTCTTCAATGACTTT-AGCATCAACGATTTCAACACT 312
Query 396 CTCTTCCAGACACTTCCACCCGGAAGAAGATACT-TCAGCGCTGG 439
||||| || |||||||| | || |||||||||| | ||||||||
Sbjct 313 CTCTTTCAAACACTTCCTCAAGGGAGAAGATACTAT-AGCGCTGG 356
Score = 176 bits (95), Expect = 3e-43
Identities = 132/149 (89%), Gaps = 5/149 (3%)
Strand=Plus/Plus
Query 538 GGATTATGGACCGCGACTCTCCCTTGTGGAACAAAGACATGCAGTGCACGGGGTTCAACC 597
|||| |||||||| ||||||||||||||||||||||||||||||||||| |||| |||||
Sbjct 386 GGATCATGGACCGTGACTCTCCCTTGTGGAACAAAGACATGCAGTGCACCGGGTACAACC 445
Query 598 CCGCTGTCAAGAAAGCGTATCTTGACCAGTACTCTATCGACACCAA-AATTCTTTTAGAT 656
|| || || ||||||||| |||| || ||||||||||||||||| || || || |||
Sbjct 446 AAGCGGT-AAAAAAGCGTATTTTGATCAATACTCTATCGACACCAATAA-TC--TTGGAT 501
Query 657 GCTAGAGCTGAAGAGCTCGTGCCCGGGGG 685
|||||||||||||||||||||||||||||
Sbjct 502 GCTAGAGCTGAAGAGCTCGTGCCCGGGGG 530
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 48568596258
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5