BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT3G07180.1
Length=2060
Score E
Sequences producing significant alignments: (Bits) Value
Ahg928388 jgi|Araly1|928388|scaffold_300845.1 3005 0.0
Ahg497414 jgi|Araly1|497414|fgenesh2_kg.384__2__AT3G07180.1 2791 0.0
> Ahg928388 jgi|Araly1|928388|scaffold_300845.1
Length=1843
Score = 3005 bits (1627), Expect = 0.0
Identities = 1771/1843 (96%), Gaps = 0/1843 (0%)
Strand=Plus/Plus
Query 16 TCTTCAACTCTCGGAAAGCACCGATGGAAGAAATCTCCGATCGTCTGAATCCGGGAATCA 75
||||||||||| ||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 1 TCTTCAACTCTAGGAAAGCACCGATGGAAGAAACCTCCGATCGTCTGAATCCGGGAATCA 60
Query 76 CGCCGGAGTTTGATCCGAAGACTATGCGTTCCACCAAACCTGGATTGAAGCGTCTCTTCA 135
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 61 CGCCGGAGTTTGATCCGAAGACTATGCGTTCTACCAAACCTGGATTGAAGCGTCTCTTCA 120
Query 136 TCACCAGCTCTGTACTCTTTTCATTCCTTTTAGGTGTGCCATTTCTGTGGAAGTCAGTGG 195
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 121 TCACCACCTCTGTACTCTTTTCATTCCTTTTAGGTGTGCCATTTCTGTGGAAGTCAGTGG 180
Query 196 AAATATATCGCTCTCCGTTACCGTTCCACGACATCGATTCTCTCTCCGATCAACTTGAGT 255
|||||||||||||||||||||||||||||||||||||||| |||||||||||| ||||||
Sbjct 181 AAATATATCGCTCTCCGTTACCGTTCCACGACATCGATTCGCTCTCCGATCAAATTGAGT 240
Query 256 CGACCCCTTTGCGTTTCCCTTGCAATTTCCACGCTGTTTTCGTCGGTTTTCGATCGACGG 315
| || |||||||||||||||||||||||||||||||| ||||||||||| | ||||||||
Sbjct 241 CTACTCCTTTGCGTTTCCCTTGCAATTTCCACGCTGTATTCGTCGGTTTCCCATCGACGG 300
Query 316 ATCCGGATAACTTACGATCTCAGATACAAGATGGTATAAATCAGTTAACTCATCAAAGTT 375
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 301 ATCCGGATAACTTACGATCTCAGATACAAGATGGTATAAATCAGTTAACTCATCAAAATT 360
Query 376 CACAATGTGGTTCTTGTGATTTTTCCCTCTCTGTCACCGTTCAAAACCGAGAAGACCAGT 435
||||||||||||| || ||||||||||||||||||||||||| |||||||| ||||| |
Sbjct 361 CACAATGTGGTTCCTGCAATTTTTCCCTCTCTGTCACCGTTCAGAACCGAGATGACCAAT 420
Query 436 GCTCTGATACACTTGCGCATAGTTCTACCACGTGCTCTTACCGTTGTGGAGTGATCAAAA 495
||||||| |||||||| || ||||||||||||||||||||||||||||||||||||||||
Sbjct 421 GCTCTGAAACACTTGCTCACAGTTCTACCACGTGCTCTTACCGTTGTGGAGTGATCAAAA 480
Query 496 GAAATGGTTTTAGTGTTGGATTGGATGATACAGTGGATGAGTCACTGAATGATGTGTTTA 555
|||||| |||| |||| ||| ||||||| |||||||||||||| |||||||||||||||
Sbjct 481 GAAATGTTTTTGGTGTGGGAGTGGATGAGACAGTGGATGAGTCTTTGAATGATGTGTTTA 540
Query 556 GTGGCTGTTCTGAGAACAGTGGGAAAATGTACAGTGTTGTCGTGGTGAATAAAGAGAATG 615
|||||||||||||| | ||||||||| ||||||||||||| |||||||||||||||||||
Sbjct 541 GTGGCTGTTCTGAGGAGAGTGGGAAAGTGTACAGTGTTGTTGTGGTGAATAAAGAGAATG 600
Query 616 CCAATGGTGGAGATGAGGTAAAAGCTGTGGTGGGGAAGCGAAGGCATGCGTGGATTGTGG 675
|||||||||| ||||||||||||||||| ||||| |||||||||||||||||||||||||
Sbjct 601 CCAATGGTGGGGATGAGGTAAAAGCTGTTGTGGGTAAGCGAAGGCATGCGTGGATTGTGG 660
Query 676 GCAATGGATTAGAAGAGAGGTATGGTGACATTGTGGCTAGAGTTTCTGAGATTTTCGTTC 735
| |||||||||||||| || ||||||||||| |||||||||||||||||||||||||||
Sbjct 661 GGAATGGATTAGAAGAAAGATATGGTGACATGGTGGCTAGAGTTTCTGAGATTTTCGTTA 720
Query 736 AGGTGTTTATGAATGGTGGAAGAGAAGAAGACTCAATTCAGGGAGAGTTTATGCCTGTGG 795
|||||||||||| ||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 721 AGGTGTTTATGACTGGTGGAAGAGAAGAAGACTCAATTCAGGGAGAGTTTATGCCTGTTG 780
Query 796 GATCAGATGGGAAGATTGTTCTTTCGTTCAGTCTTTTAAATTCCAATCCACGTGATTGGG 855
|||||||||||| ||||||||||||||||||||| |||||||| ||||||||||||||||
Sbjct 781 GATCAGATGGGAGGATTGTTCTTTCGTTCAGTCTATTAAATTCTAATCCACGTGATTGGG 840
Query 856 TCTACGATTGGGACTTTCAGAGGATAGATGAGGCATTGTTGGCTCCGGTGACTAAGGCCT 915
||||||||||||||||||| |||||||| ||||||||||||||||| |||||||||||||
Sbjct 841 TCTACGATTGGGACTTTCAAAGGATAGACGAGGCATTGTTGGCTCCAGTGACTAAGGCCT 900
Query 916 TAGCACCTATAGCTAATATAACTGTTGAAAGTCAGGTTTTGTATCATACACCGAAGTCTT 975
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 901 TAGCACCTATAGCTAATATAAGTGTTGAAAGTCAGGTTTTGTATCATACACCGAAGTCTT 960
Query 976 CGTTTTCATCTTGGGATAAAAAACTTCAGAGCTACATCTTTAGGACCAGTGATCTTCCGT 1035
| |||||||||||||||||||||||||||||||||||||||||||| ||||||||||| |
Sbjct 961 CTTTTTCATCTTGGGATAAAAAACTTCAGAGCTACATCTTTAGGACTAGTGATCTTCCCT 1020
Query 1036 TTTTTGTGAATTCGAACGAATGGCACTTGGATACTTCTGCTGGAGCCAGTGGACGGTCCA 1095
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1021 TTTTCGTGAATTCGAACGAATGGCACTTGGATACTTCTGCTGGAGCCAGTGGACGGTCCA 1080
Query 1096 AAATACTGCAATTTGTGGTATATATTCCATCCGGAAAAGAATGCCCTCTTCTTTTGCAGT 1155
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1081 AAATACTGCAATTTGTGGTATATATTCCATCCGGAAAAGAATGCCCTCTTCTTTTGCAGT 1140
Query 1156 TGCCAAATGTGGAGATATCTAAAACAAATGGCTTCATATCTCCGATGTGGGGTGGTGTGA 1215
| ||||||| |||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 1141 TACCAAATGGGGAGATATCTAAAACAAATGGCTTCATATCTCCGATGTGGGGCGGTGTGA 1200
Query 1216 TTGTGTGGAACCCGGGAAATTGTGACAAGGACTCTGAAAGCCCAAGTAGGAACACGATAT 1275
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 1201 TTGTGTGGAACCCGGGAAATTGTAACAAGGACTCTGAAAGCCCAAGTAGGAACACGATAT 1260
Query 1276 CGCTTCAGGATCTCGAGCAAATAGTCGAAATTTTCTTGGGACAATTCCGACAACTATTTG 1335
||| ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 1261 CGCCTCAGGATCTCGAGCAAATAGTTGAAATTTTCTTGGGACAATTCCGACAACTATTTG 1320
Query 1336 GCTTTAAGTCTGAAGCCAAATATACCACTGGATTGGGCACTTACAAGATTTTAACTAGTG 1395
|||||||||||||||||| ||||||| ||||||||||||||||||||||||| ||||||
Sbjct 1321 GCTTTAAGTCTGAAGCCATTTATACCAGTGGATTGGGCACTTACAAGATTTTACCTAGTG 1380
Query 1396 AAAGAGGCTTTACAGAGTGGGAATTGGATGTCTTGTCACGGAAGCACACTTGCTTCAATC 1455
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 1381 AAAGAGGCTTTACAGAGTGGGAATTGGATGTCTTGTCACGGAAGCACACATGCTTCAATC 1440
Query 1456 TCCATTCTTGTTCAACAACCCTCGGTTCTCTCTCTAGATTGGTTCGGTCACTCCCCAGGA 1515
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1441 TCCATTCTTGTTCAACAACCCTCGGTTCTCTCTCTAGATTGGTTCGGTCACTCCCCAGGA 1500
Query 1516 TGATTATCAAGGATGAAATTGGAGAACAAGTGAAATATTCTCTGAAAGCAGCAAAGTTGG 1575
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1501 TGATTATCAAGGATGAAATTGGAGAACAAGTGAAATATTCTCTGAAAGCAGCAAAGTTGG 1560
Query 1576 CTCAGTCTAATGCTTCTTTGGGAGGATACAGTTCTTCAGCCAGTTCATCAAGAGAAGCAA 1635
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1561 CTCAGTCTAATGCTTCTTTGGGAGGATACAGTTCTTCAGCCAGTTCATCAAGAGAAGCAA 1620
Query 1636 GGTCTTTAGCCGAGAATGCCTTCTTCCATCCATCCATCATGTCCGTCAGCTACTTCTCTT 1695
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1621 GGTCTCTAGCCGAGAATGCCTTCTTCCATCCATCCATCATGTCCGTCAGCTACTTCTCTT 1680
Query 1696 ATGAACATTGCTTTGCAGTGTACTCACCGTTTTTCCTGCCGGTGGTAGGTCACGTTGTCC 1755
|||||||||||||||||||||||||||| ||||||||||| |||| ||| ||||||||||
Sbjct 1681 ATGAACATTGCTTTGCAGTGTACTCACCATTTTTCCTGCCCGTGGCAGGACACGTTGTCC 1740
Query 1756 TTGCAGCAGTACGAGAATGGAAGAGATACAAACAAGAGAAGGCAAAGTACTTGACATGGC 1815
|||||||| |||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1741 TTGCAGCACTACGAGAATGGAGGAGATACAAACAAGAGAAGGCAAAGTACTTGACATGGC 1800
Query 1816 TAACCAGAAAGAAGACGACCTAGGACTCTTTAGCATACTACAG 1858
||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1801 TAACCAGAAAGAAGACGGCCTAGGACTCTTTAGCATACTACAG 1843
> Ahg497414 jgi|Araly1|497414|fgenesh2_kg.384__2__AT3G07180.1
Length=1805
Score = 2791 bits (1511), Expect = 0.0
Identities = 1670/1749 (95%), Gaps = 2/1749 (0%)
Strand=Plus/Plus
Query 126 CGTCTCTTCATCACCAGCTCTGTACTCTTTTCATTCCTTTTAGGTGTGCCATTTCTGTGG 185
|||| ||||||||||| |||||| ||||| ||||||||||||||||||||||||||||||
Sbjct 58 CGTCCCTTCATCACCACCTCTGTGCTCTTCTCATTCCTTTTAGGTGTGCCATTTCTGTGG 117
Query 186 AAGTCAGTGGAAATATATCGCTCTCCGTTACCGTTCCACGACATCGATTCTCTCTCCGAT 245
|||||||||||||||||||||||||||||||||||||||||||||||||| || ||||||
Sbjct 118 AAGTCAGTGGAAATATATCGCTCTCCGTTACCGTTCCACGACATCGATTCGCTTTCCGAT 177
Query 246 CAACTTGAGTCGACCCCTTTGCGTTTCCCTTGCAATTTCCACGCTGTTTTCGTCGGTTTT 305
||| ||||||| || |||||||||||||||||||||||||||||||| |||||||||||
Sbjct 178 CAAATTGAGTCTACTCCTTTGCGTTTCCCTTGCAATTTCCACGCTGTATTCGTCGGTTTC 237
Query 306 CGATCGACGGATCCGGATAACTTACGATCTCAGATACAAGATGGTATAAATCAGTTAACT 365
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 238 CGATCGACGGATCCGGATAACTTGCGATCTCAGATACAAGATGGTATAAATCAGTTAACT 297
Query 366 CATCAAAGTTCACAATGTGGTTCTTGTGATTTTTCCCTCTCTGTCACCGTTCAAAACCGA 425
||||||| ||||||||||||||| || ||||||||||||||||||||||||||||||||
Sbjct 298 CATCAAAATTCACAATGTGGTTCCTGCAATTTTTCCCTCTCTGTCACCGTTCAAAACCGA 357
Query 426 GAAGACCAGTGCTCTGATACACTTGCGCATAGTTCTACCACGTGCTCTTACCGTTGTGGA 485
|| |||||||||||||| ||||||||||| ||||||||||||||||| ||||||||||||
Sbjct 358 GATGACCAGTGCTCTGAAACACTTGCGCACAGTTCTACCACGTGCTCATACCGTTGTGGA 417
Query 486 GTGATCAAAAGAAATGGTTTTAGTGTTGGATTGGATGATACAGTGGATGAGTCACTGAAT 545
|||||||||||||||| |||| |||||||| |||||||||||||||||||||||||||||
Sbjct 418 GTGATCAAAAGAAATGTTTTTGGTGTTGGAGTGGATGATACAGTGGATGAGTCACTGAAT 477
Query 546 GATGTGTTTAGTGGCTGTTCTGAGAACAGTGGGAAAATGTACAGTGTTGTCGTGGTGAAT 605
|||||||| ||||||||||||||| | ||||||||||||||||||||||| ||||||||
Sbjct 478 GATGTGTTCAGTGGCTGTTCTGAGGAGAGTGGGAAAATGTACAGTGTTGTTGTGGTGAAC 537
Query 606 AAAGAGAATGCCAATGGTGGAGATGAGGTAAAAGCTGTGGTGGGGAAGCGAAGGCATGCG 665
|||||||||||||||||| |||||||||||||||||||||||| ||| |||||||||||
Sbjct 538 AAAGAGAATGCCAATGGTAAAGATGAGGTAAAAGCTGTGGTGGGCAAGGGAAGGCATGCG 597
Query 666 TGGATTGTGGGCAATGGATTAGAAGAGAGGTATGGTGACATTGTGGCTAGAGTTTCTGAG 725
||||||||||| |||||||||||||| || ||||||||||| ||||||||||||||||||
Sbjct 598 TGGATTGTGGGGAATGGATTAGAAGAAAGATATGGTGACATGGTGGCTAGAGTTTCTGAG 657
Query 726 ATTTTCGTTCAGGTGTTTATGAATGGTGGAAGAGAAGAAGACTCAATTCAGGGAGAGTTT 785
||||||||| || ||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 658 ATTTTCGTTAAGATGTTTATGAATGGTGGAAGAGAAGAAGACTCAATTCAAGGAGAGTTT 717
Query 786 ATGCCTGTGGGATCAGATGGGAAGATTGTTCTTTCGTTCAGTCTTTTAAATTCCAATCCA 845
|||||||||||||||||||||| ||||||||||||||||||||| |||||||| ||||||
Sbjct 718 ATGCCTGTGGGATCAGATGGGAGGATTGTTCTTTCGTTCAGTCTATTAAATTCTAATCCA 777
Query 846 CGTGATTGGGTCTACGATTGGGACTTTCAGAGGATAGATGAGGCATTGTTGGCTCCGGTG 905
||||||||||||||||||||||||||||| |||||||| ||||||||||||||||| |||
Sbjct 778 CGTGATTGGGTCTACGATTGGGACTTTCAAAGGATAGACGAGGCATTGTTGGCTCCCGTG 837
Query 906 ACTAAGGCCTTAGCACCTATAGCTAATATAACTGTTGAAAGTCAGGTTTTGTATCATACA 965
||||| ||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 838 ACTAAAGCCTTAGCACCTATAGCTAATATAAGTGTTGAAAGTCAGGTTTTGTATCATACA 897
Query 966 CCGAAGTCTTCGTTTTCATCTTGGGATAAAAAACTTCAGAGCTACATCTTTAGGACCAGT 1025
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 898 CCGAAGTCTTCGTTTTCATCTTGGGATAAAAAACTTCAGAGCTACATCTTTAGGACCAGT 957
Query 1026 GATCTTCCGTTTTTTGTGAATTCGAACGAATGGCACTTGGATACTTCTGCTGGAGCCAGT 1085
| |||||| ||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 958 GCTCTTCCCTTTTTCGTGAATTCGAACGAATGGCACTTGGATACTTCTGCTGGAGCCAGT 1017
Query 1086 GGACGGTCCAAAATACTGCAATTTGTGGTATATATTCCATCCGGAAAAGAATGCCCTCTT 1145
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 1018 GGACGGTCCAAAATACTGCAATTTGTGGTATATATTCCGTCCGGAAAAGAATGCCCTCTT 1077
Query 1146 CTTTTGCAGTTGCCAAATGTGGAGATATCTAAAACAAATGGCTTCATATCTCCGATGTGG 1205
||||||||||||||||||| |||||||||||||||||||||||||||||||||||| |||
Sbjct 1078 CTTTTGCAGTTGCCAAATGGGGAGATATCTAAAACAAATGGCTTCATATCTCCGATATGG 1137
Query 1206 GGTGGTGTGATTGTGTGGAACCCGGGAAATTGTGACAAGGACTCTGAAAGCCCAAGTAGG 1265
|| |||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 1138 GGCGGTGTGATTGTGTGGAACCCGGGAAATTGTGACAAGGACTCTGAAAGCTCAAGTAGG 1197
Query 1266 AACACGATATCGCTTCAGGATCTCGAGCAAATAGTCGAAATTTTCTTGGGACAATTCCGA 1325
||||||||||||| ||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 1198 AACACGATATCGCCTCAGGATCTCGAGCAAATAGTTGAAATTTTCTTGGGACAATTCCGA 1257
Query 1326 CAACTATTTGGCTTTAAGTCTGAAGCCAAATATACCACTGGATTGGGCACTTACAAGATT 1385
||||||||||||||||||||||||||| ||||||| ||||||||||||||||||||||
Sbjct 1258 CAACTATTTGGCTTTAAGTCTGAAGCCTTTTATACCAGTGGATTGGGCACTTACAAGATT 1317
Query 1386 TTAACTAGTGAAAGAGGCTTTACAGAGTGGGAATTGGATGTCTTGTC-ACGGAAGCACAC 1444
|| ||||||| ||||||||||||||||||||||||||||||||||| || |||||||||
Sbjct 1318 CTACCTAGTGACAGAGGCTTTACAGAGTGGGAATTGGATGTCTTGTCAAC-GAAGCACAC 1376
Query 1445 TTGCTTCAATCTCCATTCTTGTTCAACAACCCTCGGTTCTCTCTCTAGATTGGTTCGGTC 1504
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1377 ATGCTTCAATCTCCATTCTTGTTCAACAACCCTCGGTTCTCTCTCTAGATTGGTTCGGTC 1436
Query 1505 ACTCCCCAGGATGATTATCAAGGATGAAATTGGAGAACAAGTGAAATATTCTCTGAAAGC 1564
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1437 ACTCCCCAGGATGATTATCAAGGATGAAATTGGAGAACAAGTGAAATATTCTCTGAAAGC 1496
Query 1565 AGCAAAGTTGGCTCAGTCTAATGCTTCTTTGGGAGGATACAGTTCTTCAGCCAGTTCATC 1624
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 1497 AGCAAAGTTGGCTCAGTCTAATGCTTCTTTGGGAGAATACAGTTCTTCAGCCAGTTCATC 1556
Query 1625 AAGAGAAGCAAGGTCTTTAGCCGAGAATGCCTTCTTCCATCCATCCATCATGTCCGTCAG 1684
|||||||||||||||| |||||||||||||||||||||||||||| ||||||||||||||
Sbjct 1557 AAGAGAAGCAAGGTCTCTAGCCGAGAATGCCTTCTTCCATCCATCTATCATGTCCGTCAG 1616
Query 1685 CTACTTCTCTTATGAACATTGCTTTGCAGTGTACTCACCGTTTTTCCTGCCGGTGGTAGG 1744
||||||||||| |||||||||||||||||||||||| |||||||||||||| |||| |||
Sbjct 1617 CTACTTCTCTTCTGAACATTGCTTTGCAGTGTACTCGCCGTTTTTCCTGCCCGTGGCAGG 1676
Query 1745 TCACGTTGTCCTTGCAGCAGTACGAGAATGGAAGAGATACAAACAAGAGAAGGCAAAGTA 1804
|||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1677 ACACGTTGTCCTTGCAGCACTACGAGAATGGAAGAGATACAAACAAGAGAAGGCAAAGTA 1736
Query 1805 CTTGACATGGCTAACCAGAAAGAAGACGACCTAGGACTCTTTAGCATACTACAGATTCTC 1864
|||||||||||||||||||||||||||| ||||||||||||||||||| |||| ||||||
Sbjct 1737 CTTGACATGGCTAACCAGAAAGAAGACGGCCTAGGACTCTTTAGCATATTACATATTCTC 1796
Query 1865 TGTAACTTT 1873
|||||||||
Sbjct 1797 TGTAACTTT 1805
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 77475558330
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5