BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT3G10200.1
Length=1958
Score E
Sequences producing significant alignments: (Bits) Value
Ahg928709 jgi|Araly1|928709|scaffold_301166.1 2145 0.0
Ahg487185 jgi|Araly1|487185|fgenesh2_kg.6__319__AT5G04060.1 1489 0.0
> Ahg928709 jgi|Araly1|928709|scaffold_301166.1
Length=1776
Score = 2145 bits (1161), Expect = 0.0
Identities = 1284/1344 (96%), Gaps = 5/1344 (0%)
Strand=Plus/Plus
Query 468 TTAAA-GGTGGCCAAAATTGGGTCCATGAGCAAGGTCAGTTTTGGTGGTTCCCTGGTGGT 526
||||| ||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 436 TTAAAGGGTGGCCAAAATTGGGTGCATGAGCAAGGTCAGTTTTGGTGGTTCCCTGGTGGT 495
Query 527 GGTACTCATTTTAAGCATGGAGCTGCAGAATACATCCAGAGGTTGGGGAATATGATGACT 586
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct 496 GGTACTCATTTTAAGCATGGAGCTGCAGAATATATCCAGAGGTTGGGGAATATGATGACT 555
Query 587 AATGAAACAGGTGACTTGCGTTCAGCTGGGGTGGTACAAGTTCTTGATGTTGGCTGTGGA 646
||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 556 AATGAAACAGGTGACTTTCGTTCAGCTGGGGTGGTACAAGTTCTTGATGTTGGCTGTGGC 615
Query 647 GTTGCGAGCTTTGCGGCTTATCTTCTTCCCTTAGGTATACAGACAATTTCATTTGCTCCC 706
|||| ||||||||| |||||||||||||| ||||||||||||||||| ||||||||||||
Sbjct 616 GTTGTGAGCTTTGCCGCTTATCTTCTTCCTTTAGGTATACAGACAATGTCATTTGCTCCC 675
Query 707 AAAGATGGTCATGAAAACCAGATTCAGTTTGCATTGGAGAGAGGCATTGGTGCAATGATA 766
|||||||||||||||||||||||||||||||||||||| |||||||| ||||| ||||||
Sbjct 676 AAAGATGGTCATGAAAACCAGATTCAGTTTGCATTGGAAAGAGGCATCGGTGCTATGATA 735
Query 767 TCCGCGGTTGCCACCAAACAATTGCCATATCCTGCAGCCTCGTTTGAGATGGTTCATTGT 826
||||||||||||||||||||| |||| |||||||||||||||||||||||||||||||||
Sbjct 736 TCCGCGGTTGCCACCAAACAAATGCCTTATCCTGCAGCCTCGTTTGAGATGGTTCATTGT 795
Query 827 TCGAGATGTCGTGTTGACTGGCATACAAATGATGGCATCTTACTTAAAGAAGTTCATCGT 886
||||||||||||||||| |||||| ||||||||||||||||||| |||||||||||||||
Sbjct 796 TCGAGATGTCGTGTTGATTGGCATGCAAATGATGGCATCTTACTAAAAGAAGTTCATCGT 855
Query 887 CTTCTCCGACCAAATGGATTCTTCGTGTATTCATCACCACCAGCATATAGAAAGGATAAA 946
||||||||||||||||||||||| ||||||||||| |||||||| |||||||| ||||||
Sbjct 856 CTTCTCCGACCAAATGGATTCTTTGTGTATTCATCGCCACCAGCTTATAGAAATGATAAA 915
Query 947 GAGTATCCTATGATTTGGGATAAGTTGGTCAATCTAACTAGTGCAATGTGCTGGAAGCTT 1006
|||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 916 GAGTATCCTATGATCTGGGATAAGTTGGTCAATCTAACTAGTGCAATGTGCTGGAAGCTC 975
Query 1007 ATTTCCCGGAAGGTACAGACTGCAATATGGATTAAAGAAGAGAAGGAGGTTTGTCTTAAG 1066
|||||||||||||||||||||||||||||||||||||| ||||| ||||| ||||||| |
Sbjct 976 ATTTCCCGGAAGGTACAGACTGCAATATGGATTAAAGACGAGAATGAGGTGTGTCTTAGG 1035
Query 1067 CAGAAAGCAGAGCTAAAGCTAATAAGTTTATGTGATGTGGAAGATGTTTTGAAACCATCA 1126
||||| |||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1036 CAGAATGCAGAGCTAAAGCTCATAAGTTTATGTGATGTGGAAGATGTTTTGAAACCATCA 1095
Query 1127 TGGAAAGTTCCTCTTAAAGATTGTGTGCAAATCAGTGGACAAACAGAAGAGAGACCCTCT 1186
||||||||| |||| | |||||||||||||||||||||||||||||||||||||||||||
Sbjct 1096 TGGAAAGTTACTCTGAGAGATTGTGTGCAAATCAGTGGACAAACAGAAGAGAGACCCTCT 1155
Query 1187 TCTTTAGCTGAACGTCTTTCTGCATATCCAGCAACTCTAAGAAAAATAGGGATCAGTGAA 1246
||||||||||||||||||||||| ||||| | |||||||||||||||||||||||||||
Sbjct 1156 TCTTTAGCTGAACGTCTTTCTGCGTATCCGGGGACTCTAAGAAAAATAGGGATCAGTGAA 1215
Query 1247 GATGAATATACATCAGATACAGTGTTCTGGAGAGAACAAGTTAATCATTACTGGCGGTTA 1306
||||||||||||||||||||||| | ||||||||||||||||||||| || |||||||||
Sbjct 1216 GATGAATATACATCAGATACAGTCTACTGGAGAGAACAAGTTAATCACTATTGGCGGTTA 1275
Query 1307 ATGAATGTCAATGAGACTGAAGTAC-GGAATGTGATGGACATGAACGCATTCATTGGTGG 1365
||||||||||||||||||||||| | |||||| |||||||||||||||||||||||||||
Sbjct 1276 ATGAATGTCAATGAGACTGAAGT-CAGGAATGCGATGGACATGAACGCATTCATTGGTGG 1334
Query 1366 TTTTGCTGCAGCTATGAATTCGTATCCTGTTTGGGTAATGAACATAGTACCTGCAACCAT 1425
||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct 1335 GTTTGCTGCAGCTATGAATTCGTATCCTGTTTGGGTAATGAACATAGTACCTGCTACCAT 1394
Query 1426 GAATGATACCTTGTCTGGAATTTTCGAGAGAGGCTTAAATGGCGCTTTCCATGATTGGTG 1485
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 1395 GAATGATACCTTGTCTGGAATTTTCGAGAGAGGCTTAAATGGAGCTTTCCATGATTGGTG 1454
Query 1486 TGAGGCGTTCTCAACATATCCACGCACGTATGATTTGGTGCATTCCGATCATGTCTTCTC 1545
||||||||||||||||||||| ||||||||||||||| ||||||||||||||||||||||
Sbjct 1455 TGAGGCGTTCTCAACATATCCTCGCACGTATGATTTGCTGCATTCCGATCATGTCTTCTC 1514
Query 1546 TCACTACAACAAAAGCTACGGAGATGGTTGTTTGCTAGAGGATATCATGCTTGAGATGGA 1605
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1515 TCATTACAACAAAAGCTACGGAGATGGTTGTTTGCTAGAGGATATCATGCTTGAGATGGA 1574
Query 1606 CCGCATAGTTCGCCCTCAGGGGTTTGTCATAATAAGGGACGAGGAATATATCATCTCAAG 1665
||||||||||||||||||||||||| ||||||||||||||||||||| ||||||||||||
Sbjct 1575 CCGCATAGTTCGCCCTCAGGGGTTTATCATAATAAGGGACGAGGAATCTATCATCTCAAG 1634
Query 1666 GATCCGAGGCTTGGCTCCAAAATTCTTGTGGGAAGTGGAAACTCATGAGCTTGAAAACAA 1725
|||||||| ||||||||| ||||| |||||||||||||||||||||||||||||||||||
Sbjct 1635 GATCCGAGACTTGGCTCCTAAATTATTGTGGGAAGTGGAAACTCATGAGCTTGAAAACAA 1694
Query 1726 AGACAAGAAGATAACAGAGTCTGTTTTGTTTTGCAGAAAGAGATTCTGGGCAATCATTTG 1785
|||||||||||| |||||| ||||||||||||||||||||||||||||||| ||||||||
Sbjct 1695 AGACAAGAAGATGACAGAGACTGTTTTGTTTTGCAGAAAGAGATTCTGGGCTATCATTTG 1754
Query 1786 AAATAGACTCACTATTTTTAGCTC 1809
|| ||||||| |||||||| ||||
Sbjct 1755 AA-TAGACTCGCTATTTTT-GCTC 1776
Score = 595 bits (322), Expect = 3e-169
Identities = 394/427 (92%), Gaps = 12/427 (3%)
Strand=Plus/Plus
Query 1 GACAGAAACAATGAGAGGTTCTGTAATTGGTGCAGAGAGATCCGGGCAGACGATAATGGT 60
||||||||||||| |||||||||| ||||||||| |||||| |||||||||||||||||
Sbjct 21 GACAGAAACAATGGGAGGTTCTGTGTTTGGTGCAGCGAGATCAGGGCAGACGATAATGGT 80
Query 61 GGCTCTCGTTCTTATGGTTGGATCATTTTACACTGGCTCTCTCTTCGGAACCAACCAACC 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 81 TGCTCTCGTTCTTATGGTTGGATCATTTTACACTGGCTCTCTCTTCGGAACCAACCAACC 140
Query 121 CATTTACGTCTCTCATCCTTCTTCTCACTCTGCTTCTTCCAAATTTGCAAACAAAATTGA 180
||||||||||||||| |||||||||||| |||||||||||||||||||||||||| | |
Sbjct 141 CATTTACGTCTCTCAACCTTCTTCTCACCCTGCTTCTTCCAAATTTGCAAACAAAGTCGC 200
Query 181 GCTTACTTATAGAAGATTACCACTAGTCATACCTGAATCTGGGATGAATGTGTGTCCACT 240
|||||||||||| |||||||||||||||||||||||| |||||||||||||||||||| |
Sbjct 201 GCTTACTTATAGGAGATTACCACTAGTCATACCTGAAACTGGGATGAATGTGTGTCCATT 260
Query 241 GGAGTTCAATGAGTATATACCTTGTCACAATGTCACCTATGTGCATCAGCTGCTACCAAG 300
|||||||||||||||||| ||||||||||||||||| ||||||||||||||||| |||||
Sbjct 261 GGAGTTCAATGAGTATATCCCTTGTCACAATGTCACTTATGTGCATCAGCTGCTTCCAAG 320
Query 301 CTTGAATCTTTCTAGAAGAGAAGACCTCGAAAGGCATTGCCCGCCACTCGAGCACCGCCT 360
|||||||||||||||||||||||| |||||||||||||||||||||||||||||| |
Sbjct 321 CTTGAATCTTTCTAGAAGAGAAGAGCTCGAAAGGCATTGCCCGCCACTCGAGCAC----T 376
Query 361 TTTTTGCTTGGTACCTCCGCCAAATGATTATAAGATACCTATTAGATGGCCAACCAGTAG 420
| | | ||||||| ||||||||||||||||||||||| | |||||||||||||||
Sbjct 377 T----G---G-TACCTCCACCAAATGATTATAAGATACCTATAAAATGGCCAACCAGTAG 428
Query 421 AGACTAT 427
|||||||
Sbjct 429 AGACTAT 435
> Ahg487185 jgi|Araly1|487185|fgenesh2_kg.6__319__AT5G04060.1
Length=1998
Score = 1489 bits (806), Expect = 0.0
Identities = 1487/1806 (82%), Gaps = 86/1806 (5%)
Strand=Plus/Plus
Query 38 AGATCCGGG-CAGACGATAATGGTGGCTCTCGTTCTTATGGTTGGATCATTTTACACTGG 96
|||| |||| |||||||||||||||||||| ||||| |||||||||||||||||| | ||
Sbjct 56 AGAT-CGGGACAGACGATAATGGTGGCTCTGGTTCTAATGGTTGGATCATTTTACGCAGG 114
Query 97 CTCTCTCTTCGGAACCAA----CC-A-ACCCATTT----A-CGTC-TC-TCATCC--TTC 141
|||| ||||||||| ||| || | ||||| | | || || || ||| |||
Sbjct 115 CTCTATCTTCGGAAACAATTCTCCTATTTACATTTCCCAACCTTCTTCTTCTTCCAATTC 174
Query 142 TTC-TC-AC-TCTGC-T-----TCT--T-C--CAAA-TTTGCAAACAAAATTGAGCTTAC 186
||| || | ||| | | ||| | | |||| ||||| ||||| || ||||||||
Sbjct 175 TTCTTCTTCTTCTCCATCACAATCTGGTCCATCAAATTTTGCTAACAAGATAGAGCTTAC 234
Query 187 TTA-TAGAAGATTACCACTAG--TC-ATACCTG-AATCTGGGATGAATGTGTGTCCACTG 241
||| || ||| || || | || || |||| | |||| ||||||| |||||| ||
Sbjct 235 TTACCAG-AGA--ACTTCT-GTTTCGATCCCTGCGA-GTGGGGTGAATGTTTGTCCATTG 289
Query 242 GAGTTCAATGAGTAT-ATACCTTGTCACAATGTCACCTATGTGCATCAGCTGCTACCAAG 300
|||||||||||||| || ||||||||||||||||| |||||||| ||||| || |||||
Sbjct 290 AAGTTCAATGAGTATAAT-CCTTGTCACAATGTCACTTATGTGCAACAGCTTCTCCCAAG 348
Query 301 CTTGAATCTTTCTAGAAGAGAAGACCTCGAAAGGCATTGCCCGCCACTCGAGCACCGCCT 360
|||||||||||||||||||||||| || || || || || |||||||| || || || |
Sbjct 349 CTTGAATCTTTCTAGAAGAGAAGAGCTTGAGAGACACTGTCCGCCACTTGAACAGCG-TT 407
Query 361 T-TTTTGCTTGGTACCTCCGCCAAATGATTATAAGATACCTATTA-GATGGCCAACCAGT 418
| ||||| ||||| ||||||||||| |||||||||||||| | || | |||||||| ||
Sbjct 408 TGTTTTGTTTGGTGCCTCCGCCAAAAGATTATAAGATACC-AATACGCTGGCCAACTAGC 466
Query 419 AGAGACTATGTATGGAGAAGTAATGTGAACCATACACATCTTGCTCAAGTTAAAGGTGGC 478
||||| |||||||||||||| |||||||| ||||| ||||||||| |||| ||||| |||
Sbjct 467 AGAGATTATGTATGGAGAAGCAATGTGAATCATACGCATCTTGCTGAAGTGAAAGGCGGC 526
Query 479 CAAAATTGGGTCCATGAGCAAGGTCAGTTTTGGTGGTTCCCTGGTGGTGGTACTCATTTT 538
|| |||||||| ||||| |||||||| || ||||||||||||||||| |||||||||||
Sbjct 527 CAGAATTGGGTGCATGAACAAGGTCAATTATGGTGGTTCCCTGGTGGCGGTACTCATTTC 586
Query 539 AAGCATGGAGCTGCAGAATACATCCAGAGGTTGGGGAATATGATGACTAATGAAACAGGT 598
|| || |||||| ||||||||||||||||||| || || |||| |||||||||||||||
Sbjct 587 AAACACGGAGCTCCAGAATACATCCAGAGGTTAGGAAACATGACAACTAATGAAACAGGT 646
Query 599 GA-CTTGCGTTCAGCTGGGGTGGTACAAGTTCTTGATGTTGGCTGTGGAGTTGCGAGCTT 657
|| ||| ||||||||||| || | |||||||||||||||||| ||||||||||| |||||
Sbjct 647 GACCTT-CGTTCAGCTGGTGTTGAACAAGTTCTTGATGTTGGATGTGGAGTTGCTAGCTT 705
Query 658 TGCGGCTTATCTTCTTCCCTTAGGTATACAGACAATTTCATTTGCTCCC-AAAGATGGTC 716
||| |||||||||||||| ||||||||| ||||||| || ||||| ||| ||||||||||
Sbjct 706 TGCAGCTTATCTTCTTCCTTTAGGTATAAAGACAATGTCTTTTGC-CCCTAAAGATGGTC 764
Query 717 ATGAAAACCAGATTCAGTTTGCATTGGAGAGAGGCATTGGTGCAATGATATCCGCGGTTG 776
|||||||||| || |||||||| ||||||||||| || || |||||||| || || | |
Sbjct 765 ATGAAAACCAAATCCAGTTTGCGTTGGAGAGAGGAATCGGCGCAATGATCTCTGCCATAG 824
Query 777 CCACCAAACAATTGCCATATCCTGCAGCCTCGTTTGAGATGGTTCATTGTTCGAGATGTC 836
||||||||||| |||||||||| || ||||||||||| |||||||| ||||| |||||||
Sbjct 825 CCACCAAACAAATGCCATATCCCGCGGCCTCGTTTGATATGGTTCACTGTTCAAGATGTC 884
Query 837 GTGTTGACTGGCAT-ACAAATGATGGCATCTT-ACTTAAAGAAGTTCATCGTCTTCTCCG 894
||||||| |||||| | ||||||||| ||||| | ||||||| ||| |||||||||||||
Sbjct 885 GTGTTGATTGGCATGA-AAATGATGGTATCTTGA-TTAAAGAGGTTAATCGTCTTCTCCG 942
Query 895 ACCAAATGGATTCTTCGTGTATTCATCACCACCAGCATATAGAAAGGATAAAGAGTAT-C 953
||| ||||||| ||| || |||||| ||||||| ||||| |||||||||||||| | | |
Sbjct 943 ACCCAATGGATACTTTGTTTATTCAGCACCACCTGCATACAGAAAGGATAAAGA-TTTCC 1001
Query 954 CTATGATTTGGGATAAGTTGGTCAATCTAACTAGTGCAATGTGCTGGAAGCTTATTTCCC 1013
||||||||||||||||||||||||||||||||| ||||||||||||||||| |||||||
Sbjct 1002 CTATGATTTGGGATAAGTTGGTCAATCTAACTACCGCAATGTGCTGGAAGCTCATTTCCC 1061
Query 1014 GGAAGGTACAGACTGCAATATGGATTAAAGAAGA-GAAGGAGGTTTGTCTTAAGCAGAAA 1072
| ||||||||||||||||||||| |||||||||| || | ||| ||| |||| | ||||
Sbjct 1062 GAAAGGTACAGACTGCAATATGGGTTAAAGAAGATGACG-AGGCTTGCCTTAGGAAGAAT 1120
Query 1073 GCAGAGCTAAAGCTAATAAGTTTATGTGATGTGGAAGATGTTTTGAAACCATCATGGAAA 1132
||||||||| |||| |||| | |||||||||| |||||||||| |||| | ||||||||
Sbjct 1121 GCAGAGCTAGAGCTTATAACTATATGTGATGTAGAAGATGTTTCGAAAGCTTCATGGAAC 1180
Query 1133 GTTCCTCTTAAAGATTGTGTGCAAATCAGTGGACA-A-ACAGAAGAGAGACCCTCTTCTT 1190
|||||||||| ||| |||||| | || |||| | | | ||| |||| | |||||||||||
Sbjct 1181 GTTCCTCTTAGAGACTGTGTGGACATTAGTGAAAACATACAAAAGA-A-ACCCTCTTCTT 1238
Query 1191 TAGCTGAACGTCTTTC-TGCATATCCAGCAACTCTAAGA-AAAATAGGGATCAGTGAAGA 1248
| ||| ||||| || | |||||||| ||| ||||| | |||| ||| ||| | |||||
Sbjct 1239 CAACTGGCCGTCTATCGT-CATATCCAACAAGTCTAACAGAAAA-AGGCATCGGCGAAGA 1296
Query 1249 TGAATATACATCAGATACAGTGTTCTGGAGAGAACAAGTTAATCATTACTGGCG-GTTAA 1307
||||| | ||| || ||| ||||||||||||||||| ||||| |||||| | |||||
Sbjct 1297 TGAATTTGCATTGGACACAAACTTCTGGAGAGAACAAGTGAATCAGTACTGG-GAGTTAA 1355
Query 1308 TGAATGTCAATGAGACTGAAGTACGGAATGTGATGGACATGAACGCATTCATTGGTGGTT 1367
||||||| ||| |||||||||| || ||||||||||||| || ||| ||||||||| |
Sbjct 1356 TGAATGTTAATAAGACTGAAGTGCGAAATGTGATGGACACAAATGCAGCCATTGGTGGAT 1415
Query 1368 TTGCTGCAGCTATGAATTCGTATCCTGTTTGGGTAATGAACATAGTACCTGCAACCATGA 1427
|||| ||||| ||||| || ||||| |||||||| |||||| | |||||||| || ||||
Sbjct 1416 TTGCCGCAGCCATGAACTCATATCCCGTTTGGGTCATGAACGTTGTACCTGCTACGATGA 1475
Query 1428 ATGATACCTTGTCTGGAATTTTCGAGAGAGGCTTAAATGGCGCTT-TCCATGATTGGTGT 1486
| |||||||| || ||||||| | |||| ||||||| ||| |||| || |||||||||||
Sbjct 1476 ACGATACCTTATCCGGAATTTACCAGAGGGGCTTAACTGGTGCTTATC-ATGATTGGTGT 1534
Query 1487 GAGGCGTTCTCAACATATCCACGCACGTATGATTTGGTGCATTCCGATCATGTCTTCTCT 1546
||| | |||||||| ||||| || || || ||| || | || | || ||| | ||| ||
Sbjct 1535 GAGCCATTCTCAACGTATCCCCGTACTTACGATCTGCTACACGCGGACCATCTTTTCGCT 1594
Query 1547 CACTACAACAAAAGCTACGGAGATGGTTGTTTGCTAGAGGATATCATGCTTGAGATGGAC 1606
||||| || || | |||| || |||||||| |||||||| || |||||||||||||||
Sbjct 1595 CACTATAA-AATC-C-ACGGTGAAGGTTGTTTACTAGAGGACATGATGCTTGAGATGGAC 1651
Query 1607 CGCAT-AGTTCGCCCTCAGGGGTTTGTCATAATAAGGGACGAGGAAT-ATATCATCTCAA 1664
||||| | | ||||||||||| || |||| || || |||||||||| | |||||||| |
Sbjct 1652 CGCATTA-TACGCCCTCAGGGATTCATCATTATCAGAGACGAGGAATCA-ATCATCTCGA 1709
Query 1665 G-GATCCGAGGCTTGGCTCCAAAATTC-TTGTGGGAAGTGGAAACTCATGAGCTTGAAAA 1722
| | |||||| ||||||||| || ||| || |||||||| ||||| ||||||||| || |
Sbjct 1710 GAG-TCCGAGACTTGGCTCCTAAGTTCCTT-TGGGAAGTTGAAACACATGAGCTTCAAGA 1767
Query 1723 CAAAGACAAGAAGATAACAGAGTCTGTTTTGTTTTGCAGAAAGAGATTCTGGGCAATCAT 1782
|||| ||||||||| |||| ||||| | ||||||||||||| ||| ||||||||| |
Sbjct 1768 CAAATACAAGAAGA---CAGAAACTGTTCTATTTTGCAGAAAGATATTTTGGGCAATCGT 1824
Query 1783 TTGAAA 1788
||||||
Sbjct 1825 TTGAAA 1830
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 73590353340
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5