BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT3G56440.1
Length=1656
Score E
Sequences producing significant alignments: (Bits) Value
Ahg486086 jgi|Araly1|486086|fgenesh2_kg.5__2078__AT3G56440.1 2591 0.0
Ahg321769 jgi|Araly1|321769|fgenesh1_pm.C_scaffold_4001826 1059 0.0
Ahg901662 jgi|Araly1|901662|Al_scaffold_0004_2312 665 0.0
Ahg486087 jgi|Araly1|486087|fgenesh2_kg.5__2079__AT3G56460.1 80.5 4e-14
> Ahg486086 jgi|Araly1|486086|fgenesh2_kg.5__2078__AT3G56440.1
Length=1600
Score = 2591 bits (1403), Expect = 0.0
Identities = 1547/1614 (96%), Gaps = 19/1614 (1%)
Strand=Plus/Plus
Query 10 AATGACGACAGTTGACTAACGCAAACAACCGAATAAAGAAAGAACAAAATCGGAAATTTT 69
|||||||||||||||||||| ||||||| |||||||| |||||| |||||||||||||||
Sbjct 1 AATGACGACAGTTGACTAACCCAAACAAACGAATAAAAAAAGAAAAAAATCGGAAATTTT 60
Query 70 CGACGAAGATAAAATCATACAAGTTAGTA-TACAGAGACG-A--TTCTCATAGCCAAGTC 125
||||||| || || |||||||||||| |||||||||| | ||||||||||||||||
Sbjct 61 CGACGAA-AT--CAT--TACAAGTTAGTATTACAGAGACGAACCTTCTCATAGCCAAGTC 115
Query 126 ATTGATTTGAGAAGATAACAAGATTATTATAGCATCAGGATCGAGATTGTTCGTCGTCCA 185
|||| || | | | | ||| ||||||||||||||||||||||||||| |||||||
Sbjct 116 ATTG--TT--G-ATTTGAGAAG---ATTATAGCATCAGGATCGAGATTGTTCTTCGTCCA 167
Query 186 TTGAAGCAGCATTTTCCTTCTTCAAAGATCGCTTTTGGTTCTTCTCGCTGTTTTCGTTTT 245
|||||||||||||||||||||||||||||||| |||| ||||||||||||||||||||||
Sbjct 168 TTGAAGCAGCATTTTCCTTCTTCAAAGATCGCGTTTGCTTCTTCTCGCTGTTTTCGTTTT 227
Query 246 CT-AATCGCTTGCAACAATTTCAACGTTTCACATATTAAGGAGAAATGGATCCTCGGAGA 304
|| ||||||||||||||||| ||||| ||||||||||||||||||||||| ||||| |||
Sbjct 228 CTGAATCGCTTGCAACAATTGCAACGCTTCACATATTAAGGAGAAATGGACCCTCGAAGA 287
Query 305 AACTTCCAACCAGGTGGCTATGACTCTCGGAATACCTTCACTTCAGGATCTTTTGGGCCT 364
|||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 288 AACTTCCAACCAGGTGGCTATGACTCCCGGAATACCTTCACTTCAGGATCTTTTGGGCCT 347
Query 365 CCAGATTTTGGTGAAAGTGATGAAGCAGAGTTGGTTTCTGTTTCTTGGAATCAGGATTAC 424
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 348 CCAGATTTTGGTGAAAGTGATGAAGCAGAGTTGGTTTCTGTTTCTTGGAATCAGGATTAC 407
Query 425 AGTTGTTTTGCTGCTGGCACTAGCCATGGTTTTCGTATATATAACTGTGAACCGTTCAAG 484
|| |||||||||||||| || |||||||||||||||||||| ||||||||||||||||||
Sbjct 408 AGCTGTTTTGCTGCTGGTACGAGCCATGGTTTTCGTATATACAACTGTGAACCGTTCAAG 467
Query 485 GAAACTTTTAGGCGAGAGCTGAAGGATGGAGGGTTTAAAATTGTAGAGATGCTTTTCCGG 544
|||||||| || ||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 468 GAAACTTTCAGACGAGAGCTGAAGGGTGGAGGGTTTAAAATTGTAGAGATGCTTTTCCGG 527
Query 545 AGTAATATCTTGGCGCTTGTTGGTGGTGGACCTAACTCCCAGTACCCTTCAAATAAAGTA 604
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 528 AGTAATATCTTGGCGCTTGTTGGTGGTGGACCTAACTCTCAGTACCCTTCAAATAAAGTA 587
Query 605 TTGATTTGGGATGATCATCAGGGGCGCTGCATCAGTGAGTTTACTTTTCGGTCGGAAATT 664
||||||||||||||||||||| | ||||||||||||||||||||||||||||||||||||
Sbjct 588 TTGATTTGGGATGATCATCAGAGCCGCTGCATCAGTGAGTTTACTTTTCGGTCGGAAATT 647
Query 665 CGTGCAGTGAAGTTAAGAAGAGATCGAATTGTTGTTGTTCTTGAGCACAAGATATATGTC 724
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct 648 CGTGCAGTGAAGTTAAGAAGAGATCGAATTGTCGTTGTTCTTGAGCACAAGATATATGTC 707
Query 725 TACAATTTTATGGATCTAAGACTTCTTCATCAGATTGAAAATATGGCAAATCCAAGGGGA 784
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 708 TACAATTTTATGGATCTAAGACTTCTTCATCAGATTGAAAATCTGGCAAATCCAAGGGGA 767
Query 785 CTATGTTGCCTCTCTCATCACATGAATACATCTGTCTTGGCTTGTCCAGGTATTCGTCGA 844
|||||||||||||||||||| ||||||||||||||| |||| ||||||||||||||||||
Sbjct 768 CTATGTTGCCTCTCTCATCATATGAATACATCTGTCCTGGCCTGTCCAGGTATTCGTCGA 827
Query 845 GGAGAGGTCCGAGTTGAACATTTTGGGCTTAACATGGTTCAAATCATTAATGCTCATGAT 904
|||||||||||||||||||||||||| |||||||||||||||||||| ||||||||||||
Sbjct 828 GGAGAGGTCCGAGTTGAACATTTTGGTCTTAACATGGTTCAAATCATCAATGCTCATGAT 887
Query 905 TCTAATATCGCGTGCATGACCTTGACATTGGATGGCTTGCTCCTTGCAACTGCCAGTACA 964
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 888 TCTAATCTCGCGTGCATGACCTTGACATTGGATGGCTTGCTCCTTGCAACTGCCAGTACA 947
Query 965 AAGGGAACTCTGATTAGAATTTTCAACACGATGGACGGAACTCGTCTGCAAGAGGTCCGA 1024
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 948 AAGGGAACTCTGATTAGAATTTTCAACACGATGGACGGAACTCGTCTGCAAGAGGTCCGA 1007
Query 1025 AGAGGCGTGGACAGAGCAGATATCTATAGCATTGCTCTATCACCAAACGTGCAGTGGCTT 1084
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1008 AGAGGCGTGGACAGAGCAGATATCTATAGCATTGCTCTATCACCAAACGTGCAGTGGCTT 1067
Query 1085 GCAGTATCAAGCGACAAGGGGACTGTTCACATTTTCTCTCTCAGAGTGAGAGTTATAGGG 1144
|||||||||||||||||||||||||||||||||||||||||||||||||| ||||| |||
Sbjct 1068 GCAGTATCAAGCGACAAGGGGACTGTTCACATTTTCTCTCTCAGAGTGAGGGTTATTGGG 1127
Query 1145 GAGGACGCATACTCCACTGAACATGAGACTTCTTCAAATTCCCTGCAGCCTCTTGTTTCT 1204
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1128 GAGGACGCATACTCCACTGAACATGAGACTTCTTCAAATTCCCTGCAGCCTCTTGTTTCT 1187
Query 1205 CCAGCCAGTGGTGCCAACCCTGGTTCGTCATTGTCTTTTCTGAGAGGTGTTCTACCAAAA 1264
|||||||||||||||||||| |||||||||||||||||||||||||| ||||||||||||
Sbjct 1188 CCAGCCAGTGGTGCCAACCCCGGTTCGTCATTGTCTTTTCTGAGAGGAGTTCTACCAAAA 1247
Query 1265 TATTTCAGCTCGGAATGGTCATTTTCTCAGTTCCATGTACCTGAAGTCACCCAATACTTT 1324
|||||||||||||||||||||||||| |||||||||||||| ||||||||||||||||||
Sbjct 1248 TATTTCAGCTCGGAATGGTCATTTTCCCAGTTCCATGTACCAGAAGTCACCCAATACTTT 1307
Query 1325 GCAGCATTTGGTGCTCAGAACACAATTGCAATTATTGGCTTGGACGGAAGTTTCTACAGG 1384
||||||||||| ||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 1308 GCAGCATTTGGCGCTCAGAACACAATTGCGATTATTGGCTTGGACGGAAGTTTCTACAGG 1367
Query 1385 TGCAATTTCGATCCAGTGAACGGAGGAGAGATGACTCAGCTTGAACATTTCCATTTTCTG 1444
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1368 TGCAATTTCGATCCAGTGAACGGAGGAGAGATGACTCAGCTTGAACATTTCCATTTTCTG 1427
Query 1445 AAGCAAGATAGCCCGAGATAGTCAGACCACGTGCTTGGTTTGAGTTTTATGTACATAACA 1504
|||| ||||||||| ||||||| ||| ||||||||||||||||||||||| |||||||||
Sbjct 1428 AAGCCAGATAGCCCAAGATAGTTAGAACACGTGCTTGGTTTGAGTTTTATATACATAACA 1487
Query 1505 ATTGGCTCTGTATATAGATTTGGTGAAACCGCATACGATTTTATTCTTCCAAGCTTTACT 1564
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1488 ATTGGCTCTGTATATAGATTTGGTGAAACCGCATACGATTTTATTCTTCCAAGCTTTACT 1547
Query 1565 TGATTGAAGGAAAATGTATTCTTGATTGGAACAATGTATATACAAAAAGGAttt 1618
||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1548 TGATTGAAGGAAA-TGTATTCTTGATTGGAACAATGTATATACAAAAAGGATTT 1600
> Ahg321769 jgi|Araly1|321769|fgenesh1_pm.C_scaffold_4001826
Length=1215
Score = 1059 bits (573), Expect = 0.0
Identities = 924/1090 (85%), Gaps = 38/1090 (3%)
Strand=Plus/Plus
Query 386 GAAGCAGAGTTGGTTTCTGTTTCTTGGAATCAGGATTAC-AGTTGTTTTGCTGCTGGCAC 444
|||||||||||||||||||| | |||||||||||||| | ||||||||||| ||||| ||
Sbjct 115 GAAGCAGAGTTGGTTTCTGTGTGTTGGAATCAGGATT-CAAGTTGTTTTGCAGCTGGAAC 173
Query 445 TAGCCATGGTTTTCGTATATATAACTGTGAACCGTTCAAGGAAACTTTTAGGCGAGAGCT 504
|| |||||||||||||||||||||||| |||| |||||||||||||| ||||| |||||
Sbjct 174 GAGTCATGGTTTTCGTATATATAACTGTCAACCTTTCAAGGAAACTTTCAGGCGTGAGCT 233
Query 505 GAAGGATGGAGGGTTTAAAATTGTAGAGATGCTTTTCCGGAGTAATATCTTGGCGCTTGT 564
||| | || || |||||||||||||||||||||||||| || ||||| ||||| |||||
Sbjct 234 AAAGAACGGCGGTTTTAAAATTGTAGAGATGCTTTTCCGAAGCAATATATTGGCACTTGT 293
Query 565 TGGTGGTGGACCTAACTCCCAGTACCCTTCAAATAAAGTATTGATTTGGGATGATCATCA 624
|||||||||||||||||| |||||||||||||| |||||| | |||||||||||||||||
Sbjct 294 TGGTGGTGGACCTAACTCTCAGTACCCTTCAAACAAAGTACTAATTTGGGATGATCATCA 353
Query 625 GGGGCGCTGCATCAGTGAGTTTACTTTTCGGTCGGAAATTCGTGCAGTGAAGTTAAGAAG 684
| | || ||||||||||| ||| | ||| |||| || |||||||| ||||| ||||||||
Sbjct 354 GAGCCGTTGCATCAGTGAATTTGCATTTAGGTCTGAGATTCGTGCTGTGAAATTAAGAAG 413
Query 685 AGATCGAATTGTTGTTGTTCTTGAGCACAAGATATATGTCTACAATTTTATGGATCTAAG 744
||||| ||||||||||||||||| || ||||| ||||| |||||||||||||| || ||
Sbjct 414 GGATCGGATTGTTGTTGTTCTTGAACATAAGATTTATGTTTACAATTTTATGGACCTTAG 473
Query 745 ACTTCTTCATCAGATTGAAA-ATATGGCAAATCCAAGGGGACTATGTTGCCTCTCTCATC 803
|||||||||||||||||||| | || ||||| || ||| |||||||||| |||||||
Sbjct 474 ACTTCTTCATCAGATTGAAACCCA-AGCTAATCCTAGAGGATTATGTTGCCTTTCTCATC 532
Query 804 ACAT-GAATACATCTGT-CTTGGCTTGTCCAGGTATTCGTCGAGGAGAGGTCCGAGTTGA 861
| | ||||| ||||| | | ||||| || ||| ||| |||||||||| | ||||||||
Sbjct 533 A-TTCTAATACGTCTGTGC-TCGCTTGCCCCGGTCTTCATCGAGGAGAGATTCGAGTTGA 590
Query 862 ACATTTTGGGCTTAACATGGTTCAAATCATTAATGCTCATGATTCTAATATCGCGTGCAT 921
|||||| |||||||||||||| |||||||| |||||||||||||||| ||| ||||| ||
Sbjct 591 ACATTTCGGGCTTAACATGGTACAAATCATAAATGCTCATGATTCTAGTATTGCGTGTAT 650
Query 922 GACCTTGACATTGGATGGCTTGCTCCTTGCAACTGCCAGTACAAAGGGAACTCTGATTAG 981
||| |||||||||||||| ||| | || |||||||| |||||||||||||||||||||||
Sbjct 651 GACTTTGACATTGGATGGTTTGTTGCTCGCAACTGCTAGTACAAAGGGAACTCTGATTAG 710
Query 982 AATTTTCAACACGATGGACGGAACTCGTCTGCAAGAGGTCCGAAGAGGCGTGGACAGAGC 1041
||| |||||||| ||||||||||||||| |||||||||| |||||||| |||||||||||
Sbjct 711 AATCTTCAACACTATGGACGGAACTCGTTTGCAAGAGGTACGAAGAGGAGTGGACAGAGC 770
Query 1042 AGATATCTATAGCATTGCTCTATCACCAAACGTGCAGTGGCTTGCAGTATCAAGCGACAA 1101
|||||| ||||||||||| || |||||||||||||||||||| || ||||||||||||||
Sbjct 771 AGATATTTATAGCATTGCACTTTCACCAAACGTGCAGTGGCTGGCTGTATCAAGCGACAA 830
Query 1102 GGGGACTGTTCACATTTTCTCTCTCAGAGTGAGAGTTA-TAGGGGAGGACGCATACTCCA 1160
|||||||||||||||||||||||| ||||| || || | | |||||||| | || ||||
Sbjct 831 GGGGACTGTTCACATTTTCTCTCTTAGAGTTAGGGT-AGTTGGGGAGGATTCTTATTCCA 889
Query 1161 CTGAACATG-AGACT-TCTT--CA-AA-----TT---C--C--CT--GCA-GCCTCTTGT 1200
||||| ||| || || || | || || || | | || ||| | | |||||
Sbjct 890 CTGAAAATGCAG-CTCTCCTGACACAACAAACTTATTCTACTTCTCTGCAAGGC-CTTGT 947
Query 1201 TTCTCCAGCCAGTGGTGCCAA-CCCTGGTTCGTCATTGTCTTTTCTGAGAGGTGTTCTAC 1259
||||||| ||| |||| |||| ||| |||||||||||||| ||| |||||||||||||||
Sbjct 948 TTCTCCAACCACTGGTACCAATCCC-GGTTCGTCATTGTCGTTTATGAGAGGTGTTCTAC 1006
Query 1260 CAAAATATTTCAGCTCGGAATGGTCATTTTCTCAGTTCCATGTACCTGAAGTCACCCAAT 1319
|||| |||||||||||||||||||||| | | ||||| |||||| | |||||||| ||||
Sbjct 1007 CAAAGTATTTCAGCTCGGAATGGTCATATGCACAGTTTCATGTATCAGAAGTCACACAAT 1066
Query 1320 ACTTTGCAGCATTTGGT-GCTCAGAACACAATTGCAATTATTGGCTTGGACGGAAGTTTC 1378
||| ||||||||||| | | | ||||| |||| ||||| ||| |||| ||||||||
Sbjct 1067 TCTTCGCAGCATTTGGCAG-TAACAACACGGTTGCTATTATCGGCATGGATGGAAGTTTT 1125
Query 1379 TACAGGTGCAA-TTTCGATCCAGTGAACGGAGGAGAGATGACTCAGCTTGAACATTTCCA 1437
||||| |||| ||| ||||| |||||||||||||||||| || || ||| |||||||
Sbjct 1126 TACAGATGCAGCTTT-GATCCCGTGAACGGAGGAGAGATGGGGCAACTGGAATATTTCCA 1184
Query 1438 TTTTCTGAAG 1447
|||||||||
Sbjct 1185 CTTTCTGAAG 1194
> Ahg901662 jgi|Araly1|901662|Al_scaffold_0004_2312
Length=1170
Score = 665 bits (360), Expect = 0.0
Identities = 630/756 (83%), Gaps = 36/756 (5%)
Strand=Plus/Plus
Query 719 TATGTCTACAATTTTATGGATCTAAGACTTCTTCATCAGATTGAAA-ATATGGCAAATCC 777
||||| |||||||||||||| || |||||||||||||||||||||| | || |||||
Sbjct 403 TATGTTTACAATTTTATGGACCTTAGACTTCTTCATCAGATTGAAACCCA-AGCTAATCC 461
Query 778 AAGGGGACTATGTTGCCTCTCTCATCACAT-GAATACATCTGT-CTTGGCTTGTCCAGGT 835
|| ||| |||||||||| |||||||| | ||||| ||||| | | ||||| || |||
Sbjct 462 TAGAGGATTATGTTGCCTTTCTCATCA-TTCTAATACGTCTGTGC-TCGCTTGCCCCGGT 519
Query 836 ATTCGTCGAGGAGAGGTCCGAGTTGAACATTTTGGGCTTAACATGGTTCAAATCATTAAT 895
||| |||||||||| | |||||||||||||| |||||||||||||| |||||||| |||
Sbjct 520 CTTCATCGAGGAGAGATTCGAGTTGAACATTTCGGGCTTAACATGGTACAAATCATAAAT 579
Query 896 GCTCATGATTCTAATATCGCGTGCATGACCTTGACATTGGATGGCTTGCTCCTTGCAACT 955
||||||||||||| ||| ||||| ||||| |||||||||||||| ||| | || ||||||
Sbjct 580 GCTCATGATTCTAGTATTGCGTGTATGACTTTGACATTGGATGGTTTGTTGCTCGCAACT 639
Query 956 GCCAGTACAAAGGGAACTCTGATTAGAATTTTCAACACGATGGACGGAACTCGTCTGCAA 1015
|| |||||||||||||||||||||||||| |||||||| ||||||||||||||| |||||
Sbjct 640 GCTAGTACAAAGGGAACTCTGATTAGAATCTTCAACACTATGGACGGAACTCGTTTGCAA 699
Query 1016 GAGGTCCGAAGAGGCGTGGACAGAGCAGATATCTATAGCATTGCTCTATCACCAAACGTG 1075
||||| |||||||| ||||||||||||||||| ||||||||||| || ||||||||||||
Sbjct 700 GAGGTACGAAGAGGAGTGGACAGAGCAGATATTTATAGCATTGCACTTTCACCAAACGTG 759
Query 1076 CAGTGGCTTGCAGTATCAAGCGACAAGGGGACTGTTCACATTTTCTCTCTCAGAGTGAGA 1135
|||||||| || |||||||||||||||||||||||||||||||||||||| ||||| ||
Sbjct 760 CAGTGGCTGGCTGTATCAAGCGACAAGGGGACTGTTCACATTTTCTCTCTTAGAGTTAG- 818
Query 1136 GTTA-TAGGGGAGGACGCATACTCCACTGAACATG-AGACT-TCTT--CA-AA-----TT 1184
| || | |||||||| | || ||||||||| ||| || || || | || || ||
Sbjct 819 GCTAGTTGGGGAGGATTCTTATTCCACTGAAAATGCAG-CTCTCCTGACACAACAAACTT 877
Query 1185 ---C--C--CT--GCA-GCCTCTTGTTTCTCCAGCCAGTGGTGCCAA-CCCTGGTTCGTC 1233
| | || ||| | | ||||||||||| ||| |||| |||| ||| ||||||||
Sbjct 878 ATTCTACTTCTCTGCAAGGCA-TTGTTTCTCCAACCACTGGTACCAATCCC-GGTTCGTC 935
Query 1234 ATTGTCTTTTCTGAGAGGTGTTCTACCAAAATATTTCAGCTCGGAATGGTCATTTTCTCA 1293
|||||| ||| ||||||||||||||||||| |||||||||||||||||||||| | | ||
Sbjct 936 ATTGTCGTTTATGAGAGGTGTTCTACCAAAGTATTTCAGCTCGGAATGGTCATATGCACA 995
Query 1294 GTTCCATGTACCTGAAGTCACCCAATACTTTGCAGCATTTGGT-GCTCAGAACACAATTG 1352
||| |||||| | |||||||| |||| ||| ||||||||||| | | | ||||| |||
Sbjct 996 GTTTCATGTATCAGAAGTCACACAATTCTTCGCAGCATTTGGCAG-TAACAACACGGTTG 1054
Query 1353 CAATTATTGGCTTGGACGGAAGTTTCTACAGGTGCAA-TTTCGATCCAGTGAACGGAGGA 1411
| ||||| ||| |||| |||||||| ||||| |||| ||| ||||| ||||||||||||
Sbjct 1055 CTATTATCGGCATGGATGGAAGTTTTTACAGATGCAGCTTT-GATCCCGTGAACGGAGGA 1113
Query 1412 GAGATGACTCAGCTTGAACATTTCCATTTTCTGAAG 1447
|||||| || || ||| ||||||| |||||||||
Sbjct 1114 GAGATGGGGCAACTGGAATATTTCCACTTTCTGAAG 1149
> Ahg486087 jgi|Araly1|486087|fgenesh2_kg.5__2079__AT3G56460.1
Length=1294
Score = 80.5 bits (43), Expect = 4e-14
Identities = 43/43 (100%), Gaps = 0/43 (0%)
Strand=Plus/Minus
Query 1576 AAATGTATTCTTGATTGGAACAATGTATATACAAAAAGGAttt 1618
|||||||||||||||||||||||||||||||||||||||||||
Sbjct 1294 AAATGTATTCTTGATTGGAACAATGTATATACAAAAAGGATTT 1252
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 62087099350
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5