BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT3G59052.1
Length=2039
Score E
Sequences producing significant alignments: (Bits) Value
Ahg486367 jgi|Araly1|486367|fgenesh2_kg.5__2359__AT3G59052.1 2953 0.0
Ahg483403 jgi|Araly1|483403|fgenesh2_kg.4__2463__AT2G43018.1 1275 0.0
> Ahg486367 jgi|Araly1|486367|fgenesh2_kg.5__2359__AT3G59052.1
Length=1997
Score = 2953 bits (1599), Expect = 0.0
Identities = 1871/1998 (94%), Gaps = 36/1998 (2%)
Strand=Plus/Plus
Query 47 CTCATTG-TTTTGCTTCAAATTACGCATTCGAATCTGATCTTCGTTGATCTCTGTGGAAG 105
||| ||| |||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 29 CTCTTTGATTTTGCTTCAGATTACGCATTCGAATCTGATCTTCGTTGATCTCTGTGGAAG 88
Query 106 AAACAGATAAGAGAAAATCGCATGAAtttttttAGAAAACTAGGTTTTAATCCTTCGATT 165
|||| |||||||||||||||||||||||||||| ||||||||||||||||| | ||||||
Sbjct 89 AAACTGATAAGAGAAAATCGCATGAATTTTTTTGGAAAACTAGGTTTTAATTCATCGATT 148
Query 166 GCAAGCTACTTCACTAATACGAGTCTTCTGATCTCAATTCTTAGTTTTTCTCCTCAATTG 225
|||||||||||||||||||| |||||||||| |||||||||||||||||||||||||||
Sbjct 149 TCAAGCTACTTCACTAATACGTGTCTTCTGATTTCAATTCTTAGTTTTTCTCCTCAATTG 208
Query 226 CCGATTTCTCTCTTTCTTTTCGAGCAGAAATCGTTAGATCCTTTATCGCTG--T-GTAAT 282
|||||||| ||||||||||| |||||||||||||||||||||||||||||| | || |
Sbjct 209 CCGATTTCGCTCTTTCTTTTTGAGCAGAAATCGTTAGATCCTTTATCGCTGAATAATACT 268
Query 283 AGTAGTACTAATTTTAATAATCTTGAGAGTAATTACTCCGTTCATTGTTGAATCATGGAG 342
|||| ||||||||||||||||| | ||||||||| || | |||||||||||||||||||
Sbjct 269 AGTAATACTAATTTTAATAATCCCGTGAGTAATTAGTCTGATCATTGTTGAATCATGGAG 328
Query 343 TCCGGAGGCAAAACTAATCGTCAGCTGCGTAAAGCTATTTGCGTCTCAACAGACGAGAAG 402
||||||||||||||||||||||||||||||||||||||||| |||||| | |||||||||
Sbjct 329 TCCGGAGGCAAAACTAATCGTCAGCTGCGTAAAGCTATTTGTGTCTCAGCCGACGAGAAG 388
Query 403 ATGAAGAAGAAGAGATCACCTTCAGTGATAGTGATTGGAGGTGGTATGGCTGGAATTTCA 462
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 389 ATGAAGAAGAAGAGATCACCTTCAGTGATAGTGATTGGAGGTGGTATGGCTGGAATTTCA 448
Query 463 GCCGCTCGCACTCTTCAGGATGCTTCCTTTCAGGTTGTTGTATTGGAGTCTCGTGACAGA 522
|| |||||||||||||| |||||||||||||||||||| || |||||||||||||| |||
Sbjct 449 GCTGCTCGCACTCTTCAAGATGCTTCCTTTCAGGTTGTGGTGTTGGAGTCTCGTGATAGA 508
Query 523 ATTGGTGGACGAGTTCACACTGATTACTCATTTGGTTTTCCAGTTGATCTCGGTGCATCT 582
|||||||||||||||||||||||||||||||| ||||||||||||||||| |||||||||
Sbjct 509 ATTGGTGGACGAGTTCACACTGATTACTCATTCGGTTTTCCAGTTGATCTGGGTGCATCT 568
Query 583 TGGTTGCATGGTGTCTGTAAAGAGAATCCTTTGGCTGCAGTAATTGGTAGATTAGGATTA 642
|||||||||||||||||||||||||||||| |||||||||||||||| ||||||||||||
Sbjct 569 TGGTTGCATGGTGTCTGTAAAGAGAATCCTCTGGCTGCAGTAATTGGGAGATTAGGATTA 628
Query 643 CCTCTTTATCGTACTAGCGGTGACAATTCGGTTTTGTATGATCACGATCTTGAGAGTTAT 702
|| |||||||||||||| |||||||||||||||||||||||||||||||||||||| |||
Sbjct 629 CCCCTTTATCGTACTAGTGGTGACAATTCGGTTTTGTATGATCACGATCTTGAGAGCTAT 688
Query 703 GCTCTGTTCGACAAGGCTGGTAACCAAGTTTCTCAAGAGTTGGTGACAAAAGTTGGCGAA 762
||||| |||||||||||||| ||||||||| ||||||||||||| |||||||||||||||
Sbjct 689 GCTCTATTCGACAAGGCTGGCAACCAAGTTCCTCAAGAGTTGGTAACAAAAGTTGGCGAA 748
Query 763 AATTTTGAGCACATTCTGGAAGAGATCTGTAAAGTCAGGGATGAGCAGGACGAAGACATG 822
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 749 AATTTTGAGCACATTCTGGAAGAGATCTCTAAAGTCAGGGATGAGCAGGACGAAGACATG 808
Query 823 TCAATAGCTCAAGCCTTTTCTATTGTATTTAAGAGGAACCCTGAATTGAGGTTGGAGGGA 882
||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 809 TCAATAGCTCAAGCCTTTTCTATTGTATTCAAGAGGAACCCTGAATTGAGGTTGGAGGGG 868
Query 883 CTTGCCCATAATGTGCTTCAGTGGTACTTGTGCCGCATGGAAGGATGGTTTGCTGCTGAT 942
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 869 CTTGCTCATAATGTGCTTCAGTGGTACTTGTGCCGCATGGAAGGATGGTTTGCTGCTGAT 928
Query 943 GCTGAAACCATCTCGGCAAAGTGTTGGGATCAGGAAGAACTGTTGCCCGGTGGACATGGT 1002
||||||||||||||||||||||||||||| ||||||||||| ||||| ||||||||||||
Sbjct 929 GCTGAAACCATCTCGGCAAAGTGTTGGGACCAGGAAGAACTATTGCCTGGTGGACATGGT 988
Query 1003 CTTATGGTTAGAGGGTATCGCCCTGTAATCAACACTCTCTCGAAAGGTCTTGATATACGA 1062
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 989 CTTATGGTTAGAGGGTATCGCCCTGTAATCAACACTCTCTCGAAAGGTCTTGATATACGA 1048
Query 1063 CTAAGTCACAGAATTACCAAGATT-TCAAGGCGCTACAGTGGTGTGAAGGTGACTACAGA 1121
||||| ||||| ||||||||||| | ||||||||||||||| |||||||||||||||||
Sbjct 1049 CTAAGCCACAGGGTTACCAAGATTGT-AAGGCGCTACAGTGGAGTGAAGGTGACTACAGA 1107
Query 1122 GAAAGGAGACACGTTTGTTGCGGATGCTGCAGTCATTGCTCTTCCTCTAGGAG-TCTTAA 1180
|||||||||||||||||||||||||||||||||||||||||||||||| |||| || |||
Sbjct 1108 GAAAGGAGACACGTTTGTTGCGGATGCTGCAGTCATTGCTCTTCCTCTTGGAGTTC-TAA 1166
Query 1181 AGTCAGGAATGATAACATTCGAACCAAAGCTTCCACAGTGGAAACAAGAAGCTATCAACG 1240
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 1167 AGTCAGGAATGATAACATTCGAACCAAAGCTTCCACAGTGGAAACAGGAAGCTATCAACG 1226
Query 1241 ATCTCGGTGTTGGAATCGAGAACAAGATCATATTGAATTTTGACAACGTCTTTTGGCCAA 1300
|||||||||| ||||||||||||||||||||| || | |||||||| |||||||||||||
Sbjct 1227 ATCTCGGTGTAGGAATCGAGAACAAGATCATACTGCACTTTGACAATGTCTTTTGGCCAA 1286
Query 1301 ACGTAGAGTTTTTAGGAGTCGTTGCAGAAACTTCCTACGGATGCAGTTATTTCTTGAACC 1360
||||||||||| ||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1287 ACGTAGAGTTTCTAGGAGTGGTTGCAGAAACTTCCTACGGATGCAGTTATTTCTTGAACC 1346
Query 1361 TGCATAAAGCCACAAGCCATCCTGTCCTTGTTTACATGCCGGCTGGTCAGCTCGCTAGAG 1420
|||||||||||||||||||||| || ||||||||||||||||||||||||||||||||||
Sbjct 1347 TGCATAAAGCCACAAGCCATCCCGTTCTTGTTTACATGCCGGCTGGTCAGCTCGCTAGAG 1406
Query 1421 ACATCGAGAAAAAGTCCGATGAAGCAGCTGCAAATTTTGCCTTTTCGCAACTACAGAAAA 1480
|||| ||||||||||| |||||| |||||||||||||||||||||||||||| |||||||
Sbjct 1407 ACATAGAGAAAAAGTCTGATGAATCAGCTGCAAATTTTGCCTTTTCGCAACTCCAGAAAA 1466
Query 1481 TTCTTCCAGATGCCTCTTCACCGATAAATTATTTGGTATCAAGGTGGGGATCAGACATCA 1540
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1467 TTCTTCCAGATGCATCTTCACCGATAAATTATTTGGTATCAAGGTGGGGATCAGACATCA 1526
Query 1541 ATTCATTGGGATCATATAGCTATGACATTGTGAACAAACCTCACGACCTCTATGAGAGAC 1600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1527 ATTCATTGGGATCATATAGCTATGACATTGTGAACAAACCTCACGACCTCTATGAGAGAC 1586
Query 1601 TGAGAGTGCCATTGGATAATCTATTCTTTGCGGGGGAAGCCACTAGCTCAAGCTACCCGG 1660
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 1587 TGAGAGTGCCATTGGATAATCTATTCTTTGCGGGGGAAGCCACTAGCTCGAGCTACCCGG 1646
Query 1661 GATCAGTGCACGGTGCTTATTCAACCGGAGTGCTGGCAGCTGAAGACTGCAGGATGCGTG 1720
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1647 GGTCAGTGCACGGTGCTTATTCAACCGGAGTGCTGGCAGCTGAAGACTGCAGGATGCGTG 1706
Query 1721 TGCTTGAGCGCTATGGAGAGCTCGAACATGAGATGGAAGAGGAAGCTCCTGCTTCTGTCC 1780
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
Sbjct 1707 TGCTTGAGCGCTATGGAGAGCTTGAACATGAGATGGAAGAGGAAGCTCCTGCTTCTGTCC 1766
Query 1781 CTCTTCTGATCTCCCGTATGTAATTTAAACCTCAGGTGTTTTATATCACCTCCATATGGG 1840
||||| |||||||||||||||| ||| |||||||||| | ||| || | || ||
Sbjct 1767 CTCTTTTGATCTCCCGTATGTAGTTTGAACCTCAGGT-TGTTAGATT---TG-ATTTG-- 1819
Query 1841 AAC-ATTGTTAGATTTGATTTTATGAGATTTCACTAGTTGGAACTCTTGTGCCTATTCAA 1899
| | ||| | | | ||||| || | | |||||||||||||||| | || | |
Sbjct 1820 A-CTATT-T-A--T--GATTTCAT-A--T--CACTAGTTGGAACTCT----CA-ATGCCA 1862
Query 1900 TACCACTCTTTTTTCCTTCAAATCTGATTCAGATCTTTAGTCGAAGTCAAATGTGCAATG 1959
| ||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1863 --C-ACTCTGATTTCCTTCAAATCTGATTCAGATCTTTAGTCGAAGTCAAATGTGCAATG 1919
Query 1960 TGGAGTATGTAATCCATCTGATATAAGAACTATGTTTAAGTATCAGTGCCAAATGCAAAC 2019
|||||||||||||||||||||| |||||||||||||||||| | ||||||| ||||||
Sbjct 1920 TGGAGTATGTAATCCATCTGATCCGAGAACTATGTTTAAGTATTAATGCCAAACGCAAAC 1979
Query 2020 TTCAAATGAAGCTTTCTT 2037
||||||| ||| ||||||
Sbjct 1980 TTCAAATAAAGATTTCTT 1997
Score = 76.8 bits (41), Expect = 6e-13
Identities = 41/41 (100%), Gaps = 0/41 (0%)
Strand=Plus/Plus
Query 3 CGACTCCTGAGCCTCTCTCTTTCTATCGCTCTTTGATTTTG 43
|||||||||||||||||||||||||||||||||||||||||
Sbjct 1 CGACTCCTGAGCCTCTCTCTTTCTATCGCTCTTTGATTTTG 41
> Ahg483403 jgi|Araly1|483403|fgenesh2_kg.4__2463__AT2G43018.1
Length=1985
Score = 1275 bits (690), Expect = 0.0
Identities = 1228/1485 (83%), Gaps = 48/1485 (3%)
Strand=Plus/Plus
Query 279 TAATAGTAGTAC-TAATTTTAATAATCTTGAGAGTAATTAC-TCCGTTC-ATTG-TTGAA 334
|||||||| | | ||||| |||||||| |||||||| | | | | || |||| | ||
Sbjct 364 TAATAGTAAT-CGTAATTCCAATAATCTCGAGAGTAA-T-CGTTTGATCAATTGAATAAA 420
Query 335 TCATGGAGTCCGGAGGCAAAA--CTAATCGTCAGCTGCGTAAAGCTATTTGCGTCTCAAC 392
||||||||| | ||| |||| || ||||||| |||||| ||||| |||| | ||| |
Sbjct 421 TCATGGAGT-C-TAGGAAAAACTCTGATCGTCAAATGCGTAGAGCTAATTGCTTTTCAGC 478
Query 393 AGACGAGAAGATGAAGAAGAAGAGATCACCTTCAGTGATAGTGATTGGAGGTGG-TATGG 451
| |||| ||||||| | ||| | || || || ||||| ||||| |||||||| | |||
Sbjct 479 TGGTGAGAGGATGAAG-ACAAG-G-TCTCCGTCCGTGATTGTGATCGGAGGTGGTTTTGG 535
Query 452 CTGGAATTTCAGCCGCTCGCACTCTTCAGGATGCTTCCTTTCAGGTTGTTGTATTGGAGT 511
||| |||||||| |||||||||||||| |||||||||||||||||| | ||| ||||||
Sbjct 536 -TGGGATTTCAGCTGCTCGCACTCTTCAAGATGCTTCCTTTCAGGTTATGGTACTGGAGT 594
Query 512 CTCGTGACAGAATTGGTGGACGAGTTCACACTGATTACTCATTTGGTTTTCCAGTTGATC 571
| |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 595 CCCGTGACAGAATTGGTGGACGAGTTCACACTGATTACTCATTTGGTTTTCCTGTTGATC 654
Query 572 TCGGTGCATCTTGGTTGCATGGTGTCTGTAAAGAGAATCCTTTGGCTGCAGTAATTGGTA 631
| |||||||| ||| ||||||| || || |||||||||||| |||| |||||||||| |
Sbjct 655 TTGGTGCATCATGGCTGCATGGAGTATGCAAAGAGAATCCTCTGGCACCAGTAATTGGAA 714
Query 632 GATTAGGATTACCTCTT-TATCGTACTAGCGGTGACAATTCGGTTTTGTATGATCACGAT 690
|| | ||| | || ||| ||||||||||||||||||||||| || |||||||||| |||
Sbjct 715 GACTGGGACTGCC-CTTGTATCGTACTAGCGGTGACAATTCTGTCCTGTATGATCATGAT 773
Query 691 CTTGAGAGTTATGCTCTGTTCGACAAGGCTGGTAACCAAGTTTCTCAAGAGTTGGTGACA 750
|| ||||| ||||||||||| || | || ||| ||||||||| ||||||||||||| ||
Sbjct 774 CTCGAGAGCTATGCTCTGTTTGATATGGATGGCAACCAAGTTCCTCAAGAGTTGGTAACT 833
Query 751 AA-AGTTGGCGAAAATTTTGAGCACATTCTGGAAGAGATCTGTAAAGTCAGGGATGAGCA 809
| | |||||| || |||||||| ||| ||||||||||| ||||||||||||||||||
Sbjct 834 CAGA-TTGGCGTAACTTTTGAGCGTATTTTGGAAGAGATCAATAAAGTCAGGGATGAGCA 892
Query 810 GGACGAAGACATGTCAATAGCTCAAGCCTTT-TCTATTGTATTTAAGAGGAA-CCCTGAA 867
||| | |||||| ||||| |||| || ||| || ||||| ||| ||||| ||| ||
Sbjct 893 GGATGCAGACATTTCAATTTCTCAGGC-TTTCTCAATTGTCTTTGCTAGGAAACCC-GAG 950
Query 868 TTGAGGTTGGAGGGACTTGCCCATAATGTGCTTCAGTGGTACT-TGTGCCGCATGGAAGG 926
||||||| ||||| ||||| || || || ||||| ||||| | | ||||||||||||||
Sbjct 951 CTGAGGTTAGAGGGGCTTGCACACAACGTACTTCAATGGTA-TGTATGCCGCATGGAAGG 1009
Query 927 ATGGTTTGCTGCTGATGCTGAAACCATCTCGGCAAAGTGTTGGGATCAGGAAGAACTGTT 986
|||||||||||| ||||||||||||||||| |||||||||||||| ||||| ||| || |
Sbjct 1010 ATGGTTTGCTGCAGATGCTGAAACCATCTCTGCAAAGTGTTGGGACCAGGAGGAATTGCT 1069
Query 987 GCCCGGTGGACATGGTCTTATGGTTAGAGGGTATCGCCCTGTAATCAACACTCTCTCGAA 1046
||| |||||||| ||||||||||||||||||||||| ||||| ||||||||||| ||||
Sbjct 1070 GCCTGGTGGACACGGTCTTATGGTTAGAGGGTATCGTCCTGTCATCAACACTCTGGCGAA 1129
Query 1047 AGGTCTTGATATACGACTAAGTCACAGAATTACCAAGATT-TCAAGGCGCTACAGTGGTG 1105
|||||||||||| ||| || | ||||| |||| |||||| ||| |||| |||| ||| |
Sbjct 1130 AGGTCTTGATATCCGAGTAGGCCACAGGGTTACTAAGATTGTCA-GGCGTTACAATGGGG 1188
Query 1106 TGAAGGTGACTACAGAGAAA-GGAGACACGTTTGTTGCGGATGCTGCAGTCATTGCTCTT 1164
||||||| || ||||| ||| || || || |||||||||||||||||||||||||| ||
Sbjct 1189 TGAAGGTAACGACAGA-AAACGGGGAAACATTTGTTGCGGATGCTGCAGTCATTGCGGTT 1247
Query 1165 CCTCTAGGAGTCTTAAAGTCAGGAATGATAACA-TTCGAACCAAAGCTTCCACAGTGGAA 1223
||||| ||||||||||| || |||| |||| | |||||||||||||| || |||||||
Sbjct 1248 CCTCTCGGAGTCTTAAAATCTGGAACCATAA-AGTTCGAACCAAAGCTACCTGAGTGGAA 1306
Query 1224 ACAAGAAGCTATCAACGATCTCGGTGTTGGAATCGAGAACAAGATCATATTGA-ATTTTG 1282
||||||||| |||||||| || || || || |||||||||||||||||| | | ||||||
Sbjct 1307 ACAAGAAGCAATCAACGACCTAGGAGTAGGTATCGAGAACAAGATCATACT-ACATTTTG 1365
Query 1283 ACAACGTCTTTTGGCC-AAACGTAGAGTTTTTAGGAGTCGTTGCAGAAACTTCCTACGGA 1341
| || ||||| ||||| ||| ||||||||| ||||||| || || ||||| ||||||||
Sbjct 1366 AGAAAGTCTTCTGGCCGAAA-GTAGAGTTTCTAGGAGTGGTAGCTGAAACCTCCTACGGT 1424
Query 1342 TGCAGTTATTTCTTGAACCTGCATAAAGCCACAAGCCATCCTGTCCTTGTTTACATGCCG 1401
||||||||||| ||||||||||| || |||||| | ||||| || || ||||||||||||
Sbjct 1425 TGCAGTTATTTTTTGAACCTGCACAAGGCCACAGGTCATCCAGTTCTGGTTTACATGCCG 1484
Query 1402 GCTGGTCAGCTCGCTAGAGACATCGAGAAAAAGTCCGATGAAGCAGCTGCAAATTTTGCC 1461
|||||||||||||| | |||||| ||||||| ||| ||||||||||||||||||||||||
Sbjct 1485 GCTGGTCAGCTCGCCAAAGACATTGAGAAAATGTCTGATGAAGCAGCTGCAAATTTTGCC 1544
Query 1462 TT-TTCGCAACTACAGAAAATTCTTCCAGATGCCTCTTCA-CC-GATAAATTAT-TTGGT 1517
| || |||||| |||| ||||||||| ||||| | | | || | | ||| || ||| |
Sbjct 1545 GTCTT-GCAACTCCAGAGAATTCTTCCCGATGCAT-TGCCTCCAGTTCAAT-ATCTTG-T 1600
Query 1518 ATCAAGGTGGGGATCAGACATCAATTCATTGGGATCATATAGCTATGACATTGTGAACAA 1577
||| ||||||||||| || | |||||| ||||||| ||||||||||| || ||| ||
Sbjct 1601 ATCGAGGTGGGGATCGGATGTGAATTCAATGGGATCTTATAGCTATGATATCGTGGGGAA 1660
Query 1578 ACCTCACGACCTCTATGAGAGACTGAGAGTGCCATTGGATAATCT-ATTCTTTGCGGGGG 1636
|||||| || ||||| ||||| || || || ||| ||||||| || ||||||||| || |
Sbjct 1661 ACCTCATGAGCTCTACGAGAGGCTTAGGGTTCCAGTGGATAA-CTTATTCTTTGCCGGTG 1719
Query 1637 AAGCCACTAGCTCAAGCTACCCGGGATCAGTGCACGGTGCTTATTCAACCGGAGTGCTGG 1696
|||| || ||||| |||| |||||| || || ||||| ||||||||||| ||| || |||
Sbjct 1720 AAGCGACAAGCTCGAGCTTCCCGGGCTCGGTTCACGGCGCTTATTCAACTGGATTGATGG 1779
Query 1697 CAGCTGAAGACTGCAGGATGCGTGTGCTTG-AGCGCTATGGAGAG 1740
|||||||||| || |||||||| || ||| |||| ||||| |||
Sbjct 1780 CAGCTGAAGAATGTAGGATGCGAGTT-TTGGAGCGGTATGGTGAG 1823
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 76675663185
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5