BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT4G10440.1
Length=1902
Score E
Sequences producing significant alignments: (Bits) Value
Ahg327211 jgi|Araly1|327211|fgenesh1_pm.C_scaffold_6002431 3049 0.0
Ahg473493 jgi|Araly1|473493|fgenesh2_kg.1__3447__AT1G33170.1 1602 0.0
> Ahg327211 jgi|Araly1|327211|fgenesh1_pm.C_scaffold_6002431
Length=1896
Score = 3049 bits (1651), Expect = 0.0
Identities = 1818/1897 (96%), Gaps = 17/1897 (1%)
Strand=Plus/Plus
Query 1 ATGGCGAAAGAGAACAGTGGTCATCATCACCAAACAGAAGCAAGAAGAAAGAAACTAACT 60
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1 ATGGCGAAAGAGAATAGTGGTCATCATCACCAAACAGAAGCAAGAAGAAAGAAACTAACT 60
Query 61 TTGATTCTTGGTGTAAGTGGACTCTGCATTTTGTTCTATGTTTTAGGTGCATGGCAAGCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 61 TTGATTCTTGGTGTAAGTGGACTCTGCATTTTGTTCTATGTTTTAGGTGCATGGCAAGCC 120
Query 121 AATACCGTCCCATCTTCT-ATCTCGAA-GCTCGGATGCGAGACGCAATCAAA-CC--CTT 175
|||||||||||||||||| | |||||| | ||||||| |||||||||||||| || |||
Sbjct 121 AATACCGTCCCATCTTCTTA-CTCGAAAG-TCGGATGTGAGACGCAATCAAATCCTTCTT 178
Query 176 CTTCGTCCTC-T--TCCTCTTCCTCATC-T-T-CAGAGTCAGCTGAACTAGATTTCAAAA 229
||||||| || | |||||||||||||| | | |||||||||||||| ||||||||||||
Sbjct 179 CTTCGTCGTCCTCGTCCTCTTCCTCATCATCTTCAGAGTCAGCTGAATTAGATTTCAAAA 238
Query 230 GCCATAATCAGATTGAGTTAAAGGAAACAAACCAAACCATTAAGTACTTTGAACCATGTG 289
|| ||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 239 GCTATAATCAGATTGAGTTAAAGGAAACAAACCAAACCATCAAGTACTTTGAACCATGTG 298
Query 290 AATTATCTCTCAGTGAGTACACTCCTTGTGAAGACCGACAAAGAGGAAGAAGATTCGATA 349
| |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 299 AGTTATCTCTCAGTGAGTACACTCCTTGTGAAGATCGACAAAGAGGAAGAAGATTCGATA 358
Query 350 GGAACATGATGAAATATAGAGAAAGACATTGTCCTGT-AAAAGATGAGCTTCTTTATTGT 408
||||||||||||||||||||||||||||||||||| | ||||||||||||||||||||||
Sbjct 359 GGAACATGATGAAATATAGAGAAAGACATTGTCCT-TCAAAAGATGAGCTTCTTTATTGT 417
Query 409 TTGATTCCTCCTCCACCAAACTACAAGATTCCATTT-AAATGGCCACAAAGTAGAGACTA 467
|||||||||||||||||||||||||||||||| ||| |||||||||||||| ||||||||
Sbjct 418 TTGATTCCTCCTCCACCAAACTACAAGATTCC-TTTCAAATGGCCACAAAGCAGAGACTA 476
Query 468 TGCTTGGTATGACAATATCCCTCACAAGGAACTTAGTGTTGAGAAAGCAGTTCAAAACTG 527
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 477 TGCTTGGTATGACAATATCCCTCACAAGGAACTCAGTGTTGAGAAAGCAGTTCAAAACTG 536
Query 528 GATTCAAGTTGAAGGTGACCGCTTTAGATTCCCTGGTGGTGGTACTATGTTTCCTCGTGG 587
||||||||| ||||| ||||| ||||||||||||||||| || || ||||||||||||||
Sbjct 537 GATTCAAGTAGAAGGCGACCGGTTTAGATTCCCTGGTGGCGGAACGATGTTTCCTCGTGG 596
Query 588 AGCTGATGCTTATATCGATGATATTGCTAGGCTTATTCCTCTTACTGATGGTGGAATCAG 647
||| ||||||||||| ||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 597 AGCCGATGCTTATATTGATGATATTGCTAGGCTCATTCCTCTTACTGATGGTGGAATCAG 656
Query 648 AACAGCTATTGACACTGGATGTGGTGTTGCAAGTTTTGGTGCTTACCTCTTGAAGAGAGA 707
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 657 AACAGCTATTGACACTGGATGTGGTGTTGCAAGTTTTGGTGCTTACCTCTTGAAGAGAGA 716
Query 708 CATTATGGCTGTGTCTTTTGCTCCAAGAGACACTCATGAAGCTCAGGTACAGTTTGCTTT 767
||||||||||||||||||||||||||| || |||||||||||||||||||||||||||||
Sbjct 717 CATTATGGCTGTGTCTTTTGCTCCAAGGGATACTCATGAAGCTCAGGTACAGTTTGCTTT 776
Query 768 AGAACGCGGAGTTCCTGCGATAATCGGGATTATGGGATCAAGAAGACTTCCTTATCCAGC 827
|||||| |||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct 777 AGAACGTGGAGTTCCTGCGATAATCGGGATTATGGGATCAAGAAGGCTTCCTTATCCAGC 836
Query 828 TAGAGCTTTTGATCTTGCTCATTGTTCTCGTTGTTTGATCCCTTGGTTTAAAAATGATGG 887
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 837 TAGAGCTTTTGATCTTGCTCATTGTTCTCGTTGTTTGATCCCTTGGTTTAAAAATGATGG 896
Query 888 TTTGTACCTTATGGAGGTCGACCGGGTTTTAAGACCGGGCGGTTACTGGATCCTCTCGGG 947
|||||||||||||||||| ||||||||||||||||||||||||||||||||||| |||||
Sbjct 897 TTTGTACCTTATGGAGGTTGACCGGGTTTTAAGACCGGGCGGTTACTGGATCCTTTCGGG 956
Query 948 ACCACCGATTAACTGGAAACAGTACTGGAGAGGGTGGGAGAGAACAGAGGAGGATTTGAA 1007
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 957 ACCACCGATTAACTGGAAACAGTACTGGAGAGGGTGGGAGAGAACAGAGGAGGATTTGAA 1016
Query 1008 GAAAGAGCAAGATTCAATAGAAGATGTAGCAAAGAGTCTTTGCTGGAAGAAAGTAACTGA 1067
|||||||||||||||||||||||||||||||||||||||||| ||||||||||| |||||
Sbjct 1017 GAAAGAGCAAGATTCAATAGAAGATGTAGCAAAGAGTCTTTGTTGGAAGAAAGTGACTGA 1076
Query 1068 AAAAGGTGACTTATCAATTTGGCAAAAGCCTCTCAATCACATTGAGTGTAAAAAGCTCAA 1127
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1077 AAAAGGTGACTTATCCATTTGGCAAAAGCCTCTCAATCACATTGAGTGTAAAAAGCTCAA 1136
Query 1128 ACAAAACAATAAGTCACCTCCGATATGCAGCTCAGATAACGCGGATTCCGCTTGGTACAA 1187
||||||||||||||| |||||| |||| ||||||||||||||||||| ||||||||||||
Sbjct 1137 ACAAAACAATAAGTCGCCTCCGCTATGTAGCTCAGATAACGCGGATTTCGCTTGGTACAA 1196
Query 1188 AGACTTGGAAACTTGTATAACACCATTACCAGAAACAAACAATCCAGATGATTCAGCAGG 1247
||||||||||||||| ||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 1197 AGACTTGGAAACTTGCATAACACCATTACCAGAAACAAACAATCCAGATGAATCAGCAGG 1256
Query 1248 CGGTGCACTCGAGGATTGGCCAGACCGAGCATTCGCGGTACCTCCAAGAATCATCAGAGG 1307
|||||| || ||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1257 CGGTGCGCTTGAGGATTGGCCAGACCGAGCATTCGCGGTTCCTCCAAGAATCATCAGAGG 1316
Query 1308 AACTATACCAGAAATGAACGCGGAGAAATTTAGAGAAGACAACGAGGTTTGGAAAGAGAG 1367
|||||||||||| ||||||||||||||||| ||||||||||| |||||||||||||||||
Sbjct 1317 AACTATACCAGACATGAACGCGGAGAAATTCAGAGAAGACAATGAGGTTTGGAAAGAGAG 1376
Query 1368 AATAGCACATTACAAGAAGATAGTCCCTGAGCTTTCACATGGAAGATTCAGGAACATTAT 1427
|||| |||||||||||||||||||||| ||||||||||||||||||||||||||||| ||
Sbjct 1377 AATAACACATTACAAGAAGATAGTCCCAGAGCTTTCACATGGAAGATTCAGGAACATAAT 1436
Query 1428 GGACATGAACGCTTTTCTCGGCGGATTCGCTGCTTCCATGCTGAAATATCCCTCATGGGT 1487
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1437 GGACATGAACGCTTTCCTCGGCGGATTCGCTGCTTCCATGCTGAAATATCCCTCATGGGT 1496
Query 1488 CATGAACGTTGTCCCGGTCGATGCAGAGAAACAAACGTTAGGTGTGATCTACGAACGTGG 1547
||||||||||||||| |||||||||||||| ||||| ||||||||||||||||||||||
Sbjct 1497 CATGAACGTTGTCCCAGTCGATGCAGAGAAGCAAACACTAGGTGTGATCTACGAACGTGG 1556
Query 1548 ATTGATAGGGACGTATCAAGATTGGTGTGAAGGATTCTCAACGTATCCAAGAACTTATGA 1607
|||||| || || ||||| || |||||||||||||||||||| |||||||||||||||||
Sbjct 1557 ATTGATTGGAACATATCATGACTGGTGTGAAGGATTCTCAACATATCCAAGAACTTATGA 1616
Query 1608 TATGATTCATGCAGGAGGATTGTTCAGCTTATACGAACATAGGTGTGATTTGACGTTGAT 1667
||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 1617 TATGATTCATGCAGGAGGATTGTTCAGCTTATACGAACACCGGTGTGATTTGACGTTGAT 1676
Query 1668 ATTGTTGGAGATGGATCGAATTTTGAGACCAGAAGGAACAGTTGTGTTGAGAGATAATGT 1727
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1677 ATTGTTGGAGATGGATCGAATATTGAGACCAGAAGGAACAGTTGTGTTGAGAGATAATGT 1736
Query 1728 GGAGACGTTGAATAAGGTAGAGAAGATAGTGAAGGGAATGAAGTGGAAGAGTCAAATTGT 1787
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 1737 GGAGACGTTGAACAAGGTAGAGAAGATAGTGAAGGGAATGAAGTGGAAGAGTCAAATAGT 1796
Query 1788 TGATCATGAGAAAGGTCCTTTTAATCCTGAGAAGATTCTTGTTGCTGTTAAAACTTATTG 1847
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1797 GGATCATGAGAAAGGTCCTTTTAATCCTGAGAAGATTCTTGTTGCTGTTAAAACTTATTG 1856
Query 1848 GACTGGTCAACCTTCTGACAAGaacaacaacaacaac 1884
|||||| ||||||||||| ||||||||||||||||||
Sbjct 1857 GACTGGCCAACCTTCTGAAAAGAACAACAACAACAAC 1893
> Ahg473493 jgi|Araly1|473493|fgenesh2_kg.1__3447__AT1G33170.1
Length=2232
Score = 1602 bits (867), Expect = 0.0
Identities = 1431/1700 (84%), Gaps = 52/1700 (3%)
Strand=Plus/Plus
Query 184 TCT-TCCTCTTCCTCATCTTCAGAGTCAGCTGAACTAGATTTCAAAAGCCATAATCAGA- 241
||| || |||| || |||||||| ||| ||| |||||||| |||||||| | || |
Sbjct 419 TCTCTCGTCTTTGTCCTCTTCAGAACCAGTTGAGTTAGATTTCGAAAGCCATCA-CAAAC 477
Query 242 TTGAGTTAAAGGA-AACAAACCAAACCATTAAGTACTTT-GAACCATGTGA-ATTATCTC 298
| ||| | || || || || ||||| | ||||| ||| || |||||||| | | |||
Sbjct 478 TCGAGCTGAA-GATTACGAATCAAACGGTAAAGTA-TTTCGAGCCATGTGACA-TGTCTT 534
Query 299 TCAGTGAGTACACTCCTTGTGAAGACCGACAAAGAGGAAGAAGATTCGATAGGAACATGA 358
| |||||||||||||| |||||||| ||| | |||||||||||||| |||||||||||||
Sbjct 535 TGAGTGAGTACACTCCATGTGAAGATCGAGAGAGAGGAAGAAGATTTGATAGGAACATGA 594
Query 359 TGAAATATAGAGAAAGACATTGTCC-TGTAAAAGATGAGCTTCTTTATTGTTTGATTCCT 417
||||||| ||||| ||||||||||| | | ||||||||||||||||||||||||||||||
Sbjct 595 TGAAATACAGAGAGAGACATTGTCCTTCT-AAAGATGAGCTTCTTTATTGTTTGATTCCT 653
Query 418 CCTCCACCAAACTACAAGATTCCATTTAAATGGCCACAAAGTAGAGACTATGCTTGGTAT 477
|||||||||||||||||||||||||| |||||||| |||||||||||||||||||||||
Sbjct 654 CCTCCACCAAACTACAAGATTCCATTCAAATGGCCTCAAAGTAGAGACTATGCTTGGTAC 713
Query 478 GACAATATCCCTCACAAGGAACTTAGTGTTGAGAAAGC-AGTTCAAAACTGGATTCAAGT 536
||||| ||||| ||||| || || ||| |||||||||| | |||| || |||||||||||
Sbjct 714 GACAACATCCCACACAAAGAGCTAAGTATTGAGAAAGCTA-TTCAGAATTGGATTCAAGT 772
Query 537 TGAAGGTGACCGCTTTAGATTCCCTGGTGGTGGTACTATGTTTCCTCGTGGAGCTGATGC 596
|||||| || | |||||||||||||||||||| || |||||||| ||||||||||||||
Sbjct 773 TGAAGGAGAAAGATTTAGATTCCCTGGTGGTGGCACAATGTTTCCACGTGGAGCTGATGC 832
Query 597 TTATATCGATGATATTGCTAGGCTTATTCCTCTTACTGATGGTGGAATCAGAACAGCTAT 656
||| || |||||||||||||| || ||||||||||||||||| | || |||||||||||
Sbjct 833 TTACATTGATGATATTGCTAGACTCATTCCTCTTACTGATGGAGCCATTAGAACAGCTAT 892
Query 657 TGACACTGGATGTGGTGTTGCAAGTTTTGGTGCTTACCTCTTGAAGAGAGACATTATGGC 716
||| |||||||||||||| || ||||||||||||||| | |||||||| || ||| ||||
Sbjct 893 TGATACTGGATGTGGTGTGGCGAGTTTTGGTGCTTACTTGTTGAAGAGGGATATTGTGGC 952
Query 717 TGTGTCTTTTGCTCCAAGAGACACTCATGAAGCTCAGGTACAGTTTGCTTTAGAACGCGG 776
| |||| ||||||||||||||||||||||||||||| || || ||||| || ||||| ||
Sbjct 953 TATGTCATTTGCTCCAAGAGACACTCATGAAGCTCAAGTTCAATTTGCGTTGGAACGTGG 1012
Query 777 AGTTCCTGCGATAATCGGGATTATGGGATCAAGAAGACTTCCTTATCCAGCTAGAGCTTT 836
|||||||||||| ||||| ||||||||||||||||| |||||||||||||||||||||||
Sbjct 1013 AGTTCCTGCGATTATCGGTATTATGGGATCAAGAAGGCTTCCTTATCCAGCTAGAGCTTT 1072
Query 837 TGATCTTGCTCATTGTTCTCGTTGTTTGATCCCTTGGTTTAAAAATGATGGTTTGTACCT 896
|||||||||||| ||||||||||||||||| ||||||||| | || |||||||||||| |
Sbjct 1073 TGATCTTGCTCACTGTTCTCGTTGTTTGATTCCTTGGTTTCAGAACGATGGTTTGTAC-T 1131
Query 897 T-ATGGAGGTCGACCGGGTTTTAAGACCGGGCGGTTACTGGATCCTCTCGGGACCACCGA 955
| | || || |||||||||||||||||||||||||| |||||||| ||||| |||||||
Sbjct 1132 TGACCGAAGTAGACCGGGTTTTAAGACCGGGCGGTTATTGGATCCTTTCGGGTCCACCGA 1191
Query 956 TTAACTGGAA-ACAGTACTGGAGAGGGTGGGAGAGAACAGAGGAGGATTTGAAGAAAGAG 1014
| |||||||| | | ||||||| ||| ||||| ||| || | |||||||| ||| |||||
Sbjct 1192 TCAACTGGAAGAAA-TACTGGAAAGGCTGGGAAAGATCACAAGAGGATTTAAAGCAAGAG 1250
Query 1015 CAAGATTCAATAGAAGATGTAGCAAAGA-GTCTTTGCTGGAAGAAAGTAACTGAAAAAGG 1073
|||||||| |||||||||| ||||| || ||||||| ||||||||||| || ||||||||
Sbjct 1251 CAAGATTCTATAGAAGATGCAGCAA-GAAGTCTTTGTTGGAAGAAAGTTACAGAAAAAGG 1309
Query 1074 TGACTTATCAATTTGGCAAAAGCCTC-TCAATCACATTGAGTGTAA-AAAGCTCAAACAA 1131
||| |||||||||||||||||||| | | ||||||||||||||||| ||| ||||||| |
Sbjct 1310 TGATTTATCAATTTGGCAAAAGCC-CATAAATCACATTGAGTGTAACAAA-CTCAAACGA 1367
Query 1132 AA-CA-ATAAGTCACCTCCGATATGCAGC---TCAGATA-ACGCGGATTCCGCTTGGTAC 1185
|| | || || ||||| ||||||| |||||| || || ||| |||||||||
Sbjct 1368 GTTCACA-AA-TCTCCTCCTCTATGCAGTAAATCAGATCTACCCG-ATTTTGCTTGGTAC 1424
Query 1186 AAAGACTTGGAAACTTGTATAACACCATTACCAGAAACAAACAATCCAGATGA-TTCAGC 1244
||||| |||||| ||||| |||||||||| |||||| |||||| |||||| || ||| ||
Sbjct 1425 AAAGATTTGGAATCTTGTGTAACACCATTGCCAGAAGCAAACAGTCCAGACGAATTC-GC 1483
Query 1245 AGGCGGTGCACTCGAGGATTGGCCAGACCGAGCATTCGCGGTACCTCCAAGAATCATCAG 1304
|||||||||| | ||||||||||| |||||||| |||||||| || || || || ||| |
Sbjct 1484 AGGCGGTGCATTGGAGGATTGGCCTGACCGAGCTTTCGCGGTCCCACCTAGGATAATCCG 1543
Query 1305 AGGAACTATACCAGAAATGAACGCGGAGAAATTTAGAGAAGACAACGAGGTTTGGAAAGA 1364
||||||||||| || | || || |||||||| ||||||||||| || || ||||| ||
Sbjct 1544 GGGAACTATACCGGACACCAATGCTGAGAAATTCAGAGAAGACAATGAAGTGTGGAAGGA 1603
Query 1365 GAGAATAGCACATTACAAGA-AGATAGTCCCTGAGCTTTCACATGG-AAGATTCAGGAAC 1422
||||||| || ||||||| | ||||| | |||||||||||| | || |||||||||||||
Sbjct 1604 GAGAATAACATATTACAA-ACAGATAATGCCTGAGCTTTCA-AGGGGAAGATTCAGGAAC 1661
Query 1423 ATTATGGACATGAACGCTTTTCTCGGCGGATTCGCTGCTTCCATGCTGAAATATCCCTCA 1482
|| ||||||||||| |||| ||||| ||||| ||||| | ||| |||||||||| ||
Sbjct 1662 ATAATGGACATGAATGCTTACCTCGGAGGATTTGCTGCGGCAATGATGAAATATCCATCT 1721
Query 1483 TGGGTCATGAACGTTGTCCCGGTCGATGCAGAGAAACAAACGTTAGGTGTGATCTACGAA 1542
||||| ||||| || || || || ||||| ||||| ||||| ||||| || || | |||
Sbjct 1722 TGGGTTATGAATGTGGTTCCTGTGGATGCCGAGAAGCAAACATTAGGGGTTATTTTTGAA 1781
Query 1543 CGTGGATTGATAGGGACGTATCAAGATTGGTGTGAAGGATTCTCAACGTATCCAAGAACT 1602
|| ||||| |||||||| |||||||||||||||||||||||||| |||||||||||||||
Sbjct 1782 CGAGGATTTATAGGGACTTATCAAGATTGGTGTGAAGGATTCTCTACGTATCCAAGAACT 1841
Query 1603 TATGATATGATTCATGCAGGAGGATTGTTCAGC-TT-AT-ACGAACATAGGTGTGATTTG 1659
|||||| |||||||||| || |||||||||||| || || | ||||| |||||||| ||
Sbjct 1842 TATGATTTGATTCATGCTGGTGGATTGTTCAGCATTTATGA-GAACA--GGTGTGATGTG 1898
Query 1660 ACGTTGATATTG-TTGGAGATGGATCGAATTTTGAGACCAGAAGGAACAGTTGTGTTGAG 1718
|| ||| || | ||| |||||||| |||| ||||||||||||||||| || ||||| ||
Sbjct 1899 ACATTGTTACTACTTG-AGATGGATAGAATCTTGAGACCAGAAGGAACCGTGGTGTTTAG 1957
Query 1719 AGATAATGTGGAGACGTTGAATAAGGTAGAGAAG-ATAGTGAAGGGAATGAAGTGGAAGA 1777
||| | |||||| | ||||| ||| || | ||| ||| || ||||||| ||||||||
Sbjct 1958 AGACACTGTGGAAATGTTGACGAAGATACA-AAGCATAACCAATGGAATGAGGTGGAAGA 2016
Query 1778 GTCAAATTGTTG-ATCATGAGAAAGGTCCTTTTAATCCTGAGAAGATTCTTGTTGCTGTT 1836
||| ||| ||| |||||||||||||||||||||| ||||||||||| ||| | |||||
Sbjct 2017 GTCGGATT-TTGGATCATGAGAAAGGTCCTTTTAACCCTGAGAAGATCCTTCTCGCTGT- 2074
Query 1837 AAAA-CTTATTGGACTGGTC 1855
|||| | || ||||| ||||
Sbjct 2075 AAAATCCTACTGGACCGGTC 2094
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 71457299620
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5