BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT5G11730.1
Length=2053
Score E
Sequences producing significant alignments: (Bits) Value
Ahg940653 jgi|Araly1|940653|scaffold_601197.1 1786 0.0
Ahg942179 jgi|Araly1|942179|scaffold_602723.1 808 0.0
Ahg351610 jgi|Araly1|351610|fgenesh1_pg.C_scaffold_6002203 787 0.0
> Ahg940653 jgi|Araly1|940653|scaffold_601197.1
Length=1204
Score = 1786 bits (967), Expect = 0.0
Identities = 1127/1205 (94%), Gaps = 7/1205 (1%)
Strand=Plus/Plus
Query 633 AGCTTTTGGTAGTGTTAAGGATGCAGAATAGGATTGTACAGTTGGAAGAAGGAAAAGAAA 692
|||||||| |||| ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1 AGCTTTTGTTAGTCTTAAGGATGCAGAATAGGATTGTACAGTTGGAAGAAGGAAAAGAAA 60
Query 693 ATGGAA-T-C-ACAGTTCGATCAGGGCATTATAAAGGATTTCCATCGAAACTGCTACTTC 749
|||||| | | | |||||||||||||| ||||| |||||||| ||||||||||| ||||
Sbjct 61 ATGGAATTCCGATTGTTCGATCAGGGCAGTATAAGGGATTTCCGTCGAAACTGCTGCTTC 120
Query 750 TGCTAGGGTTGTTTCTTACATTTTCTGTTACTGTCTTCATCATTAGTGTATCTACTATCA 809
|| ||||||| |||||| | ||||||||||||||||||||||||||||||||||| ||||
Sbjct 121 TGTTAGGGTTTTTTCTTGCTTTTTCTGTTACTGTCTTCATCATTAGTGTATCTACAATCA 180
Query 810 AGTATACTGGAATACAAAGTGTAGTAACCACAGTCACTTCAAGCTTTGTGCCATGCCGTG 869
|||||||||| ||||||||||||||||||||||| |||||||||||||| |||||||| |
Sbjct 181 AGTATACTGGTATACAAAGTGTAGTAACCACAGTGACTTCAAGCTTTGTACCATGCCGCG 240
Query 870 AGGGGGAACCGAATAGTTTGAGTAAATGGATACAACCTCCTGCGGTTTTGATGCATAACA 929
||| ||||||||||||||||||||||||||||| |||||||||||| ||||||||||||
Sbjct 241 AGGATGAACCGAATAGTTTGAGTAAATGGATACAGCCTCCTGCGGTTCTGATGCATAACA 300
Query 930 TGAGCGATGAAGAGCTTCTTTGGCGTGCTTCTTTTTGGCCTCGTAGAAAAGAATATCCGT 989
|||||||||||||||| |||||||||||||||||||||||||| || ||||||||||| |
Sbjct 301 TGAGCGATGAAGAGCTCCTTTGGCGTGCTTCTTTTTGGCCTCGGAGGAAAGAATATCCCT 360
Query 990 TTAAACGGGTTCCAAAGGTTGCGTTTATGTTCTTGACTAAAGGCCCCTTACCGCTTGCT- 1048
||||||| ||||| ||| |||||||||||||||| |||||||||||||| |||||||||
Sbjct 361 TTAAACGAGTTCCGAAGATTGCGTTTATGTTCTTAACTAAAGGCCCCTTGCCGCTTGCTA 420
Query 1049 TCGCTTTGGGAGAGGTTCTTGAAGGGTCACAAAGGGCTTTACTCGGTTTACCTTCATCCA 1108
| |||||||||||||||||| |||||||| |||||||||||||||||||||||||||||
Sbjct 421 T-GCTTTGGGAGAGGTTCTTAAAGGGTCATAAAGGGCTTTACTCGGTTTACCTTCATCCC 479
Query 1109 CACCCTTCTTTTACAGCCAAGTTTCCAGCTAGCTCTGTTTTCCACCGGAGGCAAATTCCA 1168
||||| |||||||||||||| |||||| |||||||||||||| |||||||||| || ||
Sbjct 480 CACCCATCTTTTACAGCCAAATTTCCATCTAGCTCTGTTTTCTACCGGAGGCAGATACCG 539
Query 1169 AGCCAGGTTGCAGAATGGGGGAGAATGTCCATGTGCGATGCAGAGAAGCGGCTTCTTGCC 1228
||||||||||||||||||||||||||||| ||||| |||||||||||||||||||| |||
Sbjct 540 AGCCAGGTTGCAGAATGGGGGAGAATGTCTATGTGTGATGCAGAGAAGCGGCTTCTCGCC 599
Query 1229 AACGCCCTGCTTGATGTGTCCAACGAATGGTTTGTTCTCGTCTCAGAGTCATGCATTCCA 1288
||||| ||||| ||| | ||||||||||||||||||||||||||||||||||||||||||
Sbjct 600 AACGCGCTGCTCGATATCTCCAACGAATGGTTTGTTCTCGTCTCAGAGTCATGCATTCCA 659
Query 1289 CTCTACAACTTCACAACCATTTACAGCTACTTAAGCCGATCAAAACACAGCTTCATGGGT 1348
|||| ||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 660 CTCTTCAACTTCACAACCATCTACAGCTACTTAAGCCGATCAAAACACAGCTTCATGGGT 719
Query 1349 GCTTTTGATGACCCTGGACCGTTTGGGCGTGGCCGCTACAACGGGAACATGGAACCTGAA 1408
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 720 GCTTTTGATGACCCTGGACCGTTTGGGCGTGGCCGCTACAACGGGAACATGGAACCTGAA 779
Query 1409 GTTCCCCTTACCAAATGGCGAAAAGGGTCTCAATGGTTTGAGGTTAACCGGGACCTGGCA 1468
||||||||| |||||||||||||||||||||||||||||||||||||| ||||||| |||
Sbjct 780 GTTCCCCTTTCCAAATGGCGAAAAGGGTCTCAATGGTTTGAGGTTAACAGGGACCTCGCA 839
Query 1469 GCTACCATTGTCAAAGACACATTGTATTACCCGAAATTCAAAGAGTTCTGCAGGCCTGCT 1528
||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 840 GCTACCATTGTCAAAGACACATTGTATTACCCGAAATTCAAAGAGTTCTGCCGGCCTGCG 899
Query 1529 TGCTATGTGGACGAGCATTACTTCCCAACAATGTTGACGATTGAGAAACCAACAGTCTTG 1588
||||||||||||||||||||||||||||| |||||||| |||||||||||||| ||||||
Sbjct 900 TGCTATGTGGACGAGCATTACTTCCCAACGATGTTGACAATTGAGAAACCAACGGTCTTG 959
Query 1589 GCTAACCGGAGCTTGACATGGGTGGACTGGTCGAGGGGCGGTCCCCACCCAGCTACGTTT 1648
|||||||| ||||||||||||||||||||||||||||| || |||||||||||||| |||
Sbjct 960 GCTAACCGAAGCTTGACATGGGTGGACTGGTCGAGGGGAGGGCCCCACCCAGCTACATTT 1019
Query 1649 GGGCGATCGGACATTACAGAAAACTTCTTTGGAAAA-ATCTTTGATGGAAGAAACTGCAG 1707
|||||||| ||||||||||| || ||||||| ||| |||||||||||||||||||||||
Sbjct 1020 GGGCGATCTGACATTACAGAGAAGTTCTTTG-AAAGGATCTTTGATGGAAGAAACTGCAG 1078
Query 1708 TTACAATGGTCGCAACACTTCCATGTGTTATCTCTTTGCGAGGAAGTTTGCTCCGAGTGC 1767
|||||||||| |||||||||||||||||||||||||||| |||||||||||||||||| |
Sbjct 1079 TTACAATGGTGGCAACACTTCCATGTGTTATCTCTTTGCAAGGAAGTTTGCTCCGAGTAC 1138
Query 1768 TTTGGAGCCTTTGCTTCACATTGCTCCCAAGATTCTTGGATTTTGAGTAGTTTAGTTATG 1827
| ||||||||||||||||||||||||||||| |||||||||||||||||||||||| |||
Sbjct 1139 TCTGGAGCCTTTGCTTCACATTGCTCCCAAGGTTCTTGGATTTTGAGTAGTTTAGTAATG 1198
Query 1828 TCTCT 1832
|||||
Sbjct 1199 TCTCT 1203
> Ahg942179 jgi|Araly1|942179|scaffold_602723.1
Length=1201
Score = 808 bits (437), Expect = 0.0
Identities = 961/1207 (80%), Gaps = 63/1207 (5%)
Strand=Plus/Plus
Query 642 TAGTGTTAAGGATGCAGAATAGGATTGTACAGTTGGAAGAAGGAAAAGAAAATGGAATCA 701
||||| |||||||||||| |||| || | |||||||||||||| ||||| | || | | |
Sbjct 11 TAGTG-TAAGGATGCAGAGTAGGGTTATTCAGTTGGAAGAAGGTAAAGATATTGTAGTTA 69
Query 702 CAGTTCGATCAGGG-C--ATTATA-AAGG-ATTTCC-ATCGAAACTGCTACTTC-TGCTA 754
| | || | ||| | || | |||| ||||| || || | || | || |||||
Sbjct 70 C-GGGCG-GCCGGGCCGGGCTAAATAAGGCGTTTCCTTTC-AAGTTACTTC-TCTTGCTA 125
Query 755 GGGTTGTTTCTTA-CATTTT-CTGTTACTGTCTTCATCATTAGTGTATCTACTA-TCAAG 811
|| || |||| || | |||| ||||| | | ||| ||||||||||||| || | |||||
Sbjct 126 GGATTTTTTC-TAGC-TTTTACTGTTGTTTTGTTCTTCATTAGTGTATCCAC-ATTCAAG 182
Query 812 TA-TACTGGAATACAA-AGTGTAGTAACCACAGTCACTTCAAGCTTTGTGCCATGCCGTG 869
|| || |||||| ||| |||||||| || ||||||| || || |||||||| |||| |
Sbjct 183 TATTA-TGGAAT-CAATAGTGTAGTGACTTCAGTCACGTCTAGTTTTGTGCCTTGCCACG 240
Query 870 AG-GGGGAACCGAATAGTTTGAGTAAATGGA-TACAACCTCCTGCGGTTTTGATGCATAA 927
|| ||| || || || | |||||||| || |||||||| |||| | ||||||||
Sbjct 241 AGAAAAGAAACG-AT--CTTCA-TAAATGGATTA-AACCTCCTATGGTTCTCATGCATAA 295
Query 928 CATGAGCGATGAAGAGCTTCTTTGGCGTGCTTCTTT-TTGGCCT-CGTAGAAAAGAATAT 985
|||||||||||||| |||||||||||||| ||||| | |||| | || ||||| |||
Sbjct 296 TATGAGCGATGAAGAACTTCTTTGGCGTGCGTCTTTCAT-GCCTAAG-AGGAAAGAGTAT 353
Query 986 CCGTTTAA-ACGGGTTCC-AAAGGTTGCGTTTATGTTCTTGACTAA-AGGCCCCTTACCG 1042
|||||||| | |||| || ||| | ||||||||||||||||| || || || || |||
Sbjct 354 CCGTTTAATA-GGGTGCCGAAA-ATCGCGTTTATGTTCTTGAC-AATGGGTCCTTTGCCG 410
Query 1043 CTTGCTTCGCTTTGGGAGAGGTTCTTGAAGGGTCACAAAG-GGCTTTACTCGGTTTACCT 1101
||||| |||||||||| ||||| || |||||||| || || ||| || |||||| |
Sbjct 411 CTTGCACCGCTTTGGGAAAGGTTATTAAAGGGTCA-TGAGAAGCATTATTCAGTTTACAT 469
Query 1102 TCATCCACACCCT-TC-TTTTACAGCCAAGTTTCCAGCTAGCTCTGTTTTCCACCGGAGG 1159
||| || || | || | || ||||||||||||||||| |||||||||| |||| |||
Sbjct 470 CCATTCA-ACAGTATCGTCTT-CAGCCAAGTTTCCAGCTTCCTCTGTTTTCTACCGTAGG 527
Query 1160 CAAATTCCAAGCCAGGTTGCAGAATGGGGGAGAATGTCCATGTGCGATGCAGAGAAGCGG 1219
|| || ||||| ||||||||||||||||| |||||| | |||||||||||||||| ||||
Sbjct 528 CATATACCAAGTCAGGTTGCAGAATGGGGAAGAATGACAATGTGCGATGCAGAGAGGCGG 587
Query 1220 CTTCTTGCCAACGCCCTGCTTGATGTGTCCAACGAATGGTTTGTTCTCGTCTCAGAGTCA 1279
||||||||||| || || || ||| | || |||||||||||||||||| |||| ||||||
Sbjct 588 CTTCTTGCCAATGCGCTACTCGATATCTCAAACGAATGGTTTGTTCTCCTCTCCGAGTCA 647
Query 1280 TGCATTCCACTCTACAACTTCACAACCATTTACAGC-TACTTAAGCC-GATCAAAACACA 1337
|||||||| |||| |||||||||||| || |||| | ||| ||| || |||||| ||||
Sbjct 648 TGCATTCCCCTCTTCAACTTCACAACTATCTACA-CTTACATAA-CCAAATCAAAGCACA 705
Query 1338 GCTTCATGGGTGCTTTTGATGACCCTGGACCGTTTGGGCGTGGCCGCTACAACGGGAACA 1397
|||| |||||| | |||||||| ||| | || | ||| || ||||||||| |||| ||||
Sbjct 706 GCTTTATGGGTTCATTTGATGATCCTAGTCCCTATGGCCGAGGCCGCTACCACGGCAACA 765
Query 1398 TGGAACCTGAAGTTCCCCT-TACCAAATGGCGAAAAGGGTCTCAATGGTTTGAGGTTAAC 1456
||| ||||||||| || | ||| ||||| |||||||||||||||||||||||||||||
Sbjct 766 TGGCCCCTGAAGTTTCCATAAACC-AATGGAGAAAAGGGTCTCAATGGTTTGAGGTTAAC 824
Query 1457 CGGGACCTGGCAGCTACCATTGTCAAAGACACATTGTATTACCCGAAATTCAAAGAGTTC 1516
||||| || || | | ||||||||||||| || ||||||||||| ||||||||| ||||
Sbjct 825 CGGGAGCTTGCGGTTTCCATTGTCAAAGATACCTTGTATTACCCTAAATTCAAACAGTTT 884
Query 1517 TGCAGGCCTGCTTGCTATGTGGACGAGCATTACTTCCCAACAATGTTGACGATTGAGAAA 1576
|| | |||||||| || |||||||||||||| ||||||||||||||||| |||||||||
Sbjct 885 TGTAAACCTGCTTGTTACGTGGACGAGCATTATTTCCCAACAATGTTGACAATTGAGAAA 944
Query 1577 CCAACAGTCTTGGCTAACCGGAGCT-TGACATGGGTGGACTGGTCGAGGGGCGGTCCCCA 1635
|| | | |||||| || || || | |||| ||||| ||||||||||| || ||| ||||
Sbjct 945 CCGGCGGCCTTGGCCAATCGAAG-TGTGACTTGGGTCGACTGGTCGAGAGGTGGTGCCCA 1003
Query 1636 CCCAGCTACGTTTGG-GCGATCG-GACATTACAGAAAACTTCTTTGGAAAAAT-CTTTGA 1692
||||||||| ||||| || | | ||||| | || | ||||||| | | || |
Sbjct 1004 CCCAGCTACATTTGGAGC-A-CAAGACATCAGTGAGGAGTTCTTTGCATGGGTTCTCAAA 1061
Query 1693 TGGAAGA-AACTGCAGTTACAATGGTCGCAACACTTCCATGTGTTATCTCTTTGCGAGGA 1751
|| || ||||||| |||||||||| || |||| |||||||||||||||||||| || |
Sbjct 1062 -GGT-GATAACTGCACTTACAATGGTGGCTACACATCCATGTGTTATCTCTTTGCTAGAA 1119
Query 1752 AGTTTGCTCCGAGTGCTTTGGAGCCTTTGCTTCACATTGCTCCCAAGATTCTTG-GATTT 1810
||||| ||||||||||||| ||||||||| |||| ||||||||||||||||| | | |||
Sbjct 1120 AGTTTTCTCCGAGTGCTTTAGAGCCTTTGATTCAAATTGCTCCCAAGATTCT-GAGTTTT 1178
Query 1811 TGAGTAG 1817
|||||||
Sbjct 1179 TGAGTAG 1185
> Ahg351610 jgi|Araly1|351610|fgenesh1_pg.C_scaffold_6002203
Length=1161
Score = 787 bits (426), Expect = 0.0
Identities = 876/1088 (81%), Gaps = 51/1088 (5%)
Strand=Plus/Plus
Query 750 TGCTAGGGTTGTTTCTTA-CATTTT-CTGTTACTGTCTTCATCATTAGTGTATCTACTA- 806
||||||| || |||| || | |||| ||||| | | ||| ||||||||||||| || |
Sbjct 101 TGCTAGGATTTTTTC-TAGC-TTTTACTGTTGTTTTATTCTTCATTAGTGTATCCAC-AT 157
Query 807 TCAAG-TATACTGGAATACAA-AGTGTAGTAACCACAGTCACTTCAAGCTTTGTGCCATG 864
||||| | || |||||| ||| |||||||| || ||||||| || || |||||||| ||
Sbjct 158 TCAAGTTTTA-TGGAAT-CAATAGTGTAGTGACTTCAGTCACGTCTAGTTTTGTGCCTTG 215
Query 865 CCGTGAG-GGGGAACCGAATAGTTTGAGTAAATGGATACAACCTCCTGCGGTTTTGATGC 923
|| ||| ||| || || || | ||||||||| |||||||| |||| | ||||
Sbjct 216 CCACGAGAAAAGAAACG-AT--CTTCA-TAAATGGATTGAACCTCCTATGGTTCTCATGC 271
Query 924 ATAACATGAGCGATGAAGAGCTTCTTTGGCGTGCTTCTTT-TTGGCCTCGTAGA--AAAG 980
|||| |||||||||||||| |||||||||||||| ||||| | |||| ||| ||||
Sbjct 272 ATAATATGAGCGATGAAGAACTTCTTTGGCGTGCGTCTTTCAT-GCCT--AAGACGAAAG 328
Query 981 AATATCCGTTTAA-ACGGGTTCC-AAAGGTTGCGTTTATGTTCTTGACTAA-AGGCCCCT 1037
| | ||||||||| | |||| || ||| | ||||||||||||||||| || || || |
Sbjct 329 AGTTTCCGTTTAATA-GGGTGCCGAAA-ATCGCGTTTATGTTCTTGAC-AATGGGTCCTT 385
Query 1038 TACCGCTTGCTTCGCTTTGGGAGAGGTTCTTGAAGGGTCACAAAG-GGCTTTACTCGGTT 1096
| |||||||| |||||||||| ||||| || |||||||| || || ||| || |||
Sbjct 386 TGCCGCTTGCACCGCTTTGGGAAAGGTTATTAAAGGGTCA-TGAGAAGCATTATTCAGTT 444
Query 1097 TACCTTCATCCACACCCT-TCTT-TTACAGCCAAGTTTCCAGCTAGCTCTGTTTTCCACC 1154
||| | ||| ||| | | |||| || |||||||||||| |||| |||||||||| |||
Sbjct 445 TACATCCATTCAC-CAGTATCTTCTT-CAGCCAAGTTTCAAGCTTCCTCTGTTTTCTACC 502
Query 1155 GGAGGCAAATTCCAAGCCAGGTTGCAGAATGGGGGAGAATGTCCATGTGCGATGCAGAGA 1214
| ||||| || ||||| ||||||||||||||||| |||||| | ||||||||||||||||
Sbjct 503 GTAGGCATATACCAAGTCAGGTTGCAGAATGGGGAAGAATGACAATGTGCGATGCAGAGA 562
Query 1215 AGCGGCTTCTTGCCAACGCCCTGCTTGATGTGTCCAACGAATGGTTTGTTCTCGTCTCAG 1274
||||||||||||||| || || || ||| | || |||||||||||||||||| |||| |
Sbjct 563 GGCGGCTTCTTGCCAATGCGCTACTCGATATCTCAAACGAATGGTTTGTTCTCCTCTCCG 622
Query 1275 AGTCATGCATTCCACTCTACAACTTCACAACCATTTACAGC-TACTTAAGCC-GATCAAA 1332
||||||||||||| |||| |||||||||||| || |||| | ||| ||| || ||||||
Sbjct 623 AGTCATGCATTCCCCTCTTCAACTTCACAACTATCTACA-CTTACATAA-CCAAATCAAA 680
Query 1333 ACACAGCTTCATGGGTGCTTTTGATGACCCTGGACCGTTTGGGCGTGGCCGCTACAACGG 1392
|||||||| |||||| | |||||||| ||| | || | ||| || ||||||||| ||||
Sbjct 681 GCACAGCTTTATGGGTTCATTTGATGATCCTAGTCCATATGGCCGAGGCCGCTACCACGG 740
Query 1393 GAACATGGAACCTGAAGTTCCCCT-TACCAAATGGCGAAAAGGGTCTCAATGGTTTGAGG 1451
||||||| ||||||||| || | ||| ||||| ||||||||||||||||||||||||
Sbjct 741 CAACATGGCCCCTGAAGTTTCCATAGACC-AATGGAGAAAAGGGTCTCAATGGTTTGAGG 799
Query 1452 TTAACCGGGACCTGGCAGCTACCATTGTCAAAGACACATTGTATTACCCGAAATTCAAAG 1511
|||||||||| || |||| | ||||||||||||| || ||||||||||| |||||||||
Sbjct 800 TTAACCGGGAGCTTGCAGTTTCCATTGTCAAAGATACCTTGTATTACCCTAAATTCAAAC 859
Query 1512 AGTTCTGCAGGCCTGCTTGCTATGTGGACGAGCATTACTTCCCAACAATGTTGACGATTG 1571
|||| || | |||||||| || |||||||||||||| ||||||||||||||||| ||||
Sbjct 860 AGTTTTGTAAACCTGCTTGTTACGTGGACGAGCATTATTTCCCAACAATGTTGACAATTG 919
Query 1572 AGAAACCAACAGTCTTGGCTAACCGGAGCT-TGACATGGGTGGACTGGTCGAGGGGCGGT 1630
||||||| | | |||||| ||||| || | |||| ||||| ||||||||||| || |||
Sbjct 920 AGAAACCGGCGGCCTTGGCCAACCGAAG-TGTGACTTGGGTCGACTGGTCGAGAGGTGGT 978
Query 1631 CCCCACCCAGCTACGTTTGG-GCGATCG-GACATTACAGAAAACTTCTTTGGAAAAAT-C 1687
||||||||||||| ||||| || | | ||||| | || | ||||||| | | |
Sbjct 979 GCCCACCCAGCTACATTTGGAGC-A-CAAGACATCAGTGAGGAGTTCTTTGCATGGGTTC 1036
Query 1688 TTTGATGGAAGA-AACTGCAGTTACAATGGTCGCAACACTTCCATGTGTTATCTCTTTGC 1746
| | || || ||||||| |||||||||| || |||| ||||||||||||||||||||
Sbjct 1037 TCAAA-GGT-GATAACTGCACTTACAATGGTGGCTACACATCCATGTGTTATCTCTTTGC 1094
Query 1747 GAGGAAGTTTGCTCCGAGTGCTTTGGAGCCTTTGCTTCACATTGCTCCCAAGATTCTTG- 1805
|| |||||| ||||||||||||| ||||||||| |||| ||||||||||||||||| |
Sbjct 1095 TAGAAAGTTTTCTCCGAGTGCTTTAGAGCCTTTGATTCAAATTGCTCCCAAGATTCT-GA 1153
Query 1806 GATTTTGA 1813
| ||||||
Sbjct 1154 GTTTTTGA 1161
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 77208926615
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5