BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT5G13590.1
Length=5101
Score E
Sequences producing significant alignments: (Bits) Value
Ahg940824 jgi|Araly1|940824|scaffold_601368.1 2471 0.0
> Ahg940824 jgi|Araly1|940824|scaffold_601368.1
Length=3696
Score = 2471 bits (1338), Expect = 0.0
Identities = 1481/1550 (96%), Gaps = 10/1550 (1%)
Strand=Plus/Plus
Query 2231 CAGGTG-AAACTGAAGGCCGGATAGTTCAGGATGGTGAAGGAACGTCAGGTGTCTCTACA 2289
|||||| |||| ||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct 2149 CAGGTGAAAAC-GAAGGCCGGATAGTTCAGGATGGTGAAGGAATGTCAGGTGTCTCTACA 2207
Query 2290 GTGTCAGGCGGCATTGAAAACCCTGAAATAGTAGATAACAGTAGTCCAGTTTCACT-CAA 2348
|||||| |||||||||||||||||||||||||||||||||| |||||||||||| | |||
Sbjct 2208 GTGTCATGCGGCATTGAAAACCCTGAAATAGTAGATAACAGCAGTCCAGTTTCA-TACAA 2266
Query 2349 GGCAGAAATGTCTACTATTGACAATGATTCTCCTATGGAGTGCAGTGACGGTAGTCAGAG 2408
|||||||||||||||||||||||||||| ||||| ||||||||||||||||||||||||
Sbjct 2267 GGCAGAAATGTCTACTATTGACAATGATCCTCCTGCGGAGTGCAGTGACGGTAGTCAGAG 2326
Query 2409 TCGAATTATAAACTTAACTCAGG----T--TAAATCTCCAGTTAAGGCACTAGATGCTTC 2462
||||||||||||||||||||||| | |||||||||||| |||||||||||||||||
Sbjct 2327 TCGAATTATAAACTTAACTCAGGTTTCTAATAAATCTCCAGTGAAGGCACTAGATGCTTC 2386
Query 2463 AGGCAGCTTTGTGCCACCCCGAATGGAAAGAGATAGATTTCATGATTTCCCACTCGAACC 2522
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2387 AGGCAGCTTTGTGCCACCCCGAATGGAAAGAGATAGATTTCATGATTTCCCACTCGAACC 2446
Query 2523 GCGGGAATATACTTTCAGAGGGAGTGATGAATCCTGCAAATTCTCGCGTGAGAGGTACCA 2582
|||| |||||| |||||||| |||||||||||||| ||||||||||||||||||||||||
Sbjct 2447 GCGGAAATATATTTTCAGAGAGAGTGATGAATCCTACAAATTCTCGCGTGAGAGGTACCA 2506
Query 2583 TGGCAGAATTATGAGAAGCCCAAGGTTAAATTTCATACCTGACAGAAGGAGATTACCTGA 2642
||||| ||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2507 TGGCAAAATCATGAGAAGCCCAAGGTTAAATTTCATACCTGACAGAAGGAGATTACCTGA 2566
Query 2643 TAACACAGAAAGCAATCTGCATGACCAGGACACaaaaaaaTTTGAGTTTGATAATCATGG 2702
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2567 TAACACAGAAAGCAATCTGCATGACCAGGACACAAAAAATTTTGAGTTTGATAATCATGG 2626
Query 2703 AAACACTCGTCGGGGTGGTGCTTTTATGAGTAATTTTCAGAGAGGGAGACGGCCTGCAAA 2762
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2627 AAACACTCGTCGGGGTGGTGCTTTTATGAGTAATTTTCAGAGAGGGAGACGGCCTGCAAA 2686
Query 2763 TGATGGAGTTACACCATATGCTCACTCCTTTCCGAGAAGATCCCCTAGCTTTTCATATAA 2822
||||| ||| ||||| ||| |||||||||||||||||||| |||||||||||| |||
Sbjct 2687 TGATGAAGTAACACCCTATTCTCACTCCTTTCCGAGAAGAAATTCTAGCTTTTCATTTAA 2746
Query 2823 TAGAGGACCAACAAATAAAGAGGATACATCTGCATTTCACGGATTTAGAGATGGTGAAAA 2882
||||||||||||||||||||||||| |||||| |||||||||||||||||||||||||||
Sbjct 2747 TAGAGGACCAACAAATAAAGAGGATGCATCTGTATTTCACGGATTTAGAGATGGTGAAAA 2806
Query 2883 ATTCACAAGGGGATTACAATGCAACAACACAGAACCACTGTTTATGAATCACCAACGTCC 2942
||||||||||||||||||||| ||||||||||||||| |||||||||||| |||||||||
Sbjct 2807 ATTCACAAGGGGATTACAATGTAACAACACAGAACCAATGTTTATGAATCCCCAACGTCC 2866
Query 2943 ATATCGAGGTCGGAGTGGTTTTGCTCGAGGACGAACAAAGTTTGTAAACAACCCCAAACG 3002
||||| |||||||||||||||| |||||||||||||||||||| ||||||| ||||||||
Sbjct 2867 ATATCAAGGTCGGAGTGGTTTTACTCGAGGACGAACAAAGTTTTTAAACAATCCCAAACG 2926
Query 3003 AGATTTTCCTGGATTTCGTTCACGATCTCCAGTTAGATCAAGAGAAAGATCAGATGGTTC 3062
|| ||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 2927 AGGTTTTCCTGGATTTCGTTCACGATCTCCAGTTAGATCAAGAGAACGATCAGATGGTTC 2986
Query 3063 ATCCTCGTCTTTCAGGAATAGATCACAGGAAGAGTTCAGTGGGCATACAGACTTTTCTCA 3122
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2987 ATCCTCGTCTTTCAGGAACAGATCACAGGAAGAGTTCAGTGGGCATACAGACTTTTCTCA 3046
Query 3123 TCGAAGATCACCCTCAGGTTACAAAGTGGAGAGGATGAGCTCGCCTGACCATTCTGGTTA 3182
||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 3047 TCGAAGATCACCCTCAGGTTACAAAATGGAGAGGATGAGCTCGCCTGACCATTCTGGTTT 3106
Query 3183 TTCAAGAGAAATGGTTGTCAGAAGACACAATTCTCCACCTTTCTCGCATAGACCATCGAA 3242
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 3107 TTCAAGGGAAATGGTTGTCAGAAGACACAATTCTCCACCTTTCTCGCATAGACCATCAAA 3166
Query 3243 TGCTGGAAGGGGCCGGGGTTATGCAAGGGGCCGAGGTTATGTAAGGGGTCGAGGTTATGG 3302
|||||||||||| ||||||||| |||||||||||||||||| ||||||||||||||||||
Sbjct 3167 TGCTGGAAGGGGACGGGGTTATCCAAGGGGCCGAGGTTATGCAAGGGGTCGAGGTTATGG 3226
Query 3303 AAGAGATGGCAACTCATTTAGGAAACCATCTGATCATGTTGTACATAGAAACCATGGAAA 3362
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 3227 AAGAGATGGCAACTCATTTAGGAAACCATCTGATCGTGTTGTACATAGAAACCATGGAAA 3286
Query 3363 CATGAATAACTTGGATCCTCGAGAAAGGGTTGACTATAGTGATGATTTCTTTGAAGGTCA 3422
| |||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 3287 CTTGAATAACTTGGATCCTCGAGAAAGGGTCGACTATAGTGATGATTTCTTTGAAGGTCC 3346
Query 3423 AATTCATTCTGAACGATTTGGTGTTGATGTTAATGCTGAGAGAAGACGATTTGGTTATAG 3482
||||||||| |||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 3347 CATTCATTCTAAACGATTTGGTGTTGATGTTAACGCTGAGAGAAGACGATTTGGTTATAG 3406
Query 3483 ACATGATGGTACCAGCAGCTCTTTTAGACCATCTTTTAACAATGATGGTTGTGCACCTAC 3542
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3407 ACATGATGGTACCAGCAGCTCTTTTAGACCATCTTTTAACAATGATGGTTGTGCACCTAC 3466
Query 3543 TAATGTAGAGAATGACCCTGATGCTGTGAGGTTCCAACAAGACCCTCGTATTAAAATTGA 3602
|||||||||| ||||||||||||||||||||||| ||| ||||||| ||||||||||||
Sbjct 3467 TAATGTAGAGCATGACCCTGATGCTGTGAGGTTCGGACAGGACCCTCATATTAAAATTGA 3526
Query 3603 AGAACAAGGGAGTTTAATGGAAATTGATGGAGAAAATAAGAACTCAACTGAGAATGCATC 3662
|||||||||||||||||||||||||||||| |||||||||| ||||||||| |||||||
Sbjct 3527 AGAACAAGGGAGTTTAATGGAAATTGATGGGGAAAATAAGATTTCAACTGAGGATGCATC 3586
Query 3663 TGGAAGAACTAAGAATATGGAAGAGGAAGAAACTTCAAAGAACAGTAAAATTTGGCAACC 3722
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 3587 TGGAAGAACTAAGAATATGGAAGAGGAGGAAACTTCAAAGAACAGTAAAATTTGGCAACC 3646
Query 3723 GGATGAGCTCGGTGGTGATGGTTTTTAAAATAAAGGTTAGTATTCAATTT 3772
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3647 GGATGAGCTCGGTGGTGATGGTTTTTAAAATAAAGGTTAGTATTCAATTT 3696
Score = 1295 bits (701), Expect = 0.0
Identities = 898/990 (91%), Gaps = 25/990 (3%)
Strand=Plus/Plus
Query 1258 GCTGGGAATGTGTGTTCAGTGAACCTTAGGACTGTGAAGTCAGAAATCGTTGAAGAGAGT 1317
|||||||||||| ||| |||||||||||||||||||||||||||||| |||||||||||
Sbjct 1098 GCTGGGAATGTGAATTCGGTGAACCTTAGGACTGTGAAGTCAGAAATCATTGAAGAGAGT 1157
Query 1318 GTTAGGCAGGCAAC--AG-AGAGTACTCAAGTTTCTCCAATCGGGCTATCTATTAAAGGA 1374
|||||||||| ||| || ||||| ||||||| | |||||| ||||||||||||||||||
Sbjct 1158 GTTAGGCAGGAAACTGAGAAGAGTGCTCAAGTCTGTCCAATTGGGCTATCTATTAAAGGA 1217
Query 1375 CTGAAACATGAGGGTATTGGCAGATTCAGCCAAGGAAATAGTCCCTCATTTGGCATTTTG 1434
|||||||||||| | ||||||||||||||||||||||||||||||||| || |||||||
Sbjct 1218 GTGAAACATGAGGTTGTTGGCAGATTCAGCCAAGGAAATAGTCCCTCATCTGTCATTTTG 1277
Query 1435 AAGACAGTGGTTCCTATATCAATAAAGGCTGAGCCAAATACCTTCTCTCAATCAGAAGTT 1494
||| ||||||||||||||||||||||||||||||||||||||||| | ||||| ||||||
Sbjct 1278 AAGCCAGTGGTTCCTATATCAATAAAGGCTGAGCCAAATACCTTCACCCAATCGGAAGTT 1337
Query 1495 TTCAATAGGAAAGATGGAATGTTGAATCATCCCCATACCCCAATAATGCAATCAAATGAG 1554
|||||||||||||||||||||||||||||||||||||| |||||| ||||||||||||||
Sbjct 1338 TTCAATAGGAAAGATGGAATGTTGAATCATCCCCATACACCAATAGTGCAATCAAATGAG 1397
Query 1555 ATCCCTGATTTACCTACAAGTTCTACGCCATATCAGAAGGATAAATATTTACCTTGTTCA 1614
||||||||||||||||||||||||||||||||||||||||||| | || ||||||||||
Sbjct 1398 ATCCCTGATTTACCTACAAGTTCTACGCCATATCAGAAGGATACACATATACCTTGTTCT 1457
Query 1615 AATGGTATCAGCAATGCACCAATGCCCTT-GAGTGGAATGACAATAATTCCAGGCGTTCA 1673
|||||||||||||||||||||||| |||| || ||||||||||||| |||||||||||||
Sbjct 1458 AATGGTATCAGCAATGCACCAATGTCCTTAGA-TGGAATGACAATAGTTCCAGGCGTTCA 1516
Query 1674 GAGTGATCCTGACTGTACATCAAAAGAAAATTCGGGCCAGAGTAGCAGTTTAGCTAATGG 1733
|| ||||||||||||||||| |||||||||||| || |||||||||||| |||||||
Sbjct 1517 GATTGATCCTGACTGTACATTAAAAGAAAATTCAGGTCAGAGTAGCAGTGTAGCTAA--- 1573
Query 1734 TAAATTACGCGAAGTGCTGAAACATGGTGGAGTTTACACGACTTATTC-TG----G-TCA 1787
| ||| |||||||||||||||||||||||||| ||||||||||||| || | |||
Sbjct 1574 -A--TTATGCGAAGTGCTGAAACATGGTGGAGTTCACACGACTTATTCATGTTCTGGTCA 1630
Query 1788 TGGAGATCATAACCTCAATGCTTCAGGTGTGAATGTTACTTCCTTGACTGAAGAGAAAAT 1847
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1631 TGGTGATCATAACCTCAATGCTTCAGGTGTGAATGTTACTTCCTTGACTGAAGAGAAAAT 1690
Query 1848 ACTAGATGATTGCAAG-CCTTGTATATCGAAAGAACTCCCTTGTAATTCTCGTGGAACTG 1906
|||||||||||||| ||| |||||||||||||| ||||| |||||||||||||||||
Sbjct 1691 TGTAGATGATTGCAAGACCT-GTATATCGAAAGAAATCCCTCATAATTCTCGTGGAACTG 1749
Query 1907 ATGAACTTTCCAGAAATGATGAAGAGAAGATTACTTTACCTGGTAAGGAGCTAGAGGAAC 1966
|||||| ||| | |||||||||||| ||||||| |||| |||||||||||||||||||||
Sbjct 1750 ATGAACGTTCTATAAATGATGAAGAAAAGATTATTTTATCTGGTAAGGAGCTAGAGGAAC 1809
Query 1967 AGTTATACAGTTATGGGTTTGAATCAGATCGTGGTTATGATCTATCTAGAGTAATAAAGG 2026
||||||||||||||||||||||||||||| ||||| ||||||||||||||||||| |||
Sbjct 1810 AGTTATACAGTTATGGGTTTGAATCAGATTGTGGTCATGATCTATCTAGAGTAATGAAGA 1869
Query 2027 AGCAAGTTG-GCAAAAGAAATTTGTGCGATGACGGGAAGGTCCAAGGACCAGCTGCCGTT 2085
||||||||| | |||| |||| ||| ||||||||||||||| ||||||||||||||||||
Sbjct 1870 AGCAAGTTGAG-AAAACAAATCTGTACGATGACGGGAAGGTTCAAGGACCAGCTGCCGTT 1928
Query 2086 TTCACGGAAAGTAATGAGGTTGCACAT-CCTGAGTGTGGTGGTTCTGAAACTGAACAAAG 2144
||||||||||| ||||||||||||||| || || | ||||||||||||||||||| |||
Sbjct 1929 TTCACGGAAAGAAATGAGGTTGCACATGCC-GAATACGGTGGTTCTGAAACTGAACGAAG 1987
Query 2145 GAACATTAATGTTCCATGCCATG-TCCACTTTCATAATTCTAACCATGTGGAAGAAAAAG 2203
|||||||||| |||| ||||||| | | ||||| ||||||||||| |||||||||||||
Sbjct 1988 GAACATTAATATTCCGTGCCATGGTG-ATTTTCAAAATTCTAACCACGTGGAAGAAAAAG 2046
Query 2204 GGAGTCAACCTGCACTTCTTGGTTATACAG 2233
||||||| ||||||||||||| | ||||||
Sbjct 2047 GGAGTCAGCCTGCACTTCTTGATAATACAG 2076
Score = 1240 bits (671), Expect = 0.0
Identities = 949/1075 (88%), Gaps = 52/1075 (5%)
Strand=Plus/Plus
Query 226 GACCCTCGAAGTTCAACTATGTCTGGAAGCCAAGAGCCTAGGATCAGACCATCTACATGG 285
|||||||| ||||| |||||||||||||||||||||||||||||| | |||||||| |||
Sbjct 4 GACCCTCG-AGTTCTACTATGTCTGGAAGCCAAGAGCCTAGGATCCGGCCATCTACCTGG 62
Query 286 AGCTGCAGTGATATTCCAATCAAGAAGAGGAAGTACCTTGTTCAGCCGCAAATGGAAGAA 345
||||||||||||||||||||||||||||||||||||||||| ||||||||| ||||||||
Sbjct 63 AGCTGCAGTGATATTCCAATCAAGAAGAGGAAGTACCTTGTGCAGCCGCAACTGGAAGAA 122
Query 346 GCTGTCTCCACTCAGATTCCACAACCTAATGAGCAAGGTGATACTA--GGAG-TGCT--C 400
|||| |||| | |||||||||||||||| ||||||||||||||||| || | || |
Sbjct 123 GCTGCCTCCTCACAGATTCCACAACCTAGTGAGCAAGGTGATACTAGTGGTGCTGGTAGG 182
Query 401 A-TG-C-T-GACGAAACTC-AGAAAATGACTGGTCGAGAACCAACCTCTTCATTACCATC 455
| || | | | | ||| || |||| || |||| || | | | |||||
Sbjct 183 AGTGCCTTGGTTTCATCTCTAGCCGATGA-AAATC-AGAA--AATGAC-T-GGT-CCATC 235
Query 456 TGTTCCTGTGGGAATTTCTGGTAAAGGGAAGAGCATTGGGAACA-TAGTTTTTGACCAAA 514
|||| ||||||||||| ||||||||||||||||||||||||||| || |||| |||||||
Sbjct 236 TGTTTCTGTGGGAATTGCTGGTAAAGGGAAGAGCATTGGGAACATTA-TTTTAGACCAAA 294
Query 515 CTAGAGTGAAATTTGAGAAGCCAAGTTCTCCAATTCACTCCAGTCCATTGGCAGGCTTTG 574
|||||||||| |||||||||||||||||||||||||||||||||| |||||||| ||||
Sbjct 295 TTAGAGTGAAAGTTGAGAAGCCAAGTTCTCCAATTCACTCCAGTCCTTTGGCAGGGTTTG 354
Query 575 ACATCCCTTCTAGTTCTAACGTACTTGGCAGTTCAATCCATT-TTCCTATGGGAAAGCTT 633
| ||||||||| ||| ||| ||||||||||||| |||||| || || |||||||||||
Sbjct 355 ATATCCCTTCTTCTTCCAACATACTTGGCAGTTCTCTCCATTGTT-CTCTGGGAAAGCTT 413
Query 634 CCTGTTGGTGCTGAACATGCTGGTCTTGTTGTCCCCTCAAAT---CAAACTCGGATGAAA 690
||||||||||||||||| ||||| |||||||| ||||| | |||||| ||||||||
Sbjct 414 CCTGTTGGTGCTGAACACCCTGGTGTTGTTGTCACCTCAGGTGCACAAACTGGGATGAAA 473
Query 691 GTAGAAAAAACTGTTCTTAAGACTCATGATA-TAGTC-CGGAAGACAG-GTGACAAGGAA 747
||||||||||| |||||||||||||||||| | ||| || |||| | |||||||||||
Sbjct 474 CTAGAAAAAACTCTTCTTAAGACTCATGATAGT-GTCAAGG-AGAC-GTGTGACAAGGAA 530
Query 748 ACTCTCAGAGGAGAGTGTCAAACAGAAGCATCTTCTGGTGCTAAGACTGTTTCCTTACAG 807
||||||||||||||||||| |||||||||||||||||||||||| |||||||||||||||
Sbjct 531 ACTCTCAGAGGAGAGTGTCTAACAGAAGCATCTTCTGGTGCTAATACTGTTTCCTTACAG 590
Query 808 CTAAGTTGTAACACTAAAAACAATTCTCCATATTGGAAGGATGAAGAGCCTACAGAACTG 867
||| |||||||||||||||||| |||||||||||||||| | ||||||||||||||||||
Sbjct 591 CTAGGTTGTAACACTAAAAACAGTTCTCCATATTGGAAGAACGAAGAGCCTACAGAACTG 650
Query 868 AATTTGTCATTAAGCAAGGGAGTTTGTCCCGCTCATAACACAGATTCTACTTCTAC---- 923
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 651 AATTTGTCATTAAGCAAGGGAGTTTGTCCCGCTCATAACACAGATTCTACTTCTACTTGT 710
Query 924 -C-AAATCTGGCAACAGTGGCCTGAACAGAGAAAATTGGGATTTGAATACTACCATGGAT 981
| ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 711 ACTAAATCTGGCAACAGTGGTGTGAACAGAGAAAATTGGGATTTGAATACTACCATGGAT 770
Query 982 GTTTGGGAAGATGCTCTAGATCGCAC-A--AG----A-G------G-TGCATTCTTAAAC 1026
| |||||||| ||||||||||||||| | || | | | |||||||||||||
Sbjct 771 GCTTGGGAAGGTGCTCTAGATCGCACGACTAGGGTTAAGACAACTGGTGCATTCTTAAAC 830
Query 1027 AGTAACAGAAGTCTTCGTGACATAGAGAGATCAAGTTGTCGTGATACGACTGCTATTACA 1086
||||||||||||||||||||||||||| ||||||||||| |||||||||||| |||| ||
Sbjct 831 AGTAACAGAAGTCTTCGTGACATAGAGGGATCAAGTTGTAGTGATACGACTGTTATTGCA 890
Query 1087 AAGTCTGTTTCTGAAAGACAGAAGGAAAGTGTAGGATTTAGTTCTCCTAAGGTGACGTTG 1146
|||||||||||||||| ||||||||||||||||||||||||||||||||||||||| |||
Sbjct 891 AAGTCTGTTTCTGAAAAACAGAAGGAAAGTGTAGGATTTAGTTCTCCTAAGGTGACTTTG 950
Query 1147 ATGCAGTTTGATAATCATGTTAATCCCACATGCTCACTTAGTCTAGGCCTCAGTTCATAT 1206
||||||||||||||||| ||||| |||||||||||||||| |||||||||||||||||||
Sbjct 951 ATGCAGTTTGATAATCAGGTTAACCCCACATGCTCACTTAATCTAGGCCTCAGTTCATAT 1010
Query 1207 CCTCCTATTGAGAAATCTCCTTCTCTACCAGCTACCACATCAGAGGCAAGAGCTG 1261
||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 1011 CCTCCTATTGAGAAATCTCCTTCTCTACCAGCTACCACATCAGAGACAAGAGCTG 1065
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 193104125402
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5