BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT5G60150.1
Length=3938
Score E
Sequences producing significant alignments: (Bits) Value
Ahg950771 jgi|Araly1|950771|scaffold_802658.1 3467 0.0
> Ahg950771 jgi|Araly1|950771|scaffold_802658.1
Length=3582
Score = 3467 bits (1877), Expect = 0.0
Identities = 2241/2419 (93%), Gaps = 16/2419 (1%)
Strand=Plus/Plus
Query 210 GCTCGAAAGAGAAGCTAGAAATGGATAAGGATCTCTTAGATATCTCTGGAGAAGACGACG 269
||||||||||||||| ||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 3 GCTCGAAAGAGAAGCCAGAAATGGACAAGGATCTCTTAGATATCTCTGGAGAAGACGACG 62
Query 270 AAGATAATTGGCTTCTGAAGAATACGCCGAAGAAAACTAATTCGAGCCGAGGAAAGAGTT 329
||||||||||||||||||| ||||||||||||||| | ||||| | |||| ||||||||
Sbjct 63 AAGATAATTGGCTTCTGAAAAATACGCCGAAGAAAGTTCATTCGGGACGAGAAAAGAGTT 122
Query 330 ATTTGAAATGCTCACCTCTCCAAATCCCTAGATCTAGTCGTATCGTTCCTACTCGGCCTC 389
||||||| ||||| |||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 123 ATTTGAATTGCTCGCCTCTCCAAATCCCTAGATCTAGTCGTATCGTTCCTACTCGGCCCC 182
Query 390 CTTTTTCCCCAATTGGAAGAGTAACAGGCACCAGCAACAACAGGGAGCAGCCATGCGCTT 449
||||||| ||||||||||||||||| || ||||||||||||| |||||||||||||||||
Sbjct 183 CTTTTTCACCAATTGGAAGAGTAACTGGTACCAGCAACAACATGGAGCAGCCATGCGCTT 242
Query 450 CAGTTGATACAGATAGTGTAGGAAAGGAGAATGCTAAAGTTGAATTGCCCAAATTGAGTG 509
| ||||||||||||||||| |||||||||||| |||||||||||||||||||||||||||
Sbjct 243 CTGTTGATACAGATAGTGTTGGAAAGGAGAATTCTAAAGTTGAATTGCCCAAATTGAGTG 302
Query 510 TGGAGCGACAACAGATGAAGAAGAAGAAAAAGAATGCAGGGTTTAATTTGCGCAAGAGTT 569
|||||||||||||||||||||||||||||||||||||||| |||||||||||||| ||||
Sbjct 303 TGGAGCGACAACAGATGAAGAAGAAGAAAAAGAATGCAGGATTTAATTTGCGCAAAAGTT 362
Query 570 TAGCATGGGATAGAGCTTTTTCAACGGAAGAAGGTGTTTTGGATTCATCTGAGTTATCTA 629
||||||||||| ||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 363 TAGCATGGGATCGAGCTTTTTCAACGGAAGAAGGTGTTTTGGATTCATCTGAGCTATCTA 422
Query 630 AAATAACTGGAACTGCTTGCC-ATCTTGGTGGAGATCGGTTAGCTGCCATTCAGGAAGAG 688
||||||||||||||||||||| || ||||||||||||||||| |||||||||||||||||
Sbjct 423 AAATAACTGGAACTGCTTGCCAAT-TTGGTGGAGATCGGTTACCTGCCATTCAGGAAGAG 481
Query 689 TACAGAGAGTCAATGTCAGCTTCCAAATGTA--A-TGTTTCACCAGGACTACAAGCACTT 745
| ||| ||||||||||||||||| ||||||| | |||||||||||||||||||||||||
Sbjct 482 TTCAGGGAGTCAATGTCAGCTTCTAAATGTACTAGTGTTTCACCAGGACTACAAGCACTT 541
Query 746 GAAGAAAACTTATTTAACGACTTGCCTGTGAATAGTAAAAACAGAGAGAAGAAGCTTGTC 805
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 542 GAAGAAAACTTATTTAACGACTTGCCTGTGAATAGTAAAAACAGAGAGAAGAAGCTTCTG 601
Query 806 AGTGGCATAATG-CCCAAGGAGCTGAGCATATCTAAAGTTCCTACTACCAAATCTGACCC 864
||| |||| | | || |||||||||||||||||||||||||||||||||| |||| ||
Sbjct 602 AGT-GCATCACGTAGCAGGGAGCTGAGCATATCTAAAGTTCCTACTACCAAACCTGAGCC 660
Query 865 AGTTACAGTTGGAAACAACATGAAAAGGACTACTCAAAGTCCAATCAAAGCGAAAAACAG 924
| ||||||||| ||||||||||||||||||||| |||||||||| |||||||||||| ||
Sbjct 661 ACTTACAGTTGCAAACAACATGAAAAGGACTACGCAAAGTCCAAGCAAAGCGAAAAAAAG 720
Query 925 TCAACCTACTCAATTAAAGAATTCTCAGAGGAGCTTAGGATCTGAAAGCTTCTCTAAAAA 984
||||||||||||| |||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 721 TCAACCTACTCAACTAAAGAATTCTCAGAGGAGCTTAGGATCTGAAGGCTTCTCTAAAAA 780
Query 985 CACTTCAAGCACCAAGAGTAAAACAAAATCCAGTCTGGCGAGTAAATCTTCAATTCCTAA 1044
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 781 CACTTCAAGCACCAAGAGTAAAACAAAATCCAGTCTGGCGAGTAAATCTTCAATTCCTAA 840
Query 1045 GCCCTCTCTTAAGCAAGCTAGAAGAAATGTGATCAGCAAATCTTCAGAAATTCCGACAGT 1104
|||||||||||||||||||||||||||||||||||||||| | ||||||||||| ||||
Sbjct 841 GCCCTCTCTTAAGCAAGCTAGAAGAAATGTGATCAGCAAAACATCAGAAATTCCTTCAGT 900
Query 1105 ATC-ATACTCACAGCATTCTGTTGTTGCAAAATCCAACGTTGGCCCTATGACTGCATCAG 1163
||| | ||||||||||||||||||||||||| ||||||||||||||||||||||| ||||
Sbjct 901 ATCCA-ACTCACAGCATTCTGTTGTTGCAAAGTCCAACGTTGGCCCTATGACTGCGTCAG 959
Query 1164 ATGTGGCTATGCTTGGTCATGCATCAGTTATTCCAGACTCTAATGTAATTACTCTGGGCA 1223
||||||||||| |||||| |||||| | ||||||||| || ||||||||||||||||| |
Sbjct 960 ATGTGGCTATGTTTGGTCCTGCATCGGATATTCCAGAGTCCAATGTAATTACTCTGGGAA 1019
Query 1224 CCTCGTTAGCTCAAAGTTCTTGCAATAAAGCT-GGAAGCACGCAGTCCGCAGTTTCACGA 1282
|||||||| |||||||||||||||||| | || |||| ||||||||||||||||||||||
Sbjct 1020 CCTCGTTAACTCAAAGTTCTTGCAATAGA-CTTGGAAACACGCAGTCCGCAGTTTCACGA 1078
Query 1283 CTGGGAAAGCCATCAGGCTTAAGAGCGCCAAAACCTTCAATTGGCTATTTCAGTCAGTCT 1342
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 1079 CTGGGAAAGCCATCAGGCTTAAGAGTGCCAAAACCTTCAATTGGCTATTTCAGTCAGTCT 1138
Query 1343 GACTCTCAACCGTCGCAGAGTGCGGGAGACAAACACAGTCAGCTTCCCAGATCTGATGTT 1402
|||||||||| |||||| ||||| ||||||||||| ||||||||||||||||||||||||
Sbjct 1139 GACTCTCAACTGTCGCACAGTGCAGGAGACAAACATAGTCAGCTTCCCAGATCTGATGTT 1198
Query 1403 TGTTCAGCCGCTCATTTTTCTTTGATCCCTACCTTCAAGAAACCCCAAGTTGCTGAAAAG 1462
| ||||||| ||||| |||||||||||||||||||||||||||| |||||||||||||||
Sbjct 1199 TCTTCAGCCTCTCATATTTCTTTGATCCCTACCTTCAAGAAACCTCAAGTTGCTGAAAAG 1258
Query 1463 TTTCCCGGTGTCAACTGCAAGGCAGCAACTGGAATTTTTGGCAGTTCAGATTCAGCTGCA 1522
|||| |||||||| | |||||||||||||||||||||||||||||||| ||||||||||
Sbjct 1259 CTTCCTGGTGTCAAATCCAAGGCAGCAACTGGAATTTTTGGCAGTTCAGGTTCAGCTGCA 1318
Query 1523 AGATTCTCTGCTCAATCTATATGTCTCAAACCTTCACAGGAAAAGTTGAAAGTGGACTTG 1582
|||||||||||||||||||||||||||||| ||||||| |||||| ||||||||||||||
Sbjct 1319 AGATTCTCTGCTCAATCTATATGTCTCAAATCTTCACAAGAAAAGGTGAAAGTGGACTTG 1378
Query 1583 AATAGCACACAGGAAGTAGAATCCAAAGTGTTGCGTTGTCCATTGAGTTTTCAGATAAAT 1642
|||||||| ||| || |||||||||| | |||| ||||| || |||||| ||||||||||
Sbjct 1379 AATAGCACTCAGAAATTAGAATCCAATGCGTTGTGTTGTTCACTGAGTTCTCAGATAAAT 1438
Query 1643 GAAAATCCGCAGCACCAA-TGTGTCATTCAAGGTGATACTGGAAATTTAGTTCTGGATGA 1701
||||||| |||||||||| | || ||||||||||||||||||||| || ||||||||||
Sbjct 1439 GAAAATCTGCAGCACCAAAT-TGCCATTCAAGGTGATACTGGAAAAGTACTTCTGGATGA 1497
Query 1702 TGTAACTTATTGCACATCTGAGAAGATTTCTACTGAACAATGTCAAGAATTCCAGGGTAA 1761
|||||||||||| ||||||||||||| |||||||| ||| |||||||||||||| ||||
Sbjct 1498 TGTAACTTATTGTACATCTGAGAAGAATTCTACTG--CAA-GTCAAGAATTCCAGAGTAA 1554
Query 1762 CAGTGCGCTGCCGCCCTCAGGCTGCAAAAATAATGTACAAGATGGTAGTAATATGTCTGA 1821
|| || ||| |||| ||||||||||||||||||||| || ||||||||||||||||||||
Sbjct 1555 CAATGAGCTACCGCTCTCAGGCTGCAAAAATAATGTGCAGGATGGTAGTAATATGTCTGA 1614
Query 1822 CGATAATAGAGATGAGAAAAGGAAGTCTTGCCTCTCAGTAGAAGAATACTGTGCTTTACC 1881
| |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct 1615 CCATAATAGAGATGAGAAAAGGAAGTCTTGCCGCTCAGTAGAAGAATACTGTGCTTTACC 1674
Query 1882 AATGAAAGATTCTATGGATTCTACGATGCAGGGTCCTCCTTGCGATGAGTTGACACTGTT 1941
||||||||||| |||||||| | |||||||||||||||| ||||||||||||||||||
Sbjct 1675 GCTGAAAGATTCTGTGGATTCTCCAATGCAGGGTCCTCCTTCCGATGAGTTGACACTGTT 1734
Query 1942 TGACAACTACTCACAGTTGAAAGTCAGCAATCCGGGGGAAGAAGATATGTGTACTACCAA 2001
||| | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1735 TGATACCTACTCACAGTTGAAAGTCAGCAATCCGGGGGAAGAAGATATGTGTACTACCAA 1794
Query 2002 TGATTTTAGTGGAGATTCTGATACATTGGATGTACCTGGACAACCTCTAAATGAATGCCT 2061
||||||||||||||||| ||||||||||||||||| |||||||||||||||||||||| |
Sbjct 1795 TGATTTTAGTGGAGATTTTGATACATTGGATGTACATGGACAACCTCTAAATGAATGCGT 1854
Query 2062 GCACCCTGGAAACGAAGACGAGATTAGTCCTTGCCTTTCTGAAGAAAAAGATGCGTTAGT 2121
|||||||||||| |||||||||||||||||||||||||||| ||| | ||||| ||| |
Sbjct 1855 GCACCCTGGAAATGAAGACGAGATTAGTCCTTGCCTTTCTGGTGAAGATGATGCTTTAAT 1914
Query 2122 TGTTTATCATTCTACTGAATATGTAGCAAAGCAGCCTGAGGTTTTGGATTCCTTCACTGC 2181
||| |||||||||||| || ||||||||||||||||||||||||| ||||| |||| ||
Sbjct 1915 TGTGTATCATTCTACTAAAAATGTAGCAAAGCAGCCTGAGGTTTTATATTCCGTCACCGC 1974
Query 2182 AAAATCATCTTTCTTTGGGGATTTGGCTTCAATAAAGGGTGATGCAGACAATCCATCTTC 2241
| | || ||||||||| ||||| ||||||||||||||||||| |||||||| ||||||
Sbjct 1975 AGACCCAACTTTCTTTGAAGATTTTGCTTCAATAAAGGGTGATGTAGACAATCTATCTTC 2034
Query 2242 TTCAGAAAATCAGTTAGGTAATACTGAATTTGTTTCTGTACCTTTGGAGCCTTCTGAAGC 2301
||||||||||| ||||||||||||| ||| |||||| ||||||||| ||||||||||||
Sbjct 2035 TTCAGAAAATCGGTTAGGTAATACTAAATATGTTTCCATACCTTTGGGGCCTTCTGAAGC 2094
Query 2302 ACTAGATTGTGTTCAATCACTATGCAATCATCTTGAGGTAAATGCGGTAGCGAACTCTGT 2361
|| ||| ||||||||||||| ||||||||||||||||| ||||||||| ||||||||| |
Sbjct 2095 ACCAGAATGTGTTCAATCACCATGCAATCATCTTGAGGAAAATGCGGTGGCGAACTCTAT 2154
Query 2362 TTTGTGTGATCATAACATGGTGTGTGATGGTCAATCTGTATTAGAAACGGAGAAGAGAAT 2421
|||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2155 TTTGTGTGATCATAACATGGTGTGTGATGGTCAAGCTGTATTAGAAACGGAGAAGAGAAT 2214
Query 2422 TGAAATAAGCGAAAGCACAGAGAAAAACTATGAGACTGATTTCATTGGTCCATTTTCCGA 2481
|||||||||||||||||||||| ||||| |||| ||||||||||||||||| ||||||
Sbjct 2215 AGAAATAAGCGAAAGCACAGAGACAAACTGTGAGGCTGATTTCATTGGTCCAAGTTCCGA 2274
Query 2482 ATGCAAATACTGGTTTAGAGAATCTGAAGAGCAACATTTGTCGAACCAATTAGTCCTTGA 2541
||||||| |||||||||||||||||||||| ||||||||||||| |||||||||||||||
Sbjct 2275 ATGCAAAGACTGGTTTAGAGAATCTGAAGAACAACATTTGTCGAGCCAATTAGTCCTTGA 2334
Query 2542 GGTGAAAGAGGGTGGTCATGAGATAGATGTCTTGATCAGAAACGAAGAAGCTGATGGGCC 2601
|||||||||||||||||||||| | |||||||||||||||||||||| ||||||||||||
Sbjct 2335 GGTGAAAGAGGGTGGTCATGAGTTCGATGTCTTGATCAGAAACGAAGGAGCTGATGGGCC 2394
Query 2602 TGATATGCAGATAGAATGC 2620
|| |||||||||||||||
Sbjct 2395 TGGCATGCAGATAGAATGC 2413
Score = 1716 bits (929), Expect = 0.0
Identities = 1094/1174 (93%), Gaps = 10/1174 (1%)
Strand=Plus/Plus
Query 2671 GCCTTCC-TCCGTTGAAGTTACGATGGAGATTAAACCTCTGGAATCATCTCATGAACCCT 2729
||||||| |||||||||||| |||||||||||| |||||||||||||||||||||||||
Sbjct 2412 GCCTTCCACCCGTTGAAGTTAGGATGGAGATTAACCCTCTGGAATCATCTCATGAACCCT 2471
Query 2730 TCTCTGAGAAATCAACTTCTGAAAAACAGAAGCAATACAATTGTTCCAGCAGTGAAAATA 2789
||||||||||||||||| ||||||| ||||||||| ||||||||||||||||||||||||
Sbjct 2472 TCTCTGAGAAATCAACTCCTGAAAAGCAGAAGCAACACAATTGTTCCAGCAGTGAAAATA 2531
Query 2790 CATCTGATGTAAATGATGGTTGTGTAATGAAACAGGCTGATCAACTTGGAACATTAGTTG 2849
|||||||||||||||||||||||||||||||||| || ||||||||||||||||||||||
Sbjct 2532 CATCTGATGTAAATGATGGTTGTGTAATGAAACAAGCGGATCAACTTGGAACATTAGTTG 2591
Query 2850 GTTGTTCTCCAGAAAAAGATGCATCTGT-TGCGGTTTTCTCTTATTCAAATGAGGAGCTT 2908
||||||||||||||||||| |||||| | ||| |||||||||||||||||||||||||||
Sbjct 2592 GTTGTTCTCCAGAAAAAGACGCATCT-TCTGCTGTTTTCTCTTATTCAAATGAGGAGCTT 2650
Query 2909 GGAGATAACTCTGAGCTGGAAGACATGGATTTGGTTACAGACTCAGATTGTTCAGATGAG 2968
| |||| ||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2651 GAAGATTACTCTGAGCTGGAAGACATGGATTTGGGTACAGACTCAGATTGTTCAGATGAG 2710
Query 2969 GAGCCTGAGGATAAATTGGAGCGGACAGAGGTGGATGTGGTTACTGATCCAGAGCTCATT 3028
||| |||||||||| ||||||||||||||| || || |||||||||||| | |||||||
Sbjct 2711 GAGGCTGAGGATAATTTGGAGCGGACAGAGATGCTTGGGGTTACTGATCCTGTGCTCATT 2770
Query 3029 AGTGGCCTTGATGAGTTTTCTGTCAAGGGCGTCCGTAATCAAGAATATCCGGAAGTAGAA 3088
|||| ||||||||||||||||||||||||| | | ||||||||||||||| ||| ||||
Sbjct 2771 AGTGACCTTGATGAGTTTTCTGTCAAGGGCATTCTTAATCAAGAATATCCCCAAGAAGAA 2830
Query 3089 GATATCCAAACTGCATCAGACCTTTGTGGAAAAACTAAAACTCTACTAAGTGAATCAGTC 3148
|||||||| |||||||||||||| |||||||||||||||| ||||||| |||||||| ||
Sbjct 2831 GATATCCATACTGCATCAGACCTGTGTGGAAAAACTAAAAATCTACTACGTGAATCAATC 2890
Query 3149 AGTTCCTCGGATTCTATATTTGGAGTTCCTGAGTGTCTAAATGAGGGCACAAACTTTTCC 3208
||||||||||||||||||||||||| |||||||||||||||||| |||||||| ||||||
Sbjct 2891 AGTTCCTCGGATTCTATATTTGGAGATCCTGAGTGTCTAAATGAAGGCACAAATTTTTCC 2950
Query 3209 AGAATCTCTGAAGGCATGTCAAAAGAGGATTCAAATTCTGGACGTATTGAGCATAACTAT 3268
||||||||||||| |||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 2951 AGAATCTCTGAAGACATGTCAAAAGAGGATTCAAATTCTGGACGTATTGAGCATAAATAT 3010
Query 3269 GTTGTAAAAGCTGAATTCCAGGTAGATGCTGAAAAGGATTTCAGTGCACAGGTTACTGGT 3328
|||||||||||||||| |||| |||| |||||||| |||||||||||||||||||||||
Sbjct 3011 GTTGTAAAAGCTGAATACCAGATAGAAGCTGAAAAAAATTTCAGTGCACAGGTTACTGGT 3070
Query 3329 CAAGAACTCGTCCCCAATGAAGGAGATGAAGTTAAAGTTGTAAAGATCTCTCCTGATCCT 3388
||||||||||||||| |||| ||| | |||||||||||||||||||||||||||||||||
Sbjct 3071 CAAGAACTCGTCCCCGATGAGGGAAAGGAAGTTAAAGTTGTAAAGATCTCTCCTGATCCT 3130
Query 3389 GTA-TCATTTGCGCCCAGAGAGGAAGAACT-AGGAACTCCAATACCCATGAAGGAGACTA 3446
||| | |||||||| ||||||||||||| | ||||||||| ||||||| || ||||||
Sbjct 3131 GTAGT-ATTTGCGCTCAGAGAGGAAGAA-TCAGGAACTCCTATACCCACAAAAGAGACTT 3188
Query 3447 TCTCAGGCAGAGATGATATGCAGATTAACGAGTTCAATGTACTCAGCGATGACATTTTGA 3506
|||||| |||||||||||||||||||| |||||||||||||||| ||||||||| |||||
Sbjct 3189 TCTCAGTCAGAGATGATATGCAGATTAGCGAGTTCAATGTACTCTGCGATGACAGTTTGA 3248
Query 3507 CATCAGAAAGCAATGCATCAGAAGGCAATGACAAGATGATACTACTGGATGCTAAACTTG 3566
||||||||| |||||||||||||||||| || |||||||||||||||||| | |||||||
Sbjct 3249 CATCAGAAACCAATGCATCAGAAGGCAAGGAGAAGATGATACTACTGGATACGAAACTTG 3308
Query 3567 AGAAGAAACCTGATCCAATAATTGTGAAGCCTCCAAATGCTGTTCCATTTTCTGACGAGT 3626
|||||||||||||||| |||||||| ||||||||||||||||||||||||||||| ||||
Sbjct 3309 AGAAGAAACCTGATCCTATAATTGTAAAGCCTCCAAATGCTGTTCCATTTTCTGATGAGT 3368
Query 3627 GGTTAGCTGCAATTGAGGCTGCTGGGGAGGAAATTTTAACACTGAAAAGTGGACGTGTGC 3686
||||||||||||||||||||||||||| |||||| |||||||||||||||||||||||||
Sbjct 3369 GGTTAGCTGCAATTGAGGCTGCTGGGGGGGAAATCTTAACACTGAAAAGTGGACGTGTGC 3428
Query 3687 AACATTCACCAACCGAGAAATCTGCTCCTGAACCAGGTCCATGGTCTCCGGTC---AAGA 3743
|||||||||||||||||||||||||||||||||||||||| |||||||||||| ||||
Sbjct 3429 AACATTCACCAACCGAGAAATCTGCTCCTGAACCAGGTCCTTGGTCTCCGGTCAAGAAGA 3488
Query 3744 AGAGCAACCAAGTTGTAGGACCATTTGACTGTACAAAGTATAATAACAAAGGTCTTCCTC 3803
||||||| ||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3489 AGAGCAATCAAGTTGTAGGGCCATTTGACTGTACAAAGTATAATAACAAAGGTCTTCCTC 3548
Query 3804 CTGCTTTTGACTGAAGAAAACTAGGTTTCTTCTA 3837
||||||||||||||||||||||||||||||||||
Sbjct 3549 CTGCTTTTGACTGAAGAAAACTAGGTTTCTTCTA 3582
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 148843168003
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5